粒化

Both methods combined the magnetic microspheres technology with hydrazide chemistry and Lectin, respectively. The hydrazide-functionized magnetic microspheres and GNA-functionized magnetic microspheres are prepared correspondingly. The condition for capturing glycopeptides by hydrazide-functionized magnetic microspheres and immobiling GNA onto the magnetic microspheres was evaluated and optimized, and the specificity and validity of two methods was demonstrated by using standard glycoproteins sample.

试验中分别将肼化学和凝集素与磁性微粒相结合，分别制备出了肼功能化磁粒和GNA功能化磁粒，优化了肼功能化磁粒富集糖肽的条件和GNA固定的条件，并通过标准蛋白样品对两种方法的富集特异性和有效性进行了验证。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The effect of dosage of creaming reagent on creaming time, latex solid content and average particle size was studied, and it was found that the composite latex particle size was too large, and the concentration period was too long, about 15 days.

以海藻酸钠为膏化剂对复合胶乳进行了膏化浓缩，研究了膏化剂用量对膏化时间、胶乳固含量和胶乳粒径的影响，结果表明膏化浓缩胶乳粒径较大，膏化周期较长，一般需半个月左右才能得到实用的浓缩胶乳。

The fly ash, granulated blast furnace slag and natural zeolite powder are the mineral additions for concrete mixing.

条目备注：粉煤灰、粒化高炉矿渣粉和天然沸石粉是拌制混凝土的矿物掺合料。

The main ways to solve these problems are as follow: 1 to mix steel slag with cement clinker, grained iron slag and fly ash to improve the activity of mixture and the intensity of steel slag cement ; 2 to add suitable excited agent, such as calcium sulphate and sodium silicate.

根据对大掺量钢渣水泥的组分活性差和强度低的主要影响因素及其解决方法进行试验的结果认为：可以通过掺加水泥熟料、粒化高炉渣和粉煤灰的方法来改善钢渣的物质组成，进而提高其强度，但必须加入适当的激发剂。

After heat treatment, borides of samples made of sand mould were changed little, otherwise, borides of samples made of metal mould were changed to granulation shape and the impact toughness was increased by 60%..

高硼铁基合金熔体在金属模和砂模不同凝固条件时，奥氏体初晶晶粒随冷却速度增加而减小，硼化物形态发生较大变化；热处理后，砂模铸造的试样，硼化物形态变化不大，而金属模铸造的试样硼化物粒化，其冲击韧度提高了60％。

At present, dealing with the apparatus containing oily water has much kind, mainly nature except that oil tank, swash plate except that oil tank, thick grain take except that oil tank, pressure except that oil tank, make up, degrease device, pressure strain pot and single valve strain pot,etc..

目前，处理含油污水的设备有多种，主要包括自然除油罐、斜板除油罐、粗粒化除油罐、压力除油罐、组合除油装置、压力滤罐和单阀滤罐等。

The slag with different amount of TiO2 were synthesized and prepared in laboratory and the effect of the amount of TiO2 in slag on cement properties was studied.

通过实验室合成不同钛含量的粒化高炉矿渣，研究了矿渣中钛含量对矿渣水泥性能的影响。

Pelletizing is an increasingly important process for fine grain ore concentrates.

粒化是精铁矿的重要过程。

This pre-treatment of ore concentrate is a vital step for downstream pelletizing in particular where bentonite and /or other additives are intermixed, and where liquid components are added.

精铁矿的预处理是接下来粒化的重要阶段，特别是彭润土和/或者其他添加剂的混合，其中有液体成分的添加。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。