粒

High concentration white masterbatch is prepared by using different titanium dioxide, PE wax and adding nanocalcium carbonate etc. to change the formulation of masterbatch in this paper. Hiding power, tinctorial power and dispersion performance were tested by color measure instrument and IBAS, and process performance, rheology were tested by Brabender torsion rheometer and INSTRON rheometer.

本文采用不同的钛白粉、聚乙烯蜡及添加纳米碳酸钙等助剂的方法改变浓色母粒的配方，加工制备浓白色母粒，通过测色仪、图像分析仪测试母粒的着色力、遮盖力和分散性能，通过转矩流变仪和INSTRON流变仪研究了母粒的加工性能和静态流变性能。

Methods Telomerase activitiy in cytoplasmic and nuclear extracts of HCC samples was analyzed by an unamplified telomerase assay based on the radioactive incorporation and liquid scintillation counting and silver|staining TRAP（telomeric repeat amplification protocol）.

海医系防化医学教研室目的比较肝癌组织细胞质和细胞核中端粒酶活性。方法以端粒酶延伸产物液体闪烁计数法和端粒重复序列扩增法分别检测肝癌组织细胞质与细胞核中端粒酶活性。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Based on the investigation on the various results of the race YUNCAN 7 and YUNCAN 8 and analysis on the result items, the found shown the effect of cocoon number per kg on the some items of cocoon production and egg production, that was negative relation between cocoon number per kg and cocoon production per g of new hatched larvae, egg production per g of new hatched larvae, and hatch－ing percentage; positive relation between cocoon number per kg and egg number per g and no effect on other items.

对云南省桑蚕普种场2005年春季不同生产小组饲育的云蚕7、云蚕8蚕品种各项成绩进行了综合调查，通过对其调查的有关项目数据进行分析总结，发现蚕茧成绩中的&kg茧粒数&对蚕茧成绩中和蚕种成绩中的部分项目产生了影响，即：&kg茧粒数&与&g蚁收茧量、g蚁制种量、孵化率&呈负相关性，&kg茧粒数&与&g卵粒数&则呈正相关性。而对其它一些项目则无影响。

Telomerase activation is a key tumor marker which immortalizes cell.If a medicine blocks telomere prolonging by attenuation of telomerase activity, it could inhibit tumor cells proliferation.

因为端粒酶活化是大多数肿瘤细胞维持端粒长度并获得永生化的必要条件，如果某种药物能抑制端粒酶的活性就可阻断端粒的合成，从而使细胞退出增殖周期，达到抑制肿瘤细胞增殖的目的。

The results indicate that ① the reactor with sulfur particle size of 2.8-5.6 mm can effectively remove nitrate from nitrified leachate up to a concentration of 400 mg/L NO - 3 N at a hydraulic retention time of 5.71h;② the minimum hydraulic retention time necessary for complete denitrification depends on sulfur particle size and influent nitrate concentration;③the maximum volume...

结果表明，当HRT为5.71h，硫磺粒径为2.8－5.6mm时，最高处理浓度（以NO－3－N计）达400mg／L。达到完全反硝化所需的最小停留时间取决于硫磺粒径及进水硝酸盐浓度。硫磺粒径为2.8－5.6mm，5.6－11.2mm，11.2－16mm时，NO－3－N最高容积负荷分别为594，278，175.4g／（m3d）。NO－3－N最大表面负荷约为0.68g／（m2d），是自养反硝化的控制因素，与硫磺粒径无关。

Results: The rate of abnormal increase of telomerase activity was 75.71%, pos itive rate of telomerase was 31.41%,OD value was 388.21(×10-3) in hypertension group（70 cases） were significantly increase (P＜0.01,＜0.0 5,＜0.01),comp ared with 47 cases' normal group(RAITA was 31.79%,PRT was 6.38%,OD value was 7 0.7).The difference of RAITA was not significance (P＞0.05) between aged h ypertension group (73.68%) and normal group(52.63%),the difference of RAITA w as significance (P＜0.05) between adult(age＜60) hypertension group (7 5.00%) and normal group(17.81%). The difference of RAITA、PRT was no signific ance among 2 groups of hypertension, and was significanceP＜0.05）among 2 g roups of normal.

