粒化

Poptosis-inducing factor is a mitochondrion-localized flavoprotein with NADH oxidoreductase activity, which is encoded by the nuclear gene. Following induction of apoptosis, AIF is released from mitochondria and translocated to cytosol, and then to the nucleus. The mechanism of AIF translocation from mitochondria to nucleus is still unclear. It has been suggested that translocation is due to the presence of nuclear localization signal.

bstract： AIF(apoptosis-inducing factor)为位在粒线体内膜膜上之蛋白质，在正常细胞中，能够藉由维持电子传递链复合体I的稳定性，进而影响细胞能量的产生及自由基的清除，并且也会维持粒线体的构造；反之，当细胞遭受到不当刺激时，粒线体膜通透性改变，使得原本存在於粒腺体间质中之AIF，被释放出来，进入细胞核内造成DNA片段化，进一步促进细胞凋亡。

During the process of peptization and hydrothermal crystallization, the aggregation behavior and the resulting morphology of nanocrystalline titania (TiO2) were studied by X-ray diffraction, scanning electron microscope, and dynamic light scattering techniques.

通过XRD、SEM和动态光散射粒度测定仪研究了胶溶及水热晶化过程中纳米TiO2晶粒聚集行为及形貌。实验结果表明，在胶溶无定形沉淀过程中所形成的大小为10-15nm的锐钛矿型胶粒或金红石型胶粒易定向聚集成更大的、具有(30-50)nm×(80-100)nm的次级晶粒。含有次级晶粒的溶胶前驱液在水热晶化过程中，次级晶粒发生崩裂并生长成结晶度更高的大小约为10-30nm球形和大小约为20-60nm棒状纳米TiO2

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

The effects of the spray-drying process of the ammonium paratungstate and the carburization temperature on the formation of the precusor and WC powder were investigated.

采用喷雾干燥-固定床法制备碳化钨粉末，研究了喷雾干燥和碳化反应温度对制备偏钨酸铵前驱体以及碳化钨的影响，前驱体粉末呈现均匀的球状形态，平均粒径为10~20μm，喷雾温度对影响粒径大小和分布影响小；400℃的进口温度下进行喷雾干燥，喷雾粉末仍然保持偏钨酸铵原来的基本组成不发生分解。

In Salsola passerina -Reaumuria soongorica community, the first ordination axes explains the salinization gradient, along the order of Caragana tibetica community,Salsola passerina-Oxytropis aciphylla community,and Reaumuria soongorica-Salsola passerine community,soil alkalization increases. The second ordination axis explains soil structure gradient, along the order of Caragana tibetica community, Reaumuria soongorica-Salsola passerina community and Salsola passerina community, soil texture becomes coarser. In the Stipa breviflora-Stipa grandis community, the first ordination axis indicates the soil water gradient, and the second ordination axes explains hydrothermal coupling gradient. In the Prunus mongolica-Ulmus glaucescens community, the first ordination axis explains the soil pH gradient, along the order of Ulmus glaucescens-Prunus mongolica community, Prunus mongolica-Potentilla fruticosa community, and Potentilla fruticosa-Prunus mongolica community, soil pH value reduces. The second ordination axis explains soil structure gradient, along the order Prunus mongolica-Ulmus glaucescens community, and Prunus mongolica-Potentilla fruticosa community, the contents of silt and clay increase, and soil texture suggests a fine trend.

在珍珠猪毛菜-红砂群落，第一排序轴反映了土壤盐碱化梯度，沿着藏锦鸡儿群落—珍珠猪毛菜、猫头刺群落—珍珠猪毛菜、红砂群落序列，土壤盐碱化程度不断增强；第二排序轴则反映了土壤结构梯度，沿着藏锦鸡儿群落—珍珠猪毛菜、红砂群落—珍珠猪毛菜、猫头刺群落序列，土壤质地逐渐粗化；在短花针茅-大针茅群落，第一排序轴反映了土壤水分梯度，第二排序轴反映了海拔梯度上的水热组合梯度；在蒙古扁桃-灰榆群落，第一排序轴反映了土壤pH梯度，沿着灰榆、蒙古扁桃群落—蒙古扁桃、金露梅群落—蒙古扁桃群落序列，土壤pH值逐渐下降；第二排序轴主要反映了土壤结构梯度，沿着蒙古扁桃群落—灰榆、蒙古扁桃群落—蒙古扁桃、金露梅群落序列，土壤中粉粒、粘粒含量逐渐增加，土壤质地呈细化趋势。

