粘细胞

The fluidity or viscosity of cell surfaces is an extremely complex subject, and the degree of viscosity has to be appropriate for the function of the cell.

流动性或粘度的细胞表面是一个极其复杂的课题，和程度的粘度要适当的功能细胞。

ObjectivesThe aim of this study was to assess the influence of holothurian glycosaminoglycan on the adherence of thrombin-induced platelets to human umbilical vein endothelial cells in a flow chamber system.

海参糖胺聚糖抑制血小板—内皮细胞粘附及其机制的研究目的血小板-内皮细胞粘附在血栓形成，尤其在动脉血栓形成中占有举足轻重的地位。

3 Effects of the components of YYTJ on the adhesion of leukocyte to HUVEC The effect of components of YYTJ (APS, Perlolyrine, Cinnamylaldehyde and Muscone) on the adhesion of PMN or THP-1 to HUVEC by TNF-primed, as well as the expression of ICAM-1, VCAM-1 and CD44 on HUVEC was measured respectively.

2.3 疡愈涂剂各组分对白细胞与血管内皮细胞粘附影响的分子机制观察疡愈涂剂各组分黄芪多糖、川芎嗪、桂皮醛和麝香酮对HUVEC和PMN活力的影响，PMN、THP-1与TNF诱导的HUVEC粘附作用及HUVEC表面ICAM-1、VCAM-1和CD44表达的影响。

Methods: THP-1 cells were induced by oxLDL in vitro. The CD11b, CD54 expressions and adhesion to human umbilic al vein endothelial cells were measured after treatment with probucol at different concentrations by flow cytometry and β-nitrophenyl N-acetyl-β-D -glucosminide test.

分别应用流式细胞仪和β-N-乙酰氨基己糖苷酶比色法，观察不同浓度普罗布考对oxLDL诱导的THP-1对人脐静脉内皮细胞的粘附及粘附分子CD 11b和CD54表达水平的影响。

The combination of viscometry with flow cytometry and mathematical analysis as presented here represents a novel approach to differentiating between the effects of hydrodynamics and the intrinsic biological processes that control cell adhesion .

与流式细胞仪和数学分析粘度这里所提出的组合代表了两者之间的流体力学效应和生物过程的内在控制细胞粘附与众不同的新方法。

Objective To study the mechanism of monocyte recruitment in atherogenesis and to clarify the effect of monocyte chemotactic protein-1 (MCP-1) in this process.

目的 了解动脉粥样硬化发病过程中单核细胞粘附并进入动脉内膜的机制，分析单核细胞趋化蛋白-1（MCP-1）基因表达对单核细胞粘附动脉内膜的作用。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Recently ICAM-1 has also been shown to be the cellular receptor for the major group of rhinoviruses and subtertian malaria infected red cell.

目的：检测细胞间粘附分子-1(ICAM-1)在正常肝组织和肝脏肿瘤组织及细胞中的表达情况，进行人细胞间粘附分子-1的cDNA克隆及建立稳定的转染细胞株。

METHODS:Based on the model of human umbilical vein endothelial cell, this study adopted Rose Bengal Stain, cell ELISA, immunocyto-chemical techniques to investigate the effect of berberine on PMN-endothelium adhesion and the expression of cell adhesion molecules.

以小檗碱加IL-1、TNF处理人PMN或人脐静脉内皮细胞后，用蛋白染料染色法研究小檗碱对PMN与HUVEC粘附的作用，用细胞ELISA、APAAP法研究小檗碱对细胞表面粘附分子表达的影响。

METHODS: The presence and number of recently developed extra-articular manifestations and a weighted EAM score, as well as the levels of serological markers, were compared between 31 patients with RA with histologically proven vasculitis and 50 patients with RA in whom vasculitis could not be documented histologically. The following markers were evaluated: circulating immune complexes, complement components C3 and C4, class-specific rheumatoid factors, antineutrophil cytoplasmic antibodies, antinuclear antibodies, antiendothelial antibodies, circulating intercellular adhesion molecule-1 and -3, circulating vascular cell adhesion molecule and E-selectin, cellular fibronectin, von Willebrand factor antigen, and C reactive protein.

对比组织学上已证实有血管炎的31例 RA 例和组织学上无血管炎的50例 RA 患者间的临床及血清学标记物，这些资料包括最近发生的关节外表现数量、关节外表现加权得分、循环免疫复合物、补体 C3和 C4、类风湿因子（ IgM-RF，IgG-RF，IgA-RF ）、抗中性粒细胞胞浆抗体、抗内皮细胞抗体、循环细胞间粘附分子-1和-3、循环血管内皮粘附分子和 E-选择素、细胞纤连蛋白、假性血友病因子抗原（ von Willebrand factor antigen ）及 C-反应蛋白。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。