结果：高血压病组（70例）端粒酶活性异常增高率（75.71%），阳性率（31.41%），OD值［388.21(×10-3)］分别明显高于正常组（47例）的 31.79%、 6.38%、70.7( P＜0.01，＜0.05，＜0.01)；老年高血压组的端粒酶活性异常增高率(73.68%)与正常同年龄组的52.63%比无明显差异，但成年（＜60岁）高血压组的端粒酶活性异常增高率(75.00%)较正常组的17.81%明显增加(P＜0.05)；高血压2个年龄组的端粒酶活性异常增高率、阳性率、OD值无明显差异，但正常人2年龄组这些参数有明显差异（P＜0.05）。

With increasing the concentration of the mixture of antimony oxide from 10 % to 20 %, the reaction time decreases from 90 min to about 30 min, but the optimized range of molar ratio of H3PO4 to antimony increases. The reaction temperature is not the main factor on particle size with the existence of H3PO4, the average particle size is c.a. 160nm without obviously variety in the temperature range from 60 °C to 90°C.

氧化锑混合物浓度从10%增加到20%时，反应达到平衡的时间从90min下降到30min左右，形成小粒径胶粒所需磷酸与锑摩尔比的最佳范围也随之上升；在稳定剂磷酸存在的条件下，温度不再是对胶体粒子粒径的主要影响因素，在60～90℃之间，形成的胶体五氧化二锑的粒径无明显变化，在160nm左右。

Thirty six homologous Pairs of chromosomes were autosomes, in which chromosomes, Pairs of 1 to 8 were metacentric.9 to 36 . And X was metacetric, Y chromosome terminal.

其中，1—8对常染色体为中间着丝粒染色体，9-36对常染色体为端部着丝粒染色体，X为中着丝粒染色体，Y为最小的为端部着丝粒染色体。

The reservoir rock types in the region of interest are grain limestone, algal limestone, cryptite, siltstone, muddy limestone and mudstone. The thesis identified as a result of changes in the deposition causing the heterogeneity in plane, and then the geological oil reserves have been calculated in accordance with the areal heterogeneity.3、Based on the data of core analysis, bore logging, array induction imaging log and FMI, porosity and permeability model were built using regression analysis. Through testing 45 rock samples, a, b, m, n value were obtained and used to calculate single-layer oil saturation value using the Archie formula. According to the theory of laterolog and inductolog, resistivity ratio and cross plot were used to identification effective thickness. Effective thickness was identified delicately in 103 wells. Based on the over work, active porosity and oil saturation was calculated accurately.4、According to observation of 100 pieces of the thin slices, cast thin slices and 383m core in three wells, the chief diagenesis in this area were compaction, cementation, denudation and cataclasis. Cementation and denudation were universally influenced active porosity this area. The intergranular pores, residual pores and secondary pores were formed in thin carbonated reservoir of lacustrine facies.5、The influence of reservoir heterogeneity to reservoir calculation was analyzed systematically using actual data. And the conclusions were as follows:①The principal reason leading to oil distribution unequal is interior structural feature of reservoir in structural hydrocarbon reservoir.

确定了由于沉积微相的变化而导致的平面非均质性，进而在平面上按照平面非均质单元计算了石油地质储量。3、利用岩心分析、常规测井曲线与阵列感应、成像测井资料相结合，分岩性利用回归分析方法建立了储层孔隙度、渗透率模型；通过对45块岩样进行岩电试验，获取了a、b、m、n值，利用阿尔奇公式计算了各单层含油饱和度值；根据侧向电阻率和感应电阻率串并联特性，利用电阻率比值法与常规交会图法相结合重建了有效厚度图版，对全区103口井目的层段进行了有效厚度精细划分，通过以上工作，准确求取了储量计算单元内有效厚度参数、有效孔隙度参数以及含油饱和度参数。4、通过对研究区3口井383m岩心观察，100余块薄片、铸体薄片镜下鉴定得出：油泉子油田上、下油砂山组储层主要成岩作用是压实作用、胶结作用、溶蚀作用和破裂作用，其中溶蚀作用、胶结作用最普遍，这两种作用对有效孔隙度影响最大，以上成岩作用形成了原生粒间孔、粒间残余孔和次生孔隙(如粒间溶孔、粒内溶孔、铸模孔、晶间孔等)为主的薄层湖相碳酸盐岩储层。5、利用实际资料，系统分析了油藏非均质对储量计算的影响，认为：①对于构造油气藏而言，油气藏构造及断裂特征是造成油气分布不均的首要原因，储集条件相同的条件下，构造高部位有利于储存油气，油气在垂向的重力分异体现了油气藏内部构造的不均一特征。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。