粒层

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石。。。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

Under low temperature condition, fertility restoring, anther developing normally, the tetrads formed after archesporical cell, sporogenous cells and microspore mother cell. Subsequently, microspores were free from the tetrad of fertile anther and formed mature pollen grains, tapetumof the fertile anthers began to disintegrate and disappear at last.

在低温处理条件下，育性恢复，花药发育正常，经过孢原细胞期、造孢细胞期、花粉母细胞期后，可以顺利形成四分体，可育花药的四分体可以释放出小孢子并发育为成熟的花粉粒，同时绒毡层细胞开始解体，最后消失。

Their lithologic types are dominated by vitric tuff, vitric crystal tuff and medium-fine tuffy sandstone, and their genetic types mainly belong to complicated type of the airborne-water-carried and the airborne type.

火山事件层的岩石类型以玻屑沉凝灰岩、玻屑晶屑沉凝灰岩和凝灰质中细粒砂岩为主。火山事件沉积的成因类型以空降－水携复合型和空降型为主。

The problems for choosing crushed stones in making of carbon fiber conductive concrete for deicing or snow-melting are discussed.

讨论了将用于路面融雪化冰的碳纤维导电混凝土在制作过程中石子的选择问题，提出了在制作发热用导电混凝土铺层时，掺入石子应遵循的3个基本原则：石子的掺量不能太多；不能和标准的混凝土一样，采取合理的级配；掺入的碎石粒径不能太大。

The leaves from the plants treated with 0.1 mg·L~(-1) DBP showed that their chloroplasts further disintegrated,with their thylakoids and lamellar structure disorganizing,their cells appearing void and their organelles rarely found

0.1mg·L-1DBP处理后引起叶绿体的进一步解体，叶绿体中类囊体基粒和片层结构解体，细胞出现空洞现象，其它细胞器很少观察到

The wear invalidation forms of the WC/Cu-base alloy composite coating include the furrow on the surface of the coating because of the furrowing that abrasive particles to the Cu-base alloy matrix and the rupture and torn up of WC particles.

WC/Cu基复合层中组织细小、均匀的铜锌合金基体具有优良的塑性，并与硬质颗粒WC之间形成结合性能良好的界面，在磨粒磨损过程中，起到稳固硬质颗粒，吸收能量，减缓冲击的作用。

Chloroplast size was bigger and grana stack was thicker in tetraploid than that in diploid.

四倍体的叶绿体的长度和宽度均显著高于二倍体，基粒片层加厚，结构致密有序。

The colorless ground matter between the grana lamellae in a CHLOROPLAST.

基质：是叶绿体内的叶绿体基粒片层结构之间的无色透明物质。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。