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The carboxyl-terminal fragment of approximately 15ku of zonula occludens toxin gene encoding the 264-399 amino acid residue was amplified by PCR and then cloned into the prokaryotic expression plasmids PBCX.

用PCR扩增了MBP-ZOT质上的ZOT基因,该基因编码264~399位氨基酸残基C末端的15ku ΔG片段,并与经改造后的质PBCX连接。

When it comes to buying staples like rice, stay with the basic wholegrain or long grain rice and avoid buying expensive fragrant rice or branded items.

当它来购买斯台普斯像大米、与基本全或长谷大米和避免购买昂贵的芬芳的米饭或品牌的物品。

Purging influences the Kefir grains greatly, the vril of the Kefir grains can only be the vril we need under the continuative culture for 16 generations (pH3.75) even though we purge it every other generation. Under the same technological condition, we draw such a conclusion, so it is of more practical significance for us to use the method of non-purging continuative subculture.

清洗对开菲尔的影响极大,既使采用隔代清洗开菲尔的活力也需连续培养十六代才能达到所需活力(pH值3.75),在工艺条件完全相同的情况下得到这一结论,显然采用不清洗连续传代更具有实际意义。

The infectivity of TMV recovered by dialysis from incubation with anthraquinone glycosides in vitro has been reduced in contrast to infectivity of TMV control, it seems that anthraquinone glycosides have direct viricidal effect on TMV. The anthraquinone glycosides may interfere recognization of TMV to host receptor when applied before TMV inoculation. The anthraquinone glycosides can inhibit multiplication of TMV in infected leaf discs but did not inhibit infectivity of TMV-RNA in vitro. The control of tobacco mosaic virus disease of N.

总蒽醌甙对TMV混合,对体形态无破坏作用,但经透析,其抑制作用仍较高,表明总蒽醌甙与TMV结合能力强不易解离或对体具有直接毒害作用;总蒽醌甙预先接种可能影响病毒对侵染位点的识别,对叶圆片中的病毒具有抑制复制的作用,但在体外混合不影响TMV-RNA的侵染活性,可能与总蒽醌甙干扰病毒的衣壳装配过程或影响复制酶的活性有关。

He results show that the virus regained infectivity, and the tannins did not direct viricidal effect. Tannins extracted from Polygonum perfoliatum or Archontophoenix alexandrae seeds showed strongly anti-TMV activities separately. TMV virions seems to be aggregated when TMV incubated with the tannins by electron microscope. It seems that TMV coat protein bind to the tannins based on PAGE. Tannins did not reduce infectivity when applied either before or after TMV inoculation. It is proposed that tannins extracted from the 11 species inhibited TMV by binding with TMV coat protein to interfere with recognition site essential for viral coat protein. Tannins extracted from Euphorbia hirta, P. perfoliatum, P. cuspidatum reduce infectivity when applied immediately in N.

MV分别与这11种植物单宁混合后,通过透析除去单宁后,可恢复大部分侵染力,表明单宁对病毒体并无直接毒害作用;TMV与杠板归和假槟榔种子中提取的单宁分别混合后,电镜下观察体呈现明显的聚集现象;TMV外壳蛋白分别与这两种植物单宁混合后,聚丙烯酰胺凝胶电泳中CP泳带滞后;单宁在病毒接种前施用未降低TMV对叶片的侵染能力;这些结果表明植物单宁对TMV抑制作用可能是单宁与TMV-CP结合影响病毒对叶片侵染位点的识别而降低TMV侵染性,而这种结合是可逆的。

This machine is suitable for the grinding and arranging of the dry and block materials by the lines of pharmacy , chemical ,and foodstuff , as well as for the re-arranging of unqualified troches .

本机适用于制药、化工、食品等行业干燥的块状原料的粉碎和整,也适用于不合要求药片的重新整

They can be moved with soil particles and used as tracers for erosion and/or accumulation of soil particles.

它们在表土的迁移过程中具微迁移性,能较好地随土般运,可作为土侵蚀或沉积的示踪剂。

"We analyse soils according to the size of the particles, and the size of the particles determines to a great degree the different tillages, crop rotation and different crops to be grown on the soil."

我们根据土的大小来分析土壤,而且,土的大小在很大程度上决定着土地的耕作方式、农作物的轮作和种植的作物的种类。

The results showed that the order of the effects of vary traits on the yield was lodging resistance, ear height, ear yield rate, plant height, rows per ear, thousand kernel weight, growth period, ear length, ear thicks, weight per ear, kernel per row.

结果表明,湖北省玉米种植区玉米杂交种产量与主要农艺性状的关联度大小排序为抗倒性>穗位高>出子率>株高>穗行数>千重>生育期>穗长>穗粗>单穗重>行数,与产量密切相关的是品种的抗倒性、穗位高、出子率、株高、穗行数。

Objective:To study the changes of telomerase activity and telomere length.

目的:探讨肾癌组织端酶活性和端长度变化。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。