粒细胞

Methods: Eighty mice were randomly divided into high, middle, and low dosage groups which were orally given 1.5 g/kg, 0.75 g/kg, 0. 375 g/kg of ant powder respectively, twice a day for 10 days. A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组，黑蚂蚁粉给药量按小鼠体重1.5g/kg、0.75g/kg、0.375g/kg计算，连续10d灌胃给药后，测定各组小鼠的体重、胸腺和脾脏重量，计算胸脾指数；检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

Monocytes generated from bone marrow of Balb/cj mouse were cultured for 6 days in complete RPMI 1640 medium containing 10％ FBS, rmGM-CSF and rmIL-4.50 mg·L-1 acteoside or isoacteoside was added to cells on day 6 of culture for 24 h.The surface molecules expression level of DCs and their phagocytose ability were analysis by flow cytometry.

采用细胞因子诱导法，从Balb/cj小鼠骨髓细胞贴壁分离获得单核细胞，加入含10%胎牛血清、10 μg·L-1重组小鼠粒细胞巨噬细胞集落刺激因子及重组小鼠白细胞介素-4(rmIL-4)的RPMI 1640完全培养基培养，在培养第6天，实验组加入车前子苯乙醇苷类化合物(10，50，100 mg·L-1)，对照组加入RPMI 1640或LPS(1 mg·L-1)，流式细胞仪检测DCs表面分子CD11c，CD86，MHC II和CD80的表达以及各组DCs吞噬功能的变化。

A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组，黑蚂蚁粉给药量按小鼠体重1.5 g/kg、0.75 g/kg、0.375 g/kg计算，连续10 d灌胃给药后，测定各组小鼠的体重、胸腺和脾脏重量，计算胸脾指数；检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色：翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成；基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质，在翼状胬肉体部深层基质中存在粗糙的纤维组织；正常球结膜组织细胞排列整齐；基质为疏松结缔组织，胶原纤维平行排列，其间可见成纤维细胞，散在少量中性粒细胞、毛细血管；Masson染色：翼状胬肉浅层基质中存在致密的胶原纤维染色，深层基质中的胶原纤维存在变性样改变；VG染色：翼状胬肉组织深层基质中存在大量变性的胶原纤维，其间夹杂黑色的弹性纤维；免疫组化染色法：MMP-3在翼状胬肉上皮细胞中呈强表达，成纤维细胞中呈中等强度表达，血管内皮细胞中未见表达；LN在血管壁中呈强表达，在上皮细胞基底膜中呈中等强度表达，在整个基质中未见明显表达；col Ⅲ在整个翼状胬肉基质中呈强表达。

Objective To observe the influence of ChuanFuLing by applying on the points (Dingchuan\Felshu\Pishu) on children patients in the asthma remission phase, to research the mechanism of CFL and provide experimental basis for clinical preventing and treating asthma effectively.

目的 观察中药喘敷灵三伏穴位贴敷对缓解期哮喘儿童非特异性免疫球蛋白巨，嗜酸性粒细胞，白细胞介素习(IL-4)，干扰素γ浓度，体液免疫的影响，探讨中药喘敷灵穴位贴敷防治哮喘的疗效及其可能的作用机制，为临床用药提供理论依据。

ObjectiveTo observe the influence on IgE、 EOS、 IL-5、 IL-12 and clinical effect of ChuanFuLingTieJi by applying on the points (Dazhui 、 Dingchuan、 Feishu、 Pishu 、 Shenshu) of children patients with asthma,and compare with the ChuanFuLing-paste, researching the mecharism of CFLTJ and providing theoretical basis for feasibility of CFL form improvement and prevention and treatment of asthma.

通过观察中药喘敷灵贴剂三伏穴位(大椎、双侧定喘、双侧肺俞、双侧脾俞、双侧肾俞)敷贴对缓解期哮喘患儿临床症状、非特异性免疫球蛋白E、嗜酸性粒细胞、白细胞介素5(IL-5)、白细胞介素12(IL-12)的影响，并与疗效确切的喘敷灵传统剂型比较，探讨中药喘敷灵贴剂穴位敷贴防治缓解期小儿哮喘的临床疗效及其可能的作用机理，从而为中药喘敷灵剂型改良的可行性及其临床推广应用提供依据。

On the basis of the percentage of GPI-anchored PLAP conversion, the leucocyte GPI-PLD activities of the 96 leukemia patients were measured. Compared with the 96 healthy controls, the leukocyte GPI-PLD activites of ANLL and CLL patients were significantly increased; the activities of ANLL and CLL patients were significantly reduced.

检测96例白血病患者外周血白细胞GPI-PLD活性时发现，急性非淋巴细胞性白血病和慢性淋巴细胞性白血病患者酶活性较正常人显著性增高，急性淋巴细胞性白血病和慢性粒细胞性白血病患者酶活性较正常人显著性降低。

Results The study showed that immunoreactivity to SPR was present both on the surface and in the cytoplasm of epithelial cells, eosinophils, glandular cells and its ducts, mast cells, plasmocyte and other monocytes.

结果 SPR广泛分布于健康鼻粘膜上皮细胞、血管内皮细胞、腺细胞及导管、肥大细胞、嗜酸粒细胞、浆细胞和其它单核细胞。

The main pathomorphologic characteristics were pulmonary hemorrhage and pneumonedema.

光镜观察见肺间质毛细血管充血扩张，小动脉管腔狭窄，肺泡腔和间质明显水肿、出血，大量中性粒细胞浸润，肺泡内大量红细胞和浆液纤维渗出物。

While diagnosing and treating SARS cases, doctors should rule out the possibility of other lung diseases with similar clinical manifestations, such as primary bacterial or fungal pneumonia, pulmonary tuberculosis, lung tumor, non-infective interstitial lung diseases, pneumonedema, atelectasis, pulmonary embolism, pulmonary infiltration with eosinophilia, and pulmonary agents.

答：在非典型肺炎的诊断治疗过程中，首先要注意排除原发细菌性或真菌性肺炎、肺结核、肺部肿瘤、非感染性肺间质性疾病、肺水肿、肺不张、肺栓塞、肺嗜酸性粒细胞浸润症、肺血管炎等临床表现类似的肺部疾患。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。