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Xanthus DK1622 by PCR, and inserted the fragment into a site upstream of lacZ, resulting in the recombinant plasmid pZCY13. The plasmid pZCY13 was transformed by electroporation to DK1622, producing a mutant ZC16-18 ?

从黄色粘球菌DK1622基因组上PCR扩增MXAN1334基因内部部分片段,插入载体pZCY11上lacZ基因的上游,构建重组质粒pZCY13,将其转入DK622菌株,获得MXAN1334基因插入失活突变株ZC16-18。

Tamil Nadu Agricultural University, Coimbatore: The objective is to maximize production of rice-green gram in black clay loam typical Haplustalf at Coimbatore. The treatments consisted of combinations of plant population (5.0×105 and 8.0×105 hills/ha), fertilizer levels (150-50-50, 200-50-67, 250-50-83 kg N-P2O5-K2O/ha) and splits in fertilizer application (3, 4 and 5 splits) to rice. The residual effect of these treatments is being tested on green gram. Another MYR experiment on rice-rice-green manure has been planned at Aliyarnagar involving factors of plant population, fertilizer levels and their splits.

泰米尔纳德农业大学:目的在于在哥印拜陀的黑粘壤质土(典型的弱发有半干润淋溶土)条件下使稻-绿豆耕作制达到最高产量,处理包括水稻的密度(5.0×105和8.0×105蔸/公顷)、施肥水平(150-50-50、200-50-67、250-50-83公斤 N - P2O5- K2O/公顷)和施肥次数(3、4和5次)的组合,这些处理的后效通过绿豆进行试验,另一最高产量研究试验区计划在 Aliyamagar 进行,试验作物为稻-稻-绿肥,包括有种植密度、施肥水平和次数三个试验因素。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。