粒

Dna efficiency and quality can be analysed by agarose gel electrophoresis and double enzymatic digestions.

结果 c、d法提纯的质粒dna的含量、纯度和收获率均较a、b高，上述4种方法提纯的质粒dna都能进行酶切反应。

PUC19 plasmid were successfully enriched and purified from the E. coli DH5α under the magnetic fields. The obtained plasmid retained native the bioactivity to be used for restriction enzyme digestions and cell transformations.

用这种方法成功地从大肠杆菌DH5α浓缩和纯化得到了pUC19质粒，该质粒具有生物活性，可直接用于限制性酶切和细胞转化等分子生物学下游操作。

The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

The results showed that the sizes of dopants were reduced, and the size distributions were narrowed.

结果发现，掺杂铅、La_2O_3后，二氧化钛的粒径明显降低，而且粒径分布也比较窄。

Deposited high quality armorphous ZnO and nanocrystalline ZnO films by electrophoretic deposition, strong ultraviolet emission peak was observed at room temperature, can be expected to have wide application in materials research. 3、Prepared Mn dopped and modified nanocrystalline ZnO.

将实验结果与理论计算进行了比较，结果表明，当粒径较大时，计算结果与实验结果吻合得较好，当粒径较小时，与实验结果有一定的偏差，并对其原因进行了分析。

The grain size of the material prepared by dopping Sr in the position "A" or dopping Co or Ni in the position "B" of the La_4BaCu_5O_13＋δ is small but it becomes bigger by dopping Ce in the position "A" of the La_4BaCu_5O_13＋δ.

SEM测试表明材料的粒径随着温度的升高而迅速增大，但颗粒的分布比较均匀。A位掺Sr和B位掺Co和Ni时合成的材料的粒径较细小，而A位掺Ce时合成材料的颗粒较大，这与BET测试和孔径分布测试相一致。

And the plant average of several endosperms of each plant as data set for QTL mapping.

方法Ⅰ是以分离群体中各植株的分子标记基因型以及植株上若干粒种子胚乳性状的单粒观察值为数据模式进行QTL分析

Based on Bayesian statistics and quantitative genetic model of triploid endosperm traits, we proposed a Bayesian method for mapping QTL underlying endosperm traits, which used the DNA molecular marker genotypes of each plant in segregation population and the single endosperm observation of a few endosperms of each plant as data set to analyze endosperm QTL.

将贝叶斯统计原理和胚乳性状的数量遗传模型相结合，以分离群体中各植株的分子标记基因型以及植株上若干粒种子胚乳性状的单粒观测值为数据模式，提出胚乳性状QTL区间作图的贝叶斯方法。

The density of the foot 、middle part and top are 124grain /m~2、91 grain /m~2、66 grain /m~2,the seeds is more little windward than the leeward.The density of seed banks in windward is 81grain/m~2 and the leeward density is 100grain/m~2.The seed of ephemerals bourgeoning reaches the pinnacle on April and May.The diversity of different parts sand dune is different,but the differences do not reach the marked level.The similarity is high between the seed banks and the vegetation.

迎风坡种子库密度比背风坡面少，迎风坡种子库平均密度为81粒/m2，而背风坡种子库平均密度为100粒/m2；短命植物种子在4月上旬达到萌发高峰，沙丘不同部位物种多样性存在差异，土壤种子库和地上植被存在较高的相似性，此外短命植物在沙丘不同部位的分布存在较高的空间异质性。

As for epidotesfrom blueschist, X_=0.22--0.36, and their composition varies remarkablyeven.in the same sample. In general, fine--grained epidotes contain relativelylow ps while things are just the opposite for coarse--grained ones whichcommonly exhibit zonal structure characterized by the gradual decrease of pstowards the core, suggesting that the bluescbist might have experienced apressuree--decline metamorphic process.

s=0.22—0.36，其成分在同一样品中也有很大变化，一般细粒者ps分子少，而粗粒者ps分子多，且常发育环带结构，向中心ps分子减少，说明蓝片岩可能经历了一个以降压为特征的变质过程。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。