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Flow cytometric also used for analysis the levels of reactive oxygen species, Ca2+ and mitochondrial membrane potential in both cell lines. The results showed Gyp promoted ROS and Ca2+ production and decreased the MMP level. Western blotting show that Gyp treatment gradually decreased the levels of anti-apoptotic protein (Bcl-2), but increased the levels of the pro-apoptotic protein.

除此之外,也藉由流式细胞仪分析测得经绞股蓝皂甘作用后的人类血癌细胞(HL-60)和小鼠血癌细胞(WEHI-3)里的活性氧化物浓度上升、钙离子释放以及粒线体膜电位下降等细胞凋亡重要指标,此结果可经由西方墨点法指出绞股蓝皂甘能使癌细胞中的抗凋亡蛋白Bcl-2表现量下降;促凋亡蛋白Bax表现量上升,同时也藉由共轭焦显微镜观察到致死蛋白AIF、Endo-G从粒线体释放至细胞核。

Gyp promoted the release of cytochrome c from mitochondia based on the changes of MMP and the activation of caspase-3 before leading to apoptosis. We also used confocal laser microscope to show AIF and Endo-G are released from mitochondrial then move to nuclei

根据上述多种实验结果可证实:绞股蓝皂甘能引起细胞中粒线体膜电位改变导致凋亡蛋白Cytochrome c从粒线体释放出而活化了caspase-3,进而诱发人类血癌细胞株(HL-60)和小鼠血癌细胞株(WEHI-3)走向凋亡路径

Pollen morphology of the Chinese monotypic genus Dichotomanthes and its out-group Prinsepia utilis were investigated from the point of view of pollen development. The SEM observation revealed that there is not any change in morphology and sculpture of pollen grains from the young pollen grain seperated from callose of tetraspore to the mature pollen grain in Dichotomanthes tristaniaecarpa. Only the volume of pollen grain increased with maturity.

从发育的角度研究了中国特有单种属Dichotomanthes Kurz及与其系统学研究有关的外类群Prinsepia utilis的花粉形态,扫描电镜观察显示Dichotomanthes花粉粒自脱离四分体胼胝质膜开始至成熟二核花粉粒不同发育时期,花粉形态和外壁纹饰未见变化,仅花粉体积随成熟度增加而有所增大。

Brown rhizomorph and hyphae can be seen on the seedlings'rhizome, main root and side root while nothing is on the tip of the root.It was found that rhizomorph on the surface of roots would die after inoculation in 90 days in pot.

子实体单生或丛生,出菇至成熟3 d~4 d,单个子实体重20.0 g~62.0 g;小粒咖啡苗的根茎、主根及侧根被茶褐色的菌索和菌膜包裹,而根尖及靠近根尖的侧根上没有菌丝和菌索生长或生长很少;盆栽小粒咖啡苗,在接种90 d后其根系表面的菌索死亡。

The results showed that with the increase of salt concentration,the chloroplast of oats expanded,the slices of basal granule were distorted or even disintegrated,matrix was tenuity,the starch particles disappeared,plastoglobulis became empty,and the membrane of chloroplast became disintegrated.

结果表明:随着盐碱浓度的增加,燕麦叶绿体膨胀,基粒片层扭曲、变形、松散,基质稀薄,淀粉粒消失,嗜锇颗粒变为空腔,膜逐渐解体。

With electron microscope techniques, the cytopathological changes of the tobacco leaves infected by TMV-W and TMV-O 17 were observed.

受TMV-017侵染的烟草细胞中,叶绿体数量较少,叶绿体具双层膜结构,基粒片层排列整齐,有的叶绿体膨大,内含少量小的淀粉粒。

By means of the electron microscopic enzymo cytochemistry, it was observed that the activity of acid phosphatase was localized on the autophagic vacuole, the enzyme reaction granules gather to form the vacuolar bodies of various shapes in the cytoplasmic region of the osmiophilic and paraglycogen granules in resting Stylonychia mytilus .

该文应用电镜酶细胞化学方法显示,贻贝棘尾虫休眠包囊中,在胞质嗜锇粒和拟糖原粒区,酸性磷酸酶反应颗粒聚集于具有消化泡作用的泡状体上,葡萄糖 6 磷酸酶反应也定位在自噬泡膜位置。

Previous study showed that over-expression of PICK1 in human leukemia REH cells rendered a higher resistance to etoposide-induced apoptosis through a recruitment of PKC alpha which resulted in maintenance of mitochondrial membrane potential, an enhanced phosphorylation of the anti-apoptotic Bcl-2 protein at Ser70, and inhibition of Bax dimerization.

在本实验室先前的研究,於人类血癌REH细胞中过度表现PICK1蛋白质,PICK1能够藉由招揽蛋白激酶C alpha至粒线体上,引起Bcl-2磷酸化程度提高,抑制Bax蛋白二聚体的形成,进而稳定粒线体的膜电位,以至於细胞对於化疗药物etoposide引起的程式凋亡具有抗药性。

A wheat hill drop planter used for perpendicularly inserting film mulching field was developed and its major shortcomings existed in the roller type planter were overcome.

引言滚轮式小麦覆膜穴播机存在的突出问题是种穴与膜孔错位率高,穴粒数均匀度差,易于造成空穴,成穴器撕膜、挑膜严重,种孔覆土不良。

Objective To acquire enough envelope glycoproteins so as to facilitate a further study of the structure and function of envelope glycoproteins from various kinds of virus isolates. Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV-1) proviral genome of HXB2 isolate.

目的 获得足够量的膜糖蛋白,以便于对不同HIV分离株膜糖蛋白的结构与功能进行进步的研究方法从人免疫缺陷病毒1(HIV-1)HXB2分离株原病毒基因组的重组质粒pHXB2中克隆了两段膜糖蛋白基因片段。

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