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One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活,ICAM-1表达升高,与被激活的PMN表面表达的LFA-1相结合,"PMN-EC"相互作用加剧,一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍;另一方面粘附的PMN过度吞噬或激活,当白细胞吞噬的颗粒不能被封闭隔离,连同细胞内的酶被释放出来,其中的PMN-elastase能够降解细胞基质中各种成分,水解多种蛋白,加重胰腺的毛细血管内皮细胞和腺泡的损伤;释放的溶酶体酶使组织蛋白水解,产生的不饱和脂肪酸引发脂质过氧化方应,破坏细胞膜的结构和功能;释放的炎性细胞因子,激发其他的免疫细胞的功能,导致进一步的组织损伤和坏死,加重SAP的病理损伤,最终导致休克、脓毒血症及多器官功能障碍等严重后果。

This account for cytoskeleton and cellular morphology and adhering capacity could be affected by alterative culture medium in the process of the culture.

说明细胞培养过程中,更换细胞生长环境可影响到细胞骨架,进而改变细胞形态及细胞的粘附能力。

The method uses radiative immunity law to determine CV group, healthy group and content of the SIgA in taking secretion of bacterium group vagina, method of the conglutinate outside using system determines white beads bacterium is epithelial to the vagina (the conglutinate ability of VEC), the influence of VEC of conglutinate of white beads bacterium when observing SIgA standard.

方法采用放射免疫法测定CV组、健康组及带菌组阴道分泌物中sIgA含量,用体外粘附方法测定白念珠菌对阴道上皮细胞的粘附能力,观察sIgA水平时白念珠菌粘附VEC的影响。

Proliferation assay, cellular cycle and apoptosis analysis, invasion and migration assay and adherency assay showed that GEBP11 could inhibit the proliferation of Co-HUVECs and HUVECs, induce the apoptosis of ECs, but not alter the cell cycle of ECs.

MTT细胞增殖实验、细胞周期及凋亡分析、细胞侵袭迁移实验、细胞粘附实验显示,GEBP11短肽对Co-HUVECs及HUVECs的增殖表现出不同程度的抑制作用,对Co-HUVECs抑制作用更强,GEBP11能诱导内皮细胞的凋亡,而对细胞周期无明显影响。

METHODS: The different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育,倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数;将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育,分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

Keratin antibody IgM 3B4 specifically against Candida albicans in vitro. METHODS: The different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal anti??keratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti??

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育,倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数;将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育,分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

The soil and bark samples collected from Yunnan, Hebei and Shanxi provinces of China, and also from Vietnam, were used as the materials in this experiment. And dung pellets of rabbits or goats that had been autoclaved can be used to induce many kinds of fruiting bodies of the Myxobacteria in different colors, sizes, and shapes. We isolated and purified Myxobacteria strains with the improved method which was found by our laboratory.

本实验以采集自越南以及我国的云南、河北、陕西各地的土样和各种树皮、腐木作为实验材料,利用兔、羊粪球和滤纸作为基质诱导出多种颜色丰富、形态多样的粘细菌子实体,采用本实验室摸索出的较为有效的纯化粘细菌的方法,在实验器材上用24孔细胞培养板代替传统的平皿,有效地缩短了粘细菌的纯化周期,提高了分离纯化的效率。

The characteristic in liquid culture of the purified strains were described, and the relation between the characteristic and the taxonomic traits was researched for the first time. The two groups of Myxobacteria differentiated according to the shape character of the myxospores and vegetative cells had different characteristic in liquid culture.

对纯化菌株的液体培养特征进行了描述,并首次依据初步分类鉴定结果探索了液体特征与分类地位之间的关系,即依据营养细胞和粘孢子形态将粘细菌划分成的两大类型中,第一类型(包括粘球菌属等7个属)的菌株在液体培养基中通常形成片状或絮状,而第二类型(包括单囊菌属等5个属)的菌株则主要呈现球形。

The myxobacteria have a life cycle that is dominated by social behavior. During vegetative growth, cells prey on other bacteria in large groups by gliding motility. When faced with starvation, cells form a macroscopic fruiting body containing thousands of resting myxospores.

粘细菌运动、发育相关基因的克隆及分析粘细菌以具有复杂的多细胞社会学行为著称,它们能够在营养丰富的条件下在固体表面上滑行运动;又能在饥饿的条件下聚集形成子实体结构以及抗逆性的粘孢子。

From 94 soil samples and 9 bark samples in these areas, 259 Myxobacteria strains were isolated and 140 strains of them were purified. These 259 Myxobacteria strains were primarily identified in genus or species level by their fruiting bodies, myxospores and vegetative cells on the base of Sergey's manual of determinative bacteriology and they belonged to 9 genera(Myxococcus, Corallococcm. Angiococcus. Archangium, Cystobacter. Stigmatella . Hapoangium. Nannocystis. Polyangium) of Myxococcales.

根据菌株的特征性子实体形态和其营养细胞、粘孢子形态,以《Bergey's manualof determinative bacteriology》第九版为依据,将分离纯化出的菌株初步鉴定到属,这259株粘细菌菌株经过初步的分类鉴定,分属于9个属,即粘球菌属139株、珊瑚球菌属20株、囊球菌属18株、原囊菌属21株、孢囊杆菌属5株、标桩菌属36株、单囊菌属9株、小囊菌属3株,和多囊菌属8株。

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