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The comparison of IR spectrum and X-ray diffraction and waxy crystal size for paraffin, gum ,and asphaltene before and after treatment was made.

用降凝降粘剂EVA+AA处理高蜡原油,并对原油处理前后的石蜡、胶质、沥青质进行红外特征吸收峰、X射线衍射峰以及蜡晶尺寸变化研究。

The papA gene was amplified from the pilus adhesive cluster of UPEC132, and then was cloned into plasmid pUC18. The recombinant plasmid was identified and subjected to DNA sequencing.

采用此引物扩增132菌株p菌毛粘附基因群的papA基因,扩增产物克隆入质粒,选取阳性重组质粒进行核苷酸序列分析。

The effect of coconut amine on the viscosity and stability of residue asphaltene system was studied and the change in viscosity and colloid stability with coconut amine content was discussed briefly.

考察了表面活性剂椰子胺对石油沥青质体系粘度和胶体稳定性的影响,初步探讨了粘度和胶体稳定性随椰子胺质量分数变化的原因。

Adhered intension of passivation film: scrub film 2 to 3 second by soft rubber, then check scratch mark on the film.

2.2钝化膜的粘附强度:检验有色钝化膜的粘附强度时,可用一块软质橡皮在镀层表面摩擦2至3秒钟,镀层膜上应无刮伤痕迹。

The rheological parameters of sullage soft-soil are tested, and the influencing characteristics and changing disciplinarian of clay and water percentage to the initial shear stress and viscosity are analyzed.

测试了淤泥质软土的流变参数,分析了粘粒含量、含水率等因素对初始剪切力、粘度等流变参数的影响特性和变化规律。

In this study, cross-linked type I and type II collagen matrices, with and without attached chondroitin sulfate, were implanted into full-thickness defects in the femoral trochlea of adolescent rabbits.

本研究中,粘附和不粘附硫酸软骨素的交联 I型和II型胶原基质植入到青年兔股骨髁的全层缺损。

There are three primary types of wear:(1) abrasie, in which the harder of the surfaces produces grooes in the softer material,(2) adhesie, in which the softer material is transferred as a thin film onto the harder surface, and (3) fatigue, in which repetitie loading produces subsurface cracks and particles, or sheets of material subsequently delaminate and are lost from the surface.

磨损主要有三种类型:1、研磨,硬质表面在软质材料上造成沟槽;2、粘着,软质材料形成薄膜移覆于硬质物体的表面;3、疲劳,反复负载造成材料表面断裂及颗粒形成,或材料出现层裂并从表面脱落。

Several points can be concluded for the area from this paper study:(1) Hydrocarbon mainly occurs in the lower interval of bioclastic limestone and dolomitic limestone(the special lithologic interval ) in Es_1 Member,in which solution pores well developed;(2) Traps are mainly anticlines controlled by f.

本文研究表明,大中旺地区油气主要分布在沙一段下部的生物碎屑灰岩和云质灰岩段,储层溶蚀孔隙发育;圈闭主要为受断层控制的断背斜构造;原油具有高密度、高粘度、中低凝固点、高初馏点、高含硫量和高胶质沥青质等特点,属于未成熟—低成熟油;油源为本地咸水环境下形成的沙一段暗色泥岩。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank,选择包含人主要T细胞抗原表位序列的人PSCA基因片段,应用异种化抗原设计技术,保留人T细胞抗原表位,设计异种化PSCA融合抗原片段;(2)根据核酸序列按中心模板法设计引物,应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因,以PCR、限制性酶切和DNA序列测定法进行鉴定:(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点,采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4),并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中,转染293T细胞,借助免疫荧光+流式细胞术考察插入片段表达效率,最终选定PSCA_3片段进行下一步研究;(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下,插入pVAX1-neo-IRES—GM/B7载体中,构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB,并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况;(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞,制备小鼠前列腺癌模型,并采用股四头肌肌肉注射+电脉冲法(Electroporation,EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB,同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照,通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率,来评价该DNA疫苗在小鼠体内的抑瘤效果。

Hematocrit and blood viscosity at shear rate in 150/s, 30/s, 5/s, 1/s, renal fibrosis serum markers, serum creatinine and blood urea nitrogen were detected respectively in sham operation and model group; microcirculation microscope was used to observe the thread of hypoglottis and auricle in rats.

建立单侧输尿管梗阻致大鼠肾间质纤维化模型,观察肾间质纤维化大鼠全血粘度150/s切变率、30/s切变率、5/s切变率、1/s切变率、红细胞压积、舌下及耳廓的脉络变化,同时检测肾纤维化指标、血清肌酐及尿素氮。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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