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Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21(DE3). GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA,经逆转录-聚合酶链式反应扩增DOC-1的CDS序列,再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中,构建融合表达载体pGEX-4T-1-DOC-1,经酶切、测序鉴定后,用该重组质粒转化E.coliBL21,用IPTG诱导表达,Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

The test results show that the pH value of colloid solution is 7.80, the parti...

实验结果表明,获得胶体溶液的pH为7.80,镍/铜胶体的粒径随酒石酸钠钾摩尔浓度的增加而减小,镀层的剥离强度为1.38kg/cm。

The result shows that when the emulsifying agent layer not perfectly covering the primary particles' surface or the primary particles' surface being covered with overmuch powder grain, it will lead to the higher plastisol viscosity.

结果表明初级粒子表面的乳化剂层包覆不严或表层粉粒太多,均会导致增塑糊粘度偏高;次级粒子聚结松散,结构脆弱,有较低的糊粘度和触变性;次级粒子聚结紧密,有熔结现象,增塑糊粘度偏高。

Le Dracula is the main group for the ash, glaucum, purplish red basalt and andesite, fine-grained tuff, crystal-tuff debris, ash-tuff, tuffaceous siltstone, tuffaceous silty mudstone uneven beds, rocks folder jasper , Ansan porphyrite and long-sandstone and siltstone.

太勒古拉组主要为灰、灰绿、紫红色玄武岩、安山岩、细粒凝灰岩、晶屑沉凝灰岩、火山灰沉凝灰岩、凝灰质粉砂岩、凝灰质粉砂质泥岩不均匀互层,夹碧玉岩、安山玢岩及长石砂岩、粉砂岩。

Product characteristic: The utensil is natural dissolve enzyme formula, delivers effective matter to the skin inner urge a fat to eliminate secreta with natural row, deep of excessive sebum tier.

产品特点:具天然溶酶方子,将有效物质送到肌肤内层,促使油脂粒与过剩皮脂的自然排,深层断根分泌物。

The chloroplast ultrastructure of the sepal in the fresh-cut broccoli florets was investigated with a transmission electron microscope.

对鲜切西兰花叶绿体超微结构的研究表明,乙醇对叶绿体外膜及内部基粒片层结构均起到保护作用,减小了贮藏期间叶绿体的破坏程度,显著延缓了叶绿体结构的解体。

Investigation of sediment depth was made and the results show most of the sediments are deposited in the western shore-line area and Meiliang Bay. The laboratory results raise the possibility that there is significant difference in chemical or physical properities in the upper 10cm sediment, which suggests sediments are frequently resuspended in this layer.

通过对太湖底泥厚度的调查,发现太湖中底泥主要分布在西部沿岸和北部的梅梁湾;对底泥中各种理化特征垂直分布的分析表明,在表层5-10cm内,容重、粒径、含水率及有机质含量随深度的变化显著,说明表层5-10cm是底泥中频繁发生侵蚀-沉降-再侵蚀的活跃层。

The percentage of silt content was the highest(>32%) in each soil layers.

粉粒在各层土壤中所占的平均含量(>32%)最大。

Slope stabilization treatment of this region including polyathylen strip method and thin layer hydrograsser method, some small slopes even have not been treated. A few of the treated slopes fail during heavy rainfall. The post method of treatmenting these slopes obviously doesn't function properly. The purposes of this research is to suggest suitable methods for gravel slopes, and consider slope behavior and stability.

当初公路沿线砾石边坡於设计阶段时,采用了包括植生带铺植工法及薄层喷植工法二种护坡工法,另部份边坡高度较小之路段未予植生处理;由於此路段边坡易受雨水侵蚀而形成侵蚀沟,并使其细粒料流失,造成边坡植生效果不佳,所采用之护坡工法无法达到其预期之效果,本研究目的为建议适合之工法应用於现地砾石边坡上,并探讨边坡之整体行为与稳定性。

The result show, pH of solution, the type and concentration of surface-active agent all have marked effect, pH change can transform the disperse behavior of attapulgite particle by altering granularity distributing in different range, influencing double electromotive force between particle and solution.

研究表明,水溶液的pH值、表面活性剂的类型和浓度对凹土的分散稳定性皆有显著的影响,pH值的变化改变了不同粒径范围的粒子总量分布,改变粒子与溶液间双电层电动势,从而改变凹土粒子的分散行为:由于凹土作为带电粒子在极性溶剂中容易达到物理分散的目的,因此,凹土在极性溶剂中的分散状况大大优于在非极性溶剂中的分散。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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