粘细胞

The mechanism of PPS accelerating IEC-6 cells migration was also researched with gene-chip technique. The Altas Clontech Rat cDNA Chip which consisted of 1176 spots representing different functional categories such as heat shock/stress, cell cycle, transcription factors, hormones, metabolism, etc was used. Result showed in cell migration model of IEC-6 cell line, compared with control group, expression of 38 genes in total 1176 genes varied in cells treated by PPS, including 21 genes up-regulated and 17 genes down-regulated. Date analyze showed the mechanism of PPS accelerating IEC-6 cells migration mainly related with promoting the numbers and activity of cellular ion channel, especially 〓 channel.

从以上实验结果中我们可以得出以下结论：四君子汤总多糖具有促进IEC-6细胞增殖、迁移、粘附的作用；四君子汤总多糖无论是对IEC-6细胞增殖、或者迁移的作用均优于各单味药多糖，可能是各种单味药多糖综合作用的结果；多糖可能是四君子汤"益气健脾"药效的主要成分；小肠上皮细胞可能是四君子汤总多糖的作用靶点之一，提示临床采用四君子汤及"益气健脾"法治疗"脾虚证"的疗效机制可能与增强机体的整体免疫和肠道粘膜免疫功能有关。

The cells were preincubated at first in liquid phase for 24 hours at a concentration of 5×106 nucleated cells/ml of RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and concanavalin A5μg/ml of culture medium.

先将脾脏有核细胞(5x10~6/ml)在含有ConA(5μg/ml)的液相中培养24小时，然后收集非粘附细胞，将其接种到含0.88％甲基纤维素、10％FCS，ConA 5μg/ml和15％ConA-LCM的半固体系统中培养，细胞的终浓度为5x10~5/ml。

The integrinα_6β_1-LN interaction is probably a local regulator of the ovary. LN may play an important role in granulosa cell luteinization during the periovulatory phase through interfering the function of LN/hCG receptors and enhancing the progesterone production of GCs.

LN通过与细胞表面粘附分子整合素α_6β_1(integrinα_6β_1)相互作用，可能为卵巢内的一种局部调节因素，干扰LH/hCG受体功能并增强颗粒细胞孕酮的产生，在围排卵期颗粒细胞黄素化过程中发挥重要效应。

Vasodilator-stimulated phosphoprotein is one of these proteins, it locale at most motility place on the cell membrane.

目前最新资料提示，一种特异性细胞骨架相关蛋白，Vasodilator-stimulated phosphoprotein，能够调节细胞粘附和伸展过程中细胞骨架actin纤维的重排。

Methods: cationic liposome lipofectamine2000 was used to transfect retrovirus vector pbabe pten puro containing pten and pbabe puro into pten deficient prostate cancer cell line pc 3 respectively, and then cell growth, adhesion, segregation, apoptosis, cell cycle and ultrastructure alteration in vitro were studied.

利用阳离子脂质体lipofectamine 2000介导，分别将含pten的逆转录病毒载体pbabe pten puro及空载体pbabe puro转入pc 3细胞，观察转染前后细胞的生长、粘附、超微结构、细胞周期改变情况。

METHODS: The rib trabeculae were resected and broken, trypsinizated and washed completely by PBS. Bone surface and non-adhesive floating cells in cleaning fluid were observed with inverted microscope. Rib trabeculae was washed by DMEM culture medium once, and cultured in culture bottle. The culture liquid was replaced by new one once a week. The osteoblast was moved from the sclerite a week later. The cells were fused monolayer and could be subcultured 4 to 6 weeks later.

切下人肋骨骨小梁剪碎，胰酶消化后以PBS彻底冲洗直至于倒置显微镜下观察骨片表面及清洗液中无粘着及漂浮的细胞，以DMEM完全培养液清洗骨小梁1次，移入培养瓶内培养，每周更换培养液1次，1周后可见成骨细胞自骨片移出，4~6周细胞融合成单层可作传代培养。

The results show:(1) In testis, PNA receptors sites were firstly observed in the cytoplasm and plasma membrane of primary spermatocyte, and greatly increased in the plasma membrane of second spermatocyte, then decreased and were accumulated in the tail of the round spermatid. The same reaction was observed in sperms of testis and spermatheca, which shows stronger reaction in head and weaker in tail. A universal dyeing was observed on the plasma membrane of sertoli cells.

结果表明：(1)在中华蚱蜢的精子发生中，PNA受体是由初级精母细胞合成，然后转移到膜上，在次级精母细胞，由于量的积累其质膜出现了强阳性标记；从早期精子细胞到成熟精子时期糖蛋白的分布发生了明显的修饰变化，这些变化与精子获得与卵子进行识别、粘附、结合等受精能力密切相关：受精囊内与精巢内的精子表面的标记是相同的，说明质膜糖蛋白的修饰作用在精巢内就已完成。

Result: Schwann-like cells disperse in the hole of nerve graft at oneweek under Scanning Electronic Microscope, the processes of thesatellite-like cells adhere to the wall of graft.There was no statisticaldifference of activity of cells in both group(P＞0.05) using MTT. At 12weeks,the result of appearance, histology and TEM were similar, therewere no statistical difference in SFI%, NEP and quantitative analysis ofrecovery rate of myelinated fiber populations, diameter of myelinatedfiber and thickness of myelin sheat.

结果：1w后扫描电镜下可见接种的类Schwann细胞分散在材料表面的孔隙内，细胞形态为星状，细胞突起与支架管壁粘附；MTT法测定细胞活性示神经支架组在各时间点与对照组无显著性差异P＞0.05；12w时两组动物坐骨神经外观、组织学光镜观察、透射电镜观察结果相似、两组坐骨神经功能指数恢复率、神经电生理、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率等指标相比无显著性差异(P＞0.05)。

The significantly increase of ICAM-1 mRNA expression on alveolar epithelium and pulmonary venular endotheium was also found in asthmatic rats compared with those of normal and sensitized control, but the expression of ICAM-1 mRNA on pulmonary arteriolar endothelium and bronchial epithelium showed a inceasing tendency.

原位杂交结果表明，哮喘组大鼠肺静脉内皮细胞、肺泡上皮细胞ICAM-1 mRNA表达量明显多于对照组；支气管上皮细胞、肺动脉内皮细胞粘附分子表达也有增加的趋势。

Methods Based on the model of human umbilical vein endothelial cell,this study adopted Rose Bengal Stain,cell ELISA,immunocytochemistry techniques to investigate the effect of APS on lymphocyte-endothelium adhesion and the molecular mechanism.

以体外培养的人脐静脉内皮细胞为模型，应用蛋白染料染色、细胞ELISA、免疫细胞化学等方法，研究黄芪多糖对淋巴细胞与血管内皮细胞粘附的影响及其分子机制。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失