粒

Full length LMP2A cDNA was firstly incised from pGEM-T-LMP2A with EcoR Ⅰ, Sma Ⅰ digestion, and then inserted into eucaryote. expression plasmid pCIcc controlled by CMV promoter. The CMV-LMP2A-SV40 expression unit was digested by ClaI, and inserted into E1-substituted adenovirus vector pAx1cw. Then the LMP2A recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I digested Ad5-TPC.

将带有LMP2A cDNA的重组质粒pGEM-T-LMP2A用EcoR Ⅰ、Sma Ⅰ双酶切下LMP2A cDNA，并将其插入含同样酶切位点的真核表达质粒pCIcc中，使其受控于CMV启动子下；用ClaI切下CMV-LMP2A-SV40表达单元，插入E1、E3区替代的腺病毒载体pAX1CW，选择正确的克隆pAX1CW-LMP2A与Ad5 DNA-末端肽复合体共转染293细胞，通过同源重组获得复制缺陷型的重组腺病毒(Ad5-LMP2A)。

Methods The immunofluorescency, Western blotting and RT-PCR were performed on mouse liver tissue obtained from 4 experimens pcDNA3.1(+-HBX and 1 normal control, 24 h after delivery the HBX gene eukaryon expression vector pcDNA3.1-HBX into mouse by the means of hydrodynamic injection.

实验动物分成模型组和对照组，模型组利用已构建的含有HBX基因的真核表达质粒pcDNA3.1-HBX，对照组以等量生理盐水代替，采用流体动力学法将质粒经尾静脉高压注入小鼠体内，24h后取小鼠肝组织，行免疫荧光、RT-PCR和Western blot法从不同水平检测HBX在小鼠肝组织内的表达情况。

To further elucidate the mechanism of BMP in regulating cell differentiation and to provide basis for BMP gene therapy, this study centers on the effects of BMP2 gene transfection on fibroblasts and contains the following works:① A 1.2kb fragment containing full length human BMP2 coding region was inserted into pBK-CMV (expressed both in protocaryon and eukaryon) by using predetermined orientation methods, resulting in phagemid expression vector for BMP2 (pBK-B2).② pBK-B2 was transfected into NIH3T3 cells by using LipofectAMINE.

为了进一步从分子水平探讨BMP对细胞分化的调控作用，并为BMP基因疗法的建立提供依据，本实验围绕BMP2基因转染对成纤维细胞的生物学作用进行了以下几个方面的工作：①将含有人BMP2全长编码序列的基因片段(1.2kb)定向克隆于噬菌粒表达载体pBK-CMV中，成功构建了人BMP2噬菌粒表达载体pBK-B2。

Under the company sets up the light quality calcium carbonate factory, the active calcium carbonate factory, the Heavy tar calcium carbonate factory, a calcium carbonate mother grain of factory, the special-purpose nanometer calcium carbonate factory and raw material factory six branch offices; Has the light quality calcium carbonate 50,000 ton, the active calcium carbonate 20,000 ton, the Heavy tar calcium carbonate 10,000 ton, the calcium carbonate mother grain 20,000 ton, the special-purpose nanometer calcium carbonate series product 10,000 tons with the calcium carbonate undressed ore 200,000 ton year productivity.

公司下设轻质碳酸钙厂、活性碳酸钙厂、重质碳酸钙厂、碳酸钙母粒厂、专用纳米碳酸钙厂和原料厂六个分支机构；具有轻质碳酸钙5万吨、活性碳酸钙2万吨、重质碳酸钙1万吨、碳酸钙母粒2万吨、专用纳米碳酸钙系列产品1万吨和碳酸钙原矿20万吨的年生产能力。

Verily, verily, I say unto you, Except a corn of wheat fall into the ground and die, it abideth alone: but if it die, it bringeth forth much fruit.

约12：24 我实实在在的告诉你们、一粒麦子不落在地里死了、仍旧是一粒。若是死了、就结出许多子粒来。

Verily, verily, I say unto you, Except a corn of wheat fall into the ground and die, it abideth alone: but if it die, it bringeth forth much fruit.

约12：24 我实实在在的告诉你们，一粒麦子不落在地里死了，仍旧是一粒。若死了，就结出许多子粒来。

Verily, verily, I say unto you, Except a corn of wheat fall into the ground and die, it abideth alone: but if it die, it bringeth forth much fruit.

024 我实实在在的告诉你们，一粒麦子不落在地里死了，仍旧是一粒，若是死了，就结出许多子粒来。

24 Verily, verily, I say unto you, Except a corn of wheat fall into the ground and die, it abideth alone: but if it die, it bringeth forth much fruit.

12：24 我实实在在地告诉你们，一粒麦子不落在地里死了，仍旧是一粒。若是死了，就结出许多子粒来。

Verily, verily, I say unto you, Except a corn of wheat fall into the ground and die, it abideth alone: but if it die, it bringeth forth much fruit.

12：24 我实实在在的告诉你们，一粒麦子不落在地里死了，仍旧是一粒。若死了，就结出许多子粒来。

Verily, verily, I say unto you," He said,"Except a corn of wheat fall into the ground and die, it abideth alone: but if it die, it bringeth forth much fruit.

他说︰&我实实在在地告诉你们，一粒麦子不落在地里死了，仍旧是一粒；若是死了，就结出许多子粒来。&

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。