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粘细胞

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While the β-catenin homologus Aardvark of Dictyostelium is absent, adherent junctions are lost. Aar also has a function in cell signaling and regulates expression of pre-spore gene.

盘基网柄菌β-连环蛋白同源物Aardvark的缺乏使细胞间失去粘著连接,Apr也有信号转导功能,调控了前抱子细胞基因的表达。

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因,用PCR、测序鉴定缺失菌株,通过动物实验观察clpE基因缺失株毒力改变情况,同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力,最后用实时荧光定量PCR分析自溶素(major autolysin A,lytA)、表面黏附素A(pneumococcal surface adhesion A,psaA)、溶血素(pneumolysin,ply)、肺炎球菌表面蛋白A(pneumococcal surface protein A, pspA和神经氨酸酶(neuraminidase, nanA)的表达。

Cells can stick to the basement membrane of acellular dermal matrix well.

脱细胞真皮基底膜具有良好的细胞粘附性。

In addition, the expression of VEGF and MMP-2 were analyzed by western blot.

MTT比色法检测细胞对细胞外基质的粘附能力;5。

Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型,检测气道压力峰值—时间指数、支气管/肺泡灌洗液中炎性细胞数量,PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平,免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

Objective:To explore the effect of Ravan Napas on the expression of ICAM 1 and HO 1 on broncho epithelial cells of asthmatic rats.

目的:研究维药纳气平喘颗粒对哮喘大鼠支气管上皮细胞细胞间粘附分子(ICAM 1)和血红素氧合酶 1(HO 1)表达的影响,以阐明纳气平喘颗粒治疗哮喘病的部分作用机制。

Result: Rhesus BMSC can be got in mass by density gradient centrifugation.; Rhesus BMSC can adhere to PLGA fiber quickly after plant and its growth , division ,proliferration is good; Some cells arrange like rosary on PLGA fiber surface, some cells still form the catenate or flaky cell bridge joined among PLGA fiber.

结果:采用密度梯度离心的方法可得到大量增殖能力强的猕猴骨髓基质干细胞;接种后的骨髓基质干细胞很快粘附于PLGA纤维上,生长、分裂、增殖良好,在PLGA纤维表面形成串珠样排列,部分细胞还形成连接在PLGA纤维间的链状或片状细胞桥。

The 2 activation that share cell of antigen particularity T and enlarge add signal, by the generation after the conglutinate of APC and the CD28 element interaction that stimulate factor CD80, CD86 and T cell in all.

信号2参和抗原特异性T细胞的活化和扩增,由APC的粘附和共刺激因子CD80、CD86和T细胞的CD28分子相互功能后产生。

The results suggest that exterior of nomal PAM has plenty of microruga, the surface area of PAM was increased so as to make it possible to conglutinate and englut more conidia when activated by Aspergillus fumigatus conidia; at the same time, it provides the histologic evidence of augmentation of lysosome and endoplasmic reticulum.

我们发现,正常PAM 表面有大量微皱褶或微皱襞,在被烟曲霉孢子激活后,PAM 可以明显增大细胞的表面积,即可以使一个巨噬细胞同时粘附和吞噬多个

Following results have been obtained: a novel method of surface modification of transparent silicon rubber with plasma to adapt it to cell adherence and spread was developed, and a set of elastic matrix cell culture devices based on the method was developed; it is found that MSCs oriented 90 degrees to the applied cyclic strain field and aligned, the reaction of MSCs to the cyclic stretch is related to the loading regimen and aging of MSCs, cyclic strain may lead to differentiation of MSCs.

主要研究结果有:研究出利用等离子体对硅胶进行表面改性以利于细胞粘附和铺展的新方法,并基于此研制出了可动态加载的应变均匀的新型弹性基底细胞培养装置。发现MSC在受到交变拉伸加载后沿与受力垂直的方向排列的现象,该现象与加载的条件和MSC的老化程度有关,交变拉伸加载可能有促进MSC分化的趋势。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。