粘细胞

4DRG was co-culture with sciatic nerve segment in 10鸖 DMEM;the axons were longer and surround the sciatic nerve segment which was regard as anew evidence for chemotropism.

结果：(1)在无血清条件下单独培养的DRG，背根神经节的轴突数目众多，外形纤细弯曲，不成束，并且施万细胞和成纤维细胞稀少，所以可以排除两者对轴突生长的影响，为观察来源于变性坐骨神经段的可溶性因子对轴突生长的作用提供了有利条件；(2)在无血清条件下DRG和变性坐骨神经段联合培养，①先单独培养DRG，4天后待神经元轴突长出，再与坐骨神经段联合培养，观察到神经元的轴突数目减少，外形挺直，部分轴突之间相互粘附成束；②变性坐骨神经段和DRG同时联合培养，神经元的轴突数目明显减少，外形粗壮，轴突之间相互粘附成束；(3)有血清条件下单独培养DRG，轴突数目较多，外形挺直，长短不一，部分神经元的轴突之间相互粘附，施万细胞和成纤维细胞数目众多，观察到的轴突生长情况受到施万细胞和成纤维细胞的直接或者间接的影响。

Methods Proliferation,cytotoxicity in vitro and expression of CD11 c of the cells cultured in the two different media were compared.To observe the tumor target cells killed by adˉherent natural killer cellsthrough electroscope.

分别用AIMV及完全培养基培养粘附NK细胞和非粘附NK细胞，比较细胞的增殖能力、杀伤肿瘤细胞的能力、表达粘附分子CD11 c 的能力，并通过电镜对A-NK细胞所杀伤的肿瘤细胞进行形态学观察。

Objective To compare serum-free medium AIMV with standard serum-containing medium in culturing IL-2-activated natural killer cells in vitro.Methods Proliferation,cytotoxicity in vitro and expression of CD11 c of the cells cultured in the two different media were compared.To observe the tumor target cells killed by adˉherent natural killer cellsthrough electroscope.

目的 研究方面比较无血清培养基AIMV与完全培养基对体外细胞因子激活的NK细胞的支持作用方法分别用AIMV及完全培养基培养粘附NK细胞和非粘附NK细胞，比较细胞的增殖能力、杀伤肿瘤细胞的能力、表达粘附分子CD11 c 的能力，并通过电镜对A-NK细胞所杀伤的肿瘤细胞进行形态学观察。

To begin with, the adhesive experimental model between SMMC-7721 cells and HUVECs was established in this paper, then expression of integrin β〓 in SMMC-7721 cells was determined by flow cytometer, finally, adhesive mechanical properties of different cell cycle SMMC-7721 cells to collagen Ⅳ were studied by micropipette aspiration technique.

本文首先建立了肝癌细胞与内皮细胞粘附力分析的实验模型，接着应用流式细胞技术检测肝癌细胞上integrin β〓的表达，最后研究了不同细胞周期肝癌细胞与内皮细胞的粘附力学特性。

Now, I mainly engaged in myxobacterial genomic and bioinformatics analysis work. Myxobacteria is a single-cell microorganisms, but they act as multi-cell behavior (such as feeding, social movement, cells congregating and fruiting body formation, and so on) and own a wealth of secondary metabolites Product, those remarkable characteristics make myxobacteria important in the research fields of prokaryotes cell differentiation and development, biological evolution, as well as the drug development and research.

现主要从事粘球菌基因组与生物信息学分析方面的研究工作：粘细菌是单细胞微生物，然而却具有多细胞行为（如摄食、社会性运动、细胞聚集和子实体形成等）和丰富的次级代谢产物，这些行为特征使粘细菌在原核生物的细胞分化发育、生物进化以及药物开发研究中占有重要地位。

Results PE was found to be able to stimulate the proliferation of murine splenocyte in the absence of accessory cells. Accessory cell and its supernatant from AC stimulated with PE was able to enhance the proliferation of lymphocyte induced by PE.

结果 PE在0.01～100ng/ml浓度范围能刺激鼠脾淋巴细胞增殖，PE的丝裂原作用无需粘附细胞的辅助，但粘附细胞及其粘附细胞的PE刺激上清能协同PE对鼠脾淋巴细胞的刺激作用。

The capacity of endothelial cells to recognize and migrate in response to the extracellular matrix depends on one or more members of the integrin family of cell adhesion receptors.

血管新生的过程依赖于参与血管生成的细胞因子和细胞粘附的协同作用，整合素是一组细胞表面受体，介导细胞-细胞间、细胞-基质间的相互作用，对内皮细胞的粘附、迁移、增殖起重要作用。

Results:Berberine inhibited HUVEC adhesion for lymphocyte when pretreated HUVEC and antagonised IL-1 and TNF-induced upregulation of HUVEC adhesion for lymphocyte.Meanwhile,IL-1-stimulated lymphocyte adhesion to HUVEC was diminished in the presence of berberine.The main molecular mechanism of berberines function may be downregulating the expression of ICAM-1 on the HUVEC .

结果：小檗碱能抑制静息的及IL-1、TNF激活的内皮细胞与淋巴细胞间的粘附，其主要分子机制为下调内皮细胞表面粘附分子ICAM-1的表达；小檗碱能抑制IL-1激活的淋巴细胞与内皮细胞的粘附，对淋巴细胞表面粘附分子CD18的表达无影响。

Results:Berberine inhibited HUVEC adhesion for lymphocyte when pretreated HUVEC and antagonised IL-1 and TNF-induced upregulation of HUVEC adhesion for lymphocyte.Meanwhile,IL-1-stimulated lymphocyte adhesion to HUVEC was diminished in the presence of berberine.The main molecular mechanism of berberine's function may be downregulating the expression of ICAM-1 on the HUVEC .

结果：小檗碱能抑制静息的及IL-1、TNF激活的内皮细胞与淋巴细胞间的粘附，其主要分子机制为下调内皮细胞表面粘附分子ICAM-1的表达；小檗碱能抑制IL-1激活的淋巴细胞与内皮细胞的粘附，对淋巴细胞表面粘附分子CD18的表达无影响。

Cellular adhesion molecule is a kind of glucoprotein expressed on the membrane surface of various cells,which mediate the conjunction and adhesion of cells and extracellular matrix.Vascular cellular adhesion molecule-1(VCAM-1) and intercellular adhesion molecule-1 (1CAM-1) are members of CAM.

细胞粘附分子(cellular adhesion molecule，CAM)是一类由多种细胞产生的，调节细胞和细胞间以及细胞和基质间相互结合并起粘附作用的膜表面糖蛋白，主要包括血管细胞粘附分子-1(vascular cellular adhesion molecule-1，VCAM-1)和细胞间粘附分子-1(intercellular adhesion molecule-1，ICAM-1)等。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失