In Salsola passerina-Reaumuria soongoriea community, the first ordination axes explains the salinization gradient. along the order of Caragana tibetica community, Salsola passerina-Oxytropis aciphylla community, and Reaumuria soongorica-Salsola passerine community, soil alkalization increases. The second ordination axis explains soil structure gradient, along the order of Caragana tibetica community, Reaumuria soongorica-Salsola passerina community and Salsola passerina community, soil texture becomes coarser. In the Stipa breviflora-Stipa grandis community, the first ordination axis indicates the soil water gradient, and the second ordination axes explains hydrothermal coupling gradient. In the Prunus mongolica-Ulmus glaucescens community, the first ordination axis explains the soil pH gradient, along the order of Ulmus glaucescens-Pnuius mongolica community, Prunus mongolica-Potentilla fruticosa community, and Potentilla fnuicosa-Prunus mongolica community, soil pH value reduces. The second ordination axis explains soil structure gradient, along the order Prunus mongolica-Ulmus glaucescens community, and Prunus mongolica-Potentilla fruticosa community, the contents of silt and clay increase, and soil texture suggests a fine trend.

在珍珠猪毛菜－红砂群落，第一排序轴反映了土壤盐碱化梯度，沿着藏锦鸡儿群落－珍珠猪毛菜、猫头刺群落－珍珠猪毛菜、红砂群落序列，土壤盐碱化程度不断增强；第二排序轴则反映了土壤结构梯度，沿着藏锦鸡儿群落－珍珠猪毛菜、红砂群落－珍珠猪毛菜、猫头刺群落序列，土壤质地逐渐粗化；在短花针茅－大针茅群落，第一排序轴反映了土壤水分梯度，第二排序轴反映了海拔梯度上的水热组合梯度；在蒙古扁桃－灰榆群落，第一排序轴反映了土壤pH梯度，沿着灰榆、蒙古扁桃群落－蒙古扁桃、金露梅群落－蒙古扁桃群落序列，土壤pH值逐渐下降；第二排序轴主要反映了土壤结构梯度，沿着蒙古扁桃群落－灰榆、蒙古扁桃群落－蒙古扁桃、金露梅群落序列，土壤中粉粒、粘粒含量逐渐增加，土壤质地呈细化趋势。

Effect of protein and lipid in rice on starch physicochemical characteristics, difference between the region of non-chalkiness and chalkiness of rice, dynamic variation of cell structure and CaM distribution on rice spike were expounded in this study. It was found that protein and lipid in rice had important effect on starch gelatinization, there were distinct differences between the region of non-chalkiness and chalkiness of rice, vascular bundle system in rice spike was changed during grain filling, difference quality of different position of rice spike was more distinct in dense spike rice for more powerful vascular bundle system, the development of rice seeds was corresponded to pericarp development, difference quality of different position of rice spike was also related to transport function of rachilla besides activity of "source", and calmodulin relative activity was closely related to grain filling velocity and enginery properties.

通过实验发现：稻米中蛋白质、脂肪对淀粉的糊化过程有着较大的影响；精米的非垩白与垩白部位以及心白与腹白部位之间在蛋白质、氨基酸含量及组成方面存在着明显差异：水稻穗部大维管束面积以及主穗轴维管束数目在灌浆过程中存在明显的动态变化趋势；密穗型水稻强势粒的维管组织比散穗型水稻发达，致使其强、弱势粒维管组织差异明显加大；果皮的发育与籽粒发育状况是相适应的：强势粒和弱势粒在灌浆生理方面的差异，不仅表现在籽粒本身的&库活性&上，而且也与其着生小穗轴的输导功能有关；CaM相对活性与水稻灌浆速率、机能特征间存在着密切联系。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

OLH or 8-bromo-cAMP were added to luteinized granulosa cells. Results indicated that, protein expression of HIF-1a, HIF-1b and VEGF had a better performance in hypoxia than in normoxic condition, but phospho-PKA, StAR and P450scc protein expression had weaker expression in the former condition than in the later one. As a result of decreasing secretion of progesterone the decreasing of phospho-PKA was suspected to have a role in the reduction of StAR and P450scc in the condition of hypoxia.

结果发现，黄体化粒性细胞於缺氧条件下其HIF-1a、HIF-1b与VEGF蛋白质表现量皆较正常氧高；同时，StAR及P450scc蛋白质表现量则降低，但3b-HSD不受缺氧影响；此外，藉由特异性抗体检测PKA讯息传递路径发现，PKA讯息传递路经於缺氧条件下磷酸化PKA（phospho-protein kinase A）蛋白质表现量下降，可能经此路径的调控造成StAR及P450scc蛋白质表现量下降，导致黄体化粒性细胞孕酮分泌量减少。

During the processes of conditioning and pelleting, decrease of particle...

调质和制粒过程中，小粒度有利于蛋白质变性和淀粉糊化，使蛋白质体外消化率和淀粉糊化度提高；增加水分有利于淀粉糊化，而对蛋白质变性、VC活性减小和有害微生物灭活的影响较小；升高温度有利于蛋白质变性和淀粉糊化，使VC活性减小和有害微生物灭活；在高温调质和制粒过程中，饲料中的绝大部分致病菌[0]可被杀灭。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。