粒化

The function of proliferation and phosphorylation in vitro or in vivo was further studied.

利用基因重组的方法构建该基因的表达质粒，并研究该基因在细胞水平和活体水平对细胞增殖度磷酸化功能的影响。

In the biotechnology area, we has set up polytype Handson yeast express system which owned freedom intellectual property rights, including:(1) URA3 - defective nutrition host cell strain HU-II;(2) secretion and intracellylar expressing plasmid carrier pMPT-01、pMPT-02;(3) a platform for semi-works

在生物技术领域：建立了拥有自主知识产权、较为完善的多型汉逊酵母表达系统，包括：（1）URA3 -营养缺陷型宿主细胞株HU-II；（2）分泌型和胞内型表达质粒载体pMPT-01、pMPT-02；（3）汉逊酵母细胞电穿孔转化操作程序及多拷贝、稳定化整合转化子筛选程序；（4）中试生产规模的发酵和纯化平台。

The powdering cause of sinters is analysed and improving measure is put forward in this paper.

烧结矿小粒级偏多一直是困扰南钢烧结生产及高炉生产的难题，本文对烧结矿粉化的原因进行了分析并提出了改善措施，对生产实践进行了总结。

Recombinant expression vector k14/c-myc-paav was constructed bearing the mouse c-Myc cDNA derived by the skin specific human keratin 14 promoter, then the linear vector was microinjected into the two pronucleus of the C57BL/6 fertilized eggs to produce transgenic mice.256 microinjected eggs were transferred, 6 of the foster mothers were pregnant and 44 adult mice were produced, two of them were positive transgenic mice confirmed by PCR and Southern blot analysis, transgenic rate was about 4%.

以CMV-PAAV载体为基础载体，以皮肤特异人K14（人角蛋白14）基因启动子替换原质粒中的CMV启动子后，在其多克隆位点区依次插入鼠c-Myc基因的cDNA序列和gfp基因的编码序列，构建了高效表达c-Myc基因的真核表达载体。通过双原核显微注射技术，将线性化的载体DNA注入到C57BL/6小鼠受精卵的两个原核中，然后将注射后状态良好的胚胎移植到代孕鼠的输卵管中，共移植了256枚受精卵，6只受体怀孕，最终获得44只成年小鼠。

The results show that the ζ potential of PYM-PO4SiNP decreased obviously, and the release rate of PYM from the PYM-PO4SiNP accelerated with increase of added THPMP. However, the quantum of THPMP had no impact on the size of PYM-PO4SiNP.

结果表明，随着磷酸化修饰试剂量的增加，制备的PYM-PO4SiNP的电位逐渐降低，其包载的PYM的释放速率逐渐加快，但对颗粒的粒径没有明显影响。

Producted RF and CRF aerogels by process of sol-gel technics, dynamic-exchange, CO2 supercritical drying, and procedural increase in temperature carbonization methods, which have eximious capability and nanometer network structure; Systemic analysis various factors which effect gel course, such as ratio of reactants and the dosage of catalyst, and probed into the better experiments conditions(the concentration of resorcinol and formaldehyde is more than 2% and the value of R/C is between 50 and 300); During gel course , primary investigated the viscosity curve and particle size distribution of sol-solution, and analyzed the reason of catalyst dosage effects particle size of gel, and confirmed that the up-growth of gel follows the theory of " polymerize increase ".The experiment results indicated that ultrasonic with unvarying temperature disposal shortened gel time and optimized quality of gels, and the factors of ultrasonic effect gel reaction velocity were analyzed; Prepared organic aerogels using compounds of resorcin and hydroquinone substitute resorcinol reacts with formaldehyde the first time, and also optimized the proportion of resorcinol and hydroquinone (the proportion of hydroquinone in 10%～40%).

采用溶胶-凝胶工艺结合动态溶剂交换、CO2超临界流体干燥与程序升温碳化方法制备出性能优良、具有纳米网络结构的RF与CRF气凝胶；深入研究了反应物配比催化剂用量等条件对凝胶过程的影响，得到了较佳的RF凝胶制备条件(RF浓度应大于2%，R/C值取50～300)；初步研究了凝胶过程中的粘度、粒径变化情况，分析了催化剂用量影响凝胶中粒子尺度的原因，在实验上印证了凝胶生长遵循"聚增长"的理论；通过引入超声波改进了RF气凝胶的制备工艺，缩短了凝胶反应时间，提高了凝胶品质，并探讨了超声波促进RF凝胶反应作用的原因；首次用对苯二酚与间苯二酚复配制备了混合有机气凝胶，优化出复配比(对苯二酚含量为10%～40%)。2。

The α-AE_(75) cDNA was fused downstream of melC1 signal peptide encoding sequence in correct reading frame so that α-AE_(75) will be expressed secretively.

用这两个表达质粒分别转化S.lividans得到能分泌表达α-酰胺化酶的工程菌S.lividans [pIJmel／AE680]和S.lividans[pMS-mel／AE680]。

The 5＇coding region of its eDNA was optimized according to the codon bias of Pichia pastoris to make it secretively express in Pichia pastoris with higher performance. Two-step DNA synthsis was used to synthesize the eDNA sequence being optimized of ADTZ. OPT-ADTZ was connected with constitutive plasmid pGAPZaA to construct the reecombinant plasmid pNOA. pNOA was linearized and then transformed into Pichia pastoris GS115. Then codonoptimized ADTZ was constitutively and secretively expressed in Pichia pastoris.

为了使重组蛋白rADTZ能在毕氏酵母中高效分泌表达，根据毕氏酵母密码子的偏好性对rADTZ的5'末端的编码区域进行了优化，利用two-stepDNAsynthsis技术合成出ADTZ优化的基因序列OPT-ADTZ，并与组成型表达载体pGAPZaA连接，构建重组质粒pNOA，线性化pNOA后转化至毕氏酵母GS115中，实现了密码子优化的rADTZ组成型分泌表达。

Its structure is characterized by the fact that the sieve plate is replaced by a bubble cap plate and a plate of large holes to make water distribute better;a special shroud.

本文研究的防堵防跑双罩式内循环流态化水冷器的结构特点是：底室采用泡罩板加大孔均布板取代筛孔容易堵塞的筛板；水冷管出口安装挡粒调匀罩。

Dongan gold deposit is a epithermal gold deposit occurring in the strong silicification zone of Late Indosinian alkali feldspar granite and Late Yanshanian fine-grained alkali feldspar granite in the intermediate-acidic volcanic-intrusive rock.

东安金矿床是浅成低温热液型金矿床，赋存于中酸性火山-侵入岩、印支晚期碱长花岗岩和燕山晚期细粒碱长花岗岩脉强硅化蚀变带中，受库尔滨断裂的次级断裂控制。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。