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The adhesive capacity of differentiated cells that stably expressed E-cadherin was markedly enhanced. Compact cell connection and multi-layer growth state remained at 19 days. While non-transfected ESCs gradually changed from embryoid bodies into noncohesive cell populations.

稳定表达上皮型钙粘蛋白的分化细胞间黏附能力明显增强,并在19 d时仍能保持致密的细胞连接和多层生长状态,普通胚胎干细胞则由拟胚体结构逐渐分化为松散的细胞群落。

We found that the reduced cell density and pseudopodia compared with control indicated the inhibition of cell growth and poor movement after IFN α-2b treatment.

采用光镜、电镜观察该细胞在含不同浓度干扰素α-2b 培养液中的生长形态和粘附移动能力。结果:干扰素α-2b 能够使该细胞生长密度降低、伪足收缩、细胞变圆、移动能力减弱。

This experimental study, based on the ischemia-reperfusion injury model of the isolated rabbit liver, was to investigate hepatocellular apoptosis and its mechanism during cold preservation-reperfusion of donor liver and assess the structural and functional damages of the reperfusing donor liver subsequent to rewarm ischemia. In addition, the expression and effects of ICAM-1 mRNA were mainly observed during rewarm ischemia-reperfusion of donor livers. Our experiment included three parts: The first was establishment of the isolated liver ischemia-reperfusion injury model in rabbits.

本实验在建立离体供肝缺血-再灌注损伤模型的基础上,研究供肝低温保存-再灌注过程中肝细胞凋亡现象及其相关机制;探讨作为临床肝移植中供肝的另一缺血阶段—复温缺血对再灌注后供肝结构及功能的损伤作用,并着重观察供肝复温缺血-再灌注期间组织细胞内细胞间粘附分子-1 mRNA的表达规律及其作用。

These critical spermatozoal functions are acquired in the epididymis where a specific luminal environment is created by the blood-epididymal barrier; proteins secreted by epididymal principal cells bind to maturing spermatozoa and regulate the maturational process of the spermatozoa.

精子功能在附睾特殊的管腔环境中获得,这一环境是由血-附睾屏障来维护。附睾主细胞分泌的蛋白与精子结合并调控精子的成熟。附睾中细胞-细胞间的相互作用通过粘附连接和紧密连接形成。

They can reach 72.04% and 84.5% separately(P.05). 2. The methods such as Percoll uncontinuous density gradient centrifugation, isolation according to the different adhesiveness can be used to isolate and purify rabbit SSC effectively. Spermatogonia mainly distribute between the gradient 27% to 35%(density between 1.0410g/ml to 1.0508g/ml).

Percoll 不连续密度梯度离心和差异粘附等方法可以有效的对兔精原细胞进行分离纯化,其中精原细胞主要分布于密度梯度为27%-35%(密度 1.0410g/ml~1.0508g/ml)的带间,此带中精原细胞的纯度平均达到 60.42%(P.01)。

The results showed that though testosterone or 17-β-estradiol did not affect the expression of these AM in unstimulated HUVEC, both of them transiently increased the expression of E-selectin and VCAM-1 in TNF-α-stimulated HUVEC.

近几年研究发现,粥样硬化与炎症及免疫反应有关,TNF-α等细胞因子可以通过刺激血管内皮细胞粘附分子的表达及血管平滑肌细胞的增殖等而在粥样硬化的形成中起重要作用。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中,雏鹅临床表现为高发病率和高死亡率,食欲和饮水减少,拉白色、黄绿色稀粪,伴有神经症状;常规组织学观察,脾脏和胰腺肿大,有白色坏死斑点,肠道出血和坏死,肝脏肿大变性,肾脏肿大变性有尿酸盐沉着等;用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究,结果表明:胰腺中发现病毒包涵体,肠道中大量纤维素以及胶原纤维增生,肾脏、肺脏大量含铁血黄素沉着,免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少;电镜下多种细胞呈现线粒体水肿,多种实质细胞的染色质固缩,染色质边集,特别是细胞内容物如脂肪滴增多的病理变化特征。

Methods After Candida albicans were inoculated into the specific pathogen free mice, the total amount of Candidas in cecum and the amount of which attached to the mucosal membrane were counted at different interent intervals. The lymphocyte proliferation in Peyer's patch and in lamina proper was shown by BrdU incorporation, meanwhile B cells isotype switch in PP was investigated. IgA plasma cells were shown by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.

采用无特殊病原菌动物经口喂入白念菌,在不同时相处死后,观察肠内白念菌总数及肠粘膜表面白念菌粘附数量;取肠系膜淋巴结做白念菌选择培养,观察移位体内发生率;采用Brdu体内掺入显示局部粘膜淋巴细胞的增殖情况;流式细胞计数潘伊尔氏结细胞表面IgA阳性率;免疫组化染色后计数固有层中IgA浆细胞数量变化;ELISA法测定肠粘液中特异抗白念菌IgA含量。

MUC3 apomucin and its mRNA were mainly situated in the cytoplasms of goblet cells and columnar-type cells of duodenum and column.But no reactive signal of MUC2 and MUC3 genes' product was found in the mucous droplets.

MUC3核粘蛋白及其mRNA主要存在十二指肠和结肠的杯状细胞和柱状细胞胞浆内,而杯状细胞的粘液滴内无MUC2、MUC3基因产物的阳性反应信号。

The viscosity range between the inside of the cell to the outside of the cell is about 36 dynes per cm2 depending upon the cell hydration and other factors.

细胞内和细胞外的粘滞度范围约为每平方厘米36达因,这取决于细胞水化状态和其它因素。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。