粒

To test this hypothesis, we performed experiments using plasmids containing a luciferase reporter with amber, ochre and opal nonsense mutations within the luxB gene in Escherichia coli.

为验证此假设，我们用几个含有大肠杆菌荧光素酶报告基因的质粒进行了实验，这些质粒中的luxB基因分别包含有琥珀色、赭色和乳白色的无义突变。

I started out taking one pill twice per day when my father caught a bad cold. A few days later as his cold got worse, I started to get a dry stuffy nose, and I ramped up my dose of andrographis to 3 and 4 pills twice daily, and it seems to have eliminated the symptoms and prevented the cold from progressing further.

当我父亲得了重感冒时，我每天服用两次，一次一粒，几天后，他的感冒加重了，我也出现鼻塞，我加大穿心莲的剂量，一天两次，一次3-4粒，看起来止住了这些症状，防止的感冒进一步的加重。

Mixed lettuce topped with bleu cheese, chopped apple, walnuts, red onion, dicedcelery, and crisp bacon. served with dijon vinaigrette.

在新鲜的生菜上加上进口的蓝脉芝士、新鲜的苹果粒、核桃粒、洋葱、西芹丁及脆培根，另配法式

Referring to the research result of large stone asphalt mixture in foreign countries, thestructure and performance of large stone asphalt treated base have been researched.

本文参照国外对大粒径沥青混合料的研究成果，对大粒径沥青稳定碎石基层结构和性能进行研究。

If the resistance rises to the pre-set number(1245－1470Pa), the controller will send out signals, first to control the lift valve to do these operations: close the hole of the valve board to cut air current and stop filtering, and then to open the electromagnetic pulse valve to blow compressed air whose pressure is 0.5-0.7Mpa into filter bag with a short time(0.01－0.12s).

当含尘烟气由进风口进入灰斗以后，一部分较粗尘料在这时由于惯性碰撞、自然沉降等原因落入灰斗，大部分尘粒随气流上升进入袋室，经滤袋过滤后，尘粒被阻留在滤袋外侧，净化的烟气由滤袋内部进入箱体，再由阀板孔、出风口排入大气，达到收尘的目的，随着过滤过程的不断进行，滤袋外侧的积尘也逐渐增多，从而使除尘器的运行阻力也逐渐增高，当阻力增到预先设定值（1245－1470Pa）时，清灰控制器发生信号，首先控制提升阀将阀板孔关闭，以切断过滤烟气流，停止过滤过程，然后电磁脉冲阀打开，以极短时间（0.01－0.12s）向箱体内喷入压力为0.5－0.7Mpa的压缩空气，压缩空气在箱体内迅速膨胀，涌入滤袋内部，使滤袋产生变形、震动，加上逆气流的作用，滤袋外部的粉尘便清除下来掉到灰斗，清灰完毕之后，提升阀再次打开，除尘器又进入过滤状态。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The fragment was then cloned into the unique Xba Ⅰ site of the transfer vector pVL1393 by blunt-ended ligation.

目的基因片段和表达质粒载体pVL1393片段进行钝端连接，获得表达质粒载体pVL1393-HμOR-6His。

The tidal wetland sediment appeared the tendency of changing small from sea to land and from bottom to top.

结果表明，潮间带沉积物呈自海向陆和自下向上变细的趋势，一般光滩沉积物粒径〉50μm，盐沼上部沉积物粒径〈25μm；沿岸方向上，茂密盐沼的沉积物较之稀疏盐沼细；盐沼中的流速和波高较之相邻光滩减少50%以上；植物粘附悬沙干重达

Lo models with a horizontal mixer, biaxial pulp of the mixer, Central touch granulators, [250, 320, 350], water droplets grinder, mixer drum additives,(SR-enrichment Material equipment), bucket elevator, U-cutter-type carrier, there are die-pellet feed and other 30 kinds of ancillary equipment.

机型有卧式螺带混合机、双轴浆叶混合机、环摸制粒机、[有250型、320型、350型]、水滴粉碎机、鼓式添加剂混合机、、斗式提升机、U型输送绞龙、还有平模制粒机等30多种饲料配套设备。

The new factory possessed two sets of equipment of a concentrate and formula feed unit and a premix feed unit respectively; while, composing by one expanded feed production line, one bulk feed line and two pelleting lines, the concentrate and formula feed unit applies advanced high efficient grinding, compounding and pelleting equipment with the annual production capacity of 180,000 tons as well as conforming to the request of environmental protection and security.

由于鹤来公司的迅速发展，禾丰集团于2007年5月在北京市大兴区榆垡规划区内投资6500万人民币兴建现代化厂，并于2008年4月15日正式开业投产，新工厂配备两套设备分别为浓缩全价料机组和预混料机组，浓缩料机组拥有一条膨化料生产线、一条散装料线、两条制粒线，采用了国内先进的高效粉碎、混合以及制粒设备，并符合环保和安全要求，年生产能力为18万吨。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。