粗制的

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

Funalia gallica, Lenzites tricolor, Polyporellus brumalis, Pseudotrametes gibbosa, Pycnoporus sanguineus, tested their degrading ability to david poplar wood lignin, measured the lignin content change of david poplar wood after a period of time degraded by 6 wood rot fungi, which is the first study on the biodegradation mechanism of lignin by wood white rot fungi.

本项研究选择了火木层孔菌及另外5种木材分解能力较强的阔叶树上的白腐菌：粗毛盖菌、三色革裥菌、冬拟多孔菌、偏肿拟栓菌和血红密孔菌，研究了它们对山杨木材木质素的分解能力，测定了经6种白腐菌分解一定时期的山杨木材木质素的含量，作为木材白腐菌对山杨木材木质素生物降解机制的初步研究，旨在为山杨木材生物制浆造纸提供应用基础理论研究，同时也可为木质素合理的生物转化为有用的化学品、生物漂白、酶处理防止机械浆的返黄、废水治理、纤维素酶解糖化的微生物前处理等提供相关的借鉴研究，以期在生产实践中减轻环境污染并充分利用木质素资源。

Jin Shui HighSPEED Mill Power Producing System can make the differdnt molal structures mate rials powderfor 20 eyes to 80eyes in common temperaeure, These materials are differdnt kinds of soft hard PVC 、PP、PS; thosecan change its nature such as PP、TEP、 PS、 PA、 PC ,etc; non-plastic such as dee rhm, timber, leather and palm oil shells, etc,Especially suit for raw material of plastic to chang its naure, paints of mother colour tofill up , paints, tec .During the working process, firstly put the quantitative and well-distrib uted rawmaterialsun to the Moll Box by the Hopper, them put the powder out of the Moll box by Auto loader, finallysirfand retrieve the powder through sifting machine .If someofthe have not milled well , you can do itagain As a matter of fact, it will be as excellent as the mew plastic and can keep every normof the munchang eablein their function of chemistry and physicsafterusing about 30% of power mixed up with 70%raw mate rials of plastic. So the machines if helpful of plastic businessman to save money and good for protecting our environ mentathe same time

金穗JS 系列高精密磨粉生产系统能在常温下将各种的软硬：PVC、 PP 、PS 及改性PP 、PS 、PA 、PC 等不同分子结构的及非塑料类如（鹿角、木梢、皮革及棕油壳等）材料磨制成20目~80目粉末状，适合塑料原料改性，色母料填充涂料、塑纺织的涂料、地毯背面涂料、制药等加工行业，这些颗粒原料通过定量的加料器均匀地送进磨箱中，再由吸风机将磨好的粉末吸出磨箱经过筛选机筛选回收，如较粗的颗粒再次被送进磨箱中进行粉磨，这些材料经过我们特殊改良刀齿，创新突破来配合日新月异原材料组合使用，经塑料制品厂商实践证明在其产品原料中加入磨制粉体30%左右使其更好塑化且其制品的化学，物理性能保持全新料的各项指针不变，因此是塑料厂商降低成本且同时有利于保护环境的粉磨回收设备。

In Shui HighSPEED Mill Power Producing System can make the differdnt molal structures mate rials powderfor 20 eyes to 80eyes in common temperaeure, These materials are differdnt kinds of soft hard PVC 、PP、PS; thosecan change its nature such as PP、TEP、 PS、 PA、 PC ,etc; non-plastic such as dee rhm, timber, leather and palm oil shells, etc,Especially suit for raw material of plastic to chang its naure, paints of mother colour tofill up , paints, tec .During the working process, firstly put the quantitative and well-distrib uted rawmaterialsun to the Moll Box by the Hopper, them put the powder out of the Moll box by Auto loader, finallysirfand retrieve the powder through sifting machine .If someofthe have not milled well , you can do itagain As a matter of fact, it will be as excellent as the mew plastic and can keep every normof the munchang eablein their function of chemistry and physicsafterusing about 30% of power mixed up with 70%raw mate rials of plastic. So the machines if helpful of plastic businessman to save money and good for protecting our environ mentathe same time

S 系列高精密磨粉生产系统能在常温下将各种的软硬：PVC、 PP 、PS 及改性PP 、PS 、PA 、PC 等不同分子结构的及非塑料类如（鹿角、木梢、皮革及棕油壳等）材料磨制成20目~80目粉末状，适合塑料原料改性，色母料填充涂料、塑纺织的涂料、地毯背面涂料、制药等加工行业，这些颗粒原料通过定量的加料器均匀地送进磨箱中，再由吸风机将磨好的粉末吸出磨箱经过筛选机筛选回收，如较粗的颗粒再次被送进磨箱中进行粉磨，这些材料经过我们特殊改良刀齿，创新突破来配合日新月异原材料组合使用，经塑料制品厂商实践证明在其产品原料中加入磨制粉体30%左右使其更好塑化且其制品的化学，物理性能保持全新料的各项指针不变，因此是塑料厂商降低成本且同时有利于保护环境的粉磨回收设备。

The beginning of their work the more obstinate insistence Han Guan different regions within the shear system of the traditional methods and aesthetic interest, which affects not only the local style of the original, paper-cut patterns on the Mainland also a symbol of the cultural influences which generate half-breed exotic culture - Yin and Yang both,刚柔相济, crude see fine art in the show's Heilongjiang paper cutting alone.

她们的作品开始较为固执地坚持着关内汉族不同区域的传统剪制方法和审美趣味，这不仅影响到本地原有的风格，也把内地剪纸纹样的文化象征渗透入其中，生成异域文化的混血儿——阴阳并举、刚柔相济、粗中见细艺术独秀的黑龙江剪纸。

The products were identified by IR,NMR,MS and elemental analysis. In our method,K.OH was used in place of NaOH to synthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate was purified on a silica gel column with chloroform/ethylacetate(4:l,v/v) as eluent and further purified by triturating with hexane/acetone(2:l,v/v).The luminescence produced by DMAE-NHS is a flash light with maximumemission at 0.4s and decay half-time of 0.9s. The luminescence intensity is 6.11x10 cps/mol,which is affected by the composition of trigger and surfactant.

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿/乙酸乙酯(4：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，第二次柱层析用己烷/丙酮(2：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷/丙酮(2：1，Ⅴ：Ⅴ)进行研磨，过滤，洗涤，除去溶于己烷/丙酮(2：1，Ⅴ：Ⅴ)的部分，得到所需要的产品。

Western and the steak cooking methods, material selection is very particular about the materials used: First, the Varieties of the requirements of cattle in the absence of pollution of the ecological environment in the stock of natural-born beef cattle, cattle at the age of 2 to 4 years old, processing methods Hours are rough, fine, fine multi-channel operations; each has a different process secret spices and Chinese medicinal system, the processing of steak flavor from the inside out, Ruwei fishes, great flavor.

与西餐的牛排烹制方法相比，选材用料十分讲究：首先是牛的品种要求在没有污染的生态环境中自然放养的土生肉用牛，牛的年龄在2~4岁之间，加工方法也分粗、细、精多道工序；每道工序都有不同的秘制中药配方及调料，所以加工出的牛排味道由内而外，丝丝入味，鲜味无比。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。