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Electron micrograph showed us that hepatocytes were damaged seriously along with the prolonged NHBD time in A group. At the NHBD 120 and 150min in A group, hepatocytes showed numerous cytoplasmic vacuoles and lysosomes, round swollen mitochondria with clear matrix and rough endoplasmic reticula which was not in a stacked arrangement.

电镜发现,A组随着心跳停搏时间的延长,肝细胞的损伤逐渐加重,至120和150min时,肝细胞内可出现大量空泡和溶酶体,线粒体和粗面内质网结构也遭到破坏,但未出现明显的坏死和凋亡改变。

Results: The cultured chondrocytes were polygonal cells. There were many rough endoplasmic reticula and mitochondria in cytoplasm, and a lot of secretory vesicles under cell membrane and in the cytoplasm. When cultured for 10 days, some small and white nodules were formed on the bottom of the culture dished, and volcanic-mouth-like structures were formed when cultured for 20 days. Both these nodules and structures contained GAG-positive substances were demonstrated by safranine-O staining.

结果:体外培养的软骨细胞为多角形,透射电镜下见胞浆内有丰富的粗面内质网系统及线粒体,胞膜下及胞浆中有较多分泌泡;连续培养10天时,培养瓶底部出现肉眼可见的白色结节,蕃红-O染色显示白色结节含大量氨基葡聚糖阳性物质,20天时形成火山口样结构,也被蕃红-O染成深红色。

The results showed that,in spiteof many ultrastructural changes of the fruit epidermis cells during the fruit development,itremained almost unchanged that the cytoplasm was filled with numerous endoplasmic reticulathroughout the whole developmental process.Most of these endoplasmic reticula were tube-likeand rough-ER with enlarged cisterna from which were produced many vesicles. Some of thevesicles were shown to merge into vacuole and to traverse through plasmadesmata.

结果表明,虽然不同发育期果皮细胞的超微结构发生了变化,然而最引人注目的是,内质网自始至终密布于整个细胞质中,而且大多为槽库膨大的、合成功能旺盛的管状粗面内质网,并分泌出大量的具运输功能的小泡:观察到这些小泡与液泡融合和穿越胞间连丝的景象。

The primary culture chondrocytes were round or polygonal shape, half of the 4th generation turned to spindle shape, most of the 6th passage were long spindle shape; The rough endoplasmic reticula and the mitochondrial were rich and well developed, the ability of proliferation of the 3th generation were stronger than the first one, while the 5th generation was weaker.

原代软骨细胞呈圆形或多角形,第4代有一半变成梭形,第5代绝大部分变为长梭形;原代软骨细胞胞浆内粗面内质网和线粒体丰富,第3代软骨细胞生长增殖能力强于原代,第5代增殖能力明显减弱。

Fig 4 Few of rough endoplasmic reticula, mitochondrid and co llagen can be seen in the cytoplasm of fibroblast, the ribosome of rER is degran ulated, the nucleous is abnormal, there are a lot of granule (maybe the antisens e oligodeoxynucleotide) in the cytoplasm TEM×5200

图4反义核酸组的瘢痕成纤维细胞粗面内质网较少、有脱颗粒现象,线粒体少,胞核畸形,胞内有吞噬颗粒,细胞周围胶原纤维较少TEM×5200

A lot of organelles such as mitochondria, rough endoplasmic reticula and Golgi bodies were observed in fiber cell. Then, double karyotheca disappeared, and the organelles disintegrated. Multivesicular bodies appeared in fiber cytoplasm. With the further development of fiber, fiber wall underwent continual thickening with aging, and polylaminate structure gradually appeared. While the agglutinated nucleus, transfer vesicles, plasma membrane and plasmodesmata still presented.

研究发现,次生壁形成早期,细胞核具有双层核膜,染色质凝聚,可见大量的线粒体、粗面内质网和高尔基体等细胞器存在于纤维细胞中;随后,双层核膜消失,细胞器将逐渐解体,多泡体开始出现在纤维细胞的细胞质;随着年龄的增加,纤维细胞壁逐渐增厚,并出现多层结构现象,而运输小泡、细胞膜、胞间连丝和凝聚的染色质将持续存在。

In electron microscopy endothelial cell of capillary and mitochondrial was edematous obviously, the basement membranes were thickened. RGCs were vacuolated, chromatin of nucleus aggregated marginally decreased and densification. Some of RGCs were necrosis, nucleus were dissolved, mitochondria was edematous, the rough endoplasmic reticula decreased obviously.

电镜下视网膜毛细血管内皮细胞明显肿胀,胞体变圆,突向管腔,线粒体肿胀,空泡化,基底膜增厚,视网膜神经节细胞胞体变形,核明显固缩,核膜消失,胞浆空泡化,线粒体肿胀,细胞表面突起减少,多聚核糖体及粗面内质网显著减少。

The techyte, formed during the time of ES3 (38.0~43.0 Ma) and with better stored property for oil and gas, has the characters of high alkalies (K2O+Na2O=10.22%~12.47%), high potassium (K2O/Na2O=0.69~1.88), high aluminium (Al2O3=17.82%~19.44%) and low titanium (TiO2=0.19%~0.67%), and belongs to the typical shoshonite series.

粗面岩的K-Ar年代学和地球化学表明,它们形成于沙三时期(38.0~43.0Ma),具有富碱(K2O+Na2O=10.22%~12.47%)、富钾(K2O/Na2O=0.69~1.88)、高铝(Al2O3=17.82%~19.44%)、低钛(TiO2=0.19%~0.67%)的特点,属于典型的钾玄岩系。

At the age of 19 weeks, we have observed ciliated-mucous cells, some cells presented with mucous granules in the supranuclear cytoplasm and a ciliating border with short growing shafts, while the other cells presented with large mucous granules as well as compound cilia.

小粘液颗粒细胞呈柱状,胞浆顶部含有少量球形的粘液颗粒,细胞表面有短小的微绒毛,细胞核大呈椭圆形,胞浆电子密度高,胞浆下部有粗面内质网和线粒体,核上部有高尔基体。

Rock types include basalt,trachybasalt, alkaline basalt, andesite and andesibasalt, which belong to alkalinity-weak alkalinity rock series, and give priority to neutrality-basic volcanicrock, that indicate the tectonic setting of continental rift.

岩石类型为玄武岩、粗面玄武岩、碱性玄武岩、安山岩和玄武安山岩,属于碱性-弱碱性岩系,以中性-基性火山岩为主,指示出大陆裂谷型构造背景。

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