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The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.

通过双向凝胶电泳分别分离TGIF转染细胞和PcDNA3.1空白质转染细胞的总蛋白质,测得两组细胞的蛋白质斑点数分别为760±41和680±38个;位置重复性分析表明TGIF转染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm,在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm;PcDNA3.1空白质转染细胞在IEF方向上的平均偏差为0.843±0.115mm,在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。

Plackett-Burman experimental design and the uniform experimental design were used to select the main factors of the ball milling pretreatment of corn stalk. The experimental results were analyzed by data processing software and well-fitted regression equation was established. Effects of ball milling parameters, such as raw material diameter, speed, ratio of raw materials, grinding media and alternating time, on the enzymolysis were analyzed. The optimal parameters of ball milling pretreatment of the corn stalk were as follows: particle size of raw material of 0.5 mm, speed of 340 r/min, the raw material content of 3.4 g, the number of ball of 15 (Ф= 10.0 mm), alternating running time of 5 min.

研究了玉米秸秆水解过程中球磨预处理工艺的优化,采用Plackett- Burman 试验设计和均匀试验设计法,用数据处理软件对试验结果进行分析,筛选出了球磨预处理过程中的主要影响因素,得到了具有较好拟合度的回归方程;通过分析原料径,转速,原料填装量,研磨介质,交替时间等球磨条件对酶解效率的影响,得出行星式球磨机粉碎玉米秸秆的最佳工艺参数为:原料径0.5 mm,转速340 r/min,原料填装量3.4 g,装球量15个(Ф=10.0 mm),交替运行时间5 min。

After addition of surface active agent into sprinkling solution, for particle of 1 μm size, the filtration rate has increased from 40% to 70% approximately under the condition of head wind velocity of 1.5 m/s and spr4nkling density of 2.36 kg/(m^2s), and the filtration rate has increased from 30% to 60% approximately under the condition of head wind velocity of 2.8 m/s and sprinkling density of 2.36 kg/(m^2s).

在喷淋溶液中添加表面活性剂后,对于径为1 μm的尘,在迎面风速为1.5 m/s、淋水密度为2.36 kg/的条件下,过滤效率由40%左右上升至70%左右。在迎面风速为2.8 m/s、淋水密度为2.36 kg/条件下,过滤效率由30%左右上升至60%左右。

The testing results of particle classifier using MATLAB tool cabinet, indicated that recognition ability of the classifier is 90%, and which can highly valued in application.

利用 MATLAB 工具箱对磨分类器的测试实验结果表明,磨分类器识别能力为 90%,具有比较高的实用价值。

The results indicated that rows per ear, kernels per row, ear length, ear diameter, kernel length, 100-kernel weight and stem diameter reduced gradually; barren tip, plant height and ear height increased gradually; shelling percentage and yield increased first and then decreased; while volume weight showed decreasing first and then increasing.

结果表明:随密度提高行数、行数、穗长、穗粗、籽长、百重和茎粗逐渐减小;秃尖长、株高和穗位逐渐增加;出籽率和产量先增加后减小;容重先减小后增加。

Just started a three-day fever, the highest 39.1 degrees, was later linked to the hospital for three days of water, the fourth day of diarrhea and vomiting since the afternoon, go to the hospital linked to water, hanging by nearly a week见好not actually check the stool also found fungal infection, immediately after the cessation of linked water, to do massage with the enema, it is not diarrhea, but is still very hard stools, a one, may I ask how can we rule fungal infection?

宝宝前阵子拉肚子,挂了好几天的水,抗生素用多了,霉菌感染怎么办?刚开始三天发烧,最高39.1度,后来去医院挂了三天的水,第四天下午开始腹泻呕吐,去医院挂水,挂了近一个礼拜都不见好,居然还检查大便后发现霉菌感染,后马上停止挂水,改做推拿跟灌肠,现不腹泻,但大便还是很硬,一,请问怎么才能治霉菌感染?

After the animal modle done successfully, all animals were randomly divided into two groups. group l: Microbubble contrast agent containing plasmid was injected into nude mice via the tail vein; group 2: the mixture of microbubbles with plasmid was injected as the group 1, and eyeballs were exposed to ultrasound with intensity of 0.5W/cm^2 immediately, and the time were all 60s that working time control at 20%.

将12只BAlB/C裸小鼠双眼玻璃体腔接种HXO-Rb44细胞,造模成功后,将动物随机分为2组,第1组于尾静脉注入含质的微泡造影剂;第2组尾静脉注射质与微泡的混合液,并立即以0.5W/平方公分〔1〕的超声波辐照小鼠眼球60s,工作时间控制为20%。

To the gradations with the same maximum nominal grain size,grey correlation was used to analyze the results. The conclusion showed that,except the 9.5 mm-maimum-nominal-grain-size gradation,Jf was influenced mostly by the subtotal triage of coarse aggregates in the third large sieve pore.

对于颗最大标称径相同的级配,使用灰色关联分析,得出结论:除颗最大标称径为9.5 mm级配外,Jf受级配中第3大筛孔分计筛余百分率影响最大。

In this study,additives were used to con-trol the size and shape of CaCO3.Needle,sphere,short chain and hexangular plate likenanometer CaCO3were synthesized by introducing CO2to lime suspension under opti-mum condition.

采用鼓泡碳化法,在最佳反应温度、反应物浓度、气体流量的条件下,考察了不同添加剂对产物形状及径大小的影响,得到了径小于100 nm且分散性能良好的球状、针状、短链状及六角板状纳米碳酸钙。

The results showed that the improved of 100-seed weight was limited by plant height,seed number per pod,number of main stem and hight of pod-setting.However,the i...

在大豆杂交种的选育过程中,在保持一定单株荚数的基础上,有针对性地选择荚大、大、重的个体,并注重品质性状,有望培育出优质、高产的大豆新品种。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。