粒化

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The function of hypothesis transport genes NMB0098 and NMB0097 were tried to study by way of gene knock out. The phototype of N. meningitidis strain BT878 was observed before and after gene NMB0098 was knocked out.

选用质粒pUC18为目的基因的载体，经过酶切、去磷酸化、连接、电穿孔法转导和抗生素筛选等步骤，分别将目的基因NMB0098和NMB0097与质粒相连，并在目的基因中插入标记基因—卡那霉素基因，构建盒式插入的重组质粒pNB0098-K和pNB0097-K。

The grain length, width and 100-grain weight of parents and 110 lines in waterland cultivation of were greater than that in dryland cultivation, and amylose content and chalkiness rate were less than that in dryland cultivation. The comparative study on RVA showed that the greater effect of water stress was observed on the cold plastic viscosity, consumers base value, the restoring value of RVA spectral characteristics and litter on gelatinization temperature, the peak time, the highest viscosity, hot plasma viscosity, disintegrating value.

在旱地种植亲本和各品系的粒长、粒宽和百粒重的均值均小于水田种植，而直链淀粉含量和垩白率均大于水田种植；稻米淀粉黏滞性特征谱中冷胶黏度、消碱值、回复值受土壤水分胁迫影响较大，土壤水分对糊化温度、峰值时间、最高黏度、热浆黏度、崩解值影响较小。

Germ-line mutations in the genes hTERT and hTR, encoding telomerase reverse transcriptase and telomerase RNA, respectively, cause autosomal dominant dyskeratosis congenita, a rare hereditary disorder associated with premature death from aplastic anemia and pulmonary fibrosis.

分别编码端粒末端转移酶反转录酶和端粒酶RNA的hTERT和hTR基因的种系突变导致常染色体显性先天性角化不良，它是一种罕见的因为再生障碍性贫血和肺纤维化而早亡的遗传性疾病。

Reflecting layer is composed of TiO2 large nano-particles with mean grain size of 250nm or 150nm prepared using organic alkali tetramethylammonium hydroxide as peptizer. Nano-crystalline TiO2 porous thin film composed of small nanocrystalline (10~20nm) was used as a substrate layer to absorb large amount of dye molecules.

用有机碱四甲基氢氧化铵做胶化剂制备的两种大粒径纳晶TiO2（粒径为250nm和150nm），用它们分别制备薄膜做为反射层；用小粒径纳晶TiO2(10~20nm)来制备的纳晶多孔薄膜为底层膜，用于吸收大量的染料。

It is caused by mutations in dyskerin-encoding genes, telomerase-encoding RNA component genes and reverse transcriptase genes, as well as other uncharacterized genes. There are three inherited forms, including X-linked, autosomal dominant and autosomal recessive inheritance.

先天性角化不良是与编码角化不良蛋白基因、编码端粒酶的RNA组份基因、编码端粒酶的逆转录酶基因突变及其他未确认的致病基因突变引起的基因病，其遗传方式有：X-性联隐性遗传、常染色体显性遗传及常染色体隐性遗传。

Chicken feed of model 661 is regarded as raw material of pellet feed, the influence that experimental factors including modulation-character temperature, modulation-character moisture, grease-additive quantity, and comminuting granularity have on making pellet quality, productivity, and electricity consumption. UG and ANSYS are regarded as simulating circumstance in finite element analysis, for problem on service life of the machine, evaluation index that average stress of a series of parameterized loop die is regarded as service life of hoop standard granulator is achieved by dynamic, static, and thermal structure coupling analysis for key part: loop die of the machine. For every influencing factor the experimental arrangement of array and combination is made by adopting optimizing idea and method of experimental design, the aim of quantification analysis is strived to arrive.

在试验设计中，采用正交试验的方法，以611型鸡饲料作为颗粒饲料的原料，分析混合饲料的调质温度、调质水分、油脂添加量、粉碎粒度等试验因素对制粒质量、生产率、电耗的影响；有限元分析中以UG、ANSYS作为仿真环境，针对制粒机使用寿命的问题，通过对环模制粒机中的关键零件环模进行动力、静力、热结构耦合分析，获得一系列参数化后的环模的平均应力作为环模使用寿命的评价指标；以实验设计的优化思想和有限元分析方法对各个影响因素作了排列组合式的实验安排，力求达到量化分析的目的。

Microanalyses have shown that during brazing Ti in AgCuTi alloy transferred preferentially to the surface of the cBN grit, and bonding behavior on cBN surface by AgCuTi alloy filler metal is realized through TiN and TiB2 which are produced by interaction between Ti atoms of AgCuTi alloy and N or B atoms of cBN surface. Thus, the chemical metallurgic bonding has been formed in the interface between AgCuTi alloy and c-BN grit.

微观分析表明：钎焊过程中AgCuTi合金钎料中的Ti向cBN磨粒表面富集，并与cBN磨粒表面N和B元素发生反应，形成TiN和TiB2，实现了AgCuTi活性钎料与立方氮化硼磨粒的化学冶金结合。

The plasmid pcDNA3.1-3-1E and pcDNA3.1 was respectively used to immunize SPF chicks by injection intra-muscularly in thigh muscle at 14 and 21 days of age.Chicks in each group except that in the unchallenged control group were challenged with 5×104 E.acervulina sporulated oocysts at 28 days post-inoculation,and the immune protective efficacy was observed.

将构建的重组质粒以及空载体质粒分别于14、21日龄分2次经腿部肌肉注射免疫SPF雏鸡，28日龄除非免疫非感染对照组外各组攻击性感染5×104个堆形艾美球虫孢子化卵囊，观察真核表达质粒对球虫感染鸡的免疫保护作用并探讨其免疫保护机理。

31DH suspend in the dry red wine and ferment,the density of the dry immobilized 31DH was changed by various materials.

通过选用不同的材料调节干固菌胶粒的比重，使固定化酒类酒球菌31DH悬浮于干红葡萄酒中进行苹果酸—乳酸发酵，并测定这些材料对固定化胶粒相对扩散系数的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。