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Adult rats are divided into control group, intrapericardial injection group and negative group, sublingual vein injection group and negative group. 6ds after transfection, issues of heart, liver, lung and kidney are stained with X-gal to observe transfection to myocardium and non-target transfection. 3. CaN Aβ gene silencing in vivo. Adult rats are divided into Control group, hypertrophy model group, intrapericardial injection group and negative group, sublingual vein injection group and negative group.

二整体动物水平上探讨心肌有效转染途径:以PLacZ为报告基因,将成年大鼠分为空白对照纽(生理盐水心包腔内注射后超声导入);转染质心包腔内注射组及其阴性对照组:质+微泡+酶的混合物心包腔内注射,超声导入;转染质舌下静脉注射组及其阴性对照组:质+微泡混合液经舌下静脉注射,超声导入。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米子、修饰有端酶反义寡核苷酸的磁性纳米子诱导HL-60细胞发生凋亡,原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化:细胞核固缩,核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘,并形成凋亡小体。2 磁性纳米子、修饰有端酶反义寡聚脱氧核苷酸的磁性纳米子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用,与对照组相比有显著性差异(p<0.01),在剂量为0.8-8μmol/L范围内,抑制率随剂量的增加而增加,当剂量超过8μmol/L时,抑制率反而下降;3 磁性纳米子、修饰有端酶反义寡聚脱氧核苷酸的磁性纳米子可增强p53基因的表达活性,引起DNA降解损伤,反向调节细胞周期活动,促使细胞从G0期进入G1期,抑制肿瘤细胞的生长。4 修饰有端酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核,可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

In the present paper, a theoretical model of the mobility for multidisperse particles is developed. The present model is the extension of the previous theoretical model and the semi-empirical model for the monodisperse partcles. This model includes the influence of particle size distributions and is suitable for all particles with different materials and with different size distributions.

通过理论分析,本文提出了多分散性尘电迁移率数学模型;此模型是典型的电迁移率模型和单分散性尘电迁移率半经验模型的扩展,它考虑了度分布的影响,适用于各种物质的尘和各种度分布的尘

Electron microscope observations showed that the liposome suspension was heterogeneous and the particle size ranged from 20 nm to 700 nm with a average diameter of 163 nm. The vesicles showed unilamellar and multilamellar structure which consisted of several concentric bilayers.

电镜观察表明,该脂质体多呈球形的单层或多层结构,脂质体径分布均匀,径范围20-700 nm,平均径163 nm,主要径分布位于50-200 nm(84.80%)。

Formation of mating pairs, nicking of the F oriT sequence, and transfer of the 5' end of a single strand of F DNA proceed as in transfer of the F plasmid. Therefore, Part of the plasmid is transferred first, Chromosomal genes are transferred next, and the rest of the plasmid is transferred last.

雌雄菌的交配、F质oriT处缺口的形成、以及由单链F DNA5'末端开始转移的过程都与F质的转移相同,因此,首先转移的是部分质片段,其次是染色体基因,剩余的质片段最后被转移。

One could nodulate the soybean cultivars tested and showed wider host range, the other only nodulate limited soybean cultivars. Plasmid could not be detected from twenty-three slow-growing strains and twenty-nine fast-growing strains were found containing 1-4 plasmids by Echardts method.

快速检测结果表明:慢生型大豆根瘤菌均不含有质,快生型大豆根瘤菌均有1-4个质,质类型与宿主亲和性也有一定的相关性。

Or white blood cell; a colorless blood corpuscle capable of ameboid movement, whose chief function is to protect the body against microorganisms causing disease and which may be classified in two main groups: granular (granulocytes: basophils, eosinophils, neutrophils) and nongranular(nongranulocytes: lymphocytes, monocytes).

或称白血球,为一能作变形运动的无色血细胞,其主要作用是保护机体抗御致病菌。它可以分为两大类:性(性白细胞:嗜碱细胞、嗜酸细胞、嗜中性细胞)和非性(非性白细胞:淋巴细胞、单核细胞)。

According to the year with the best large-seed production, the reasons for empty seeds could be estimated as follows: Seed potential per cone calculated as 2 times the number of fertile ovuliferous scales was about 206 seeds. Percentages of seed losses resulting from pre-and post-pollination ovule abortion, prezygotic factors, embryo degeneration, and efficient seed were 33%, 27%, 39%, and%, respectively.

依据大种子产率最高的1年来估测空种子产生的因素,可以推估如下:依据每个可孕性鳞片所含2种子的鳞片数,潜力种子数约有206,再依此值算出受粉前后胚珠败育率、受精前败育率、胚败育率、及有效种子率分别为33%、27%、39%及%。

Because of nitrogen, the value of IAA/ABA、GA3/ABA、(IAA+GA3+ZR)/ABA in grain promoted at seed filling stage and at the mature stage.6、The adjustment of nitrogen levels on carbon and nitrogen metabolism.Nitrogen reduced the C/N in leaves and stem. When the nitrogen rate was 50 kg/hm2, the effect on stem was obvious. When the nitrogen rate was 100 kg/hm2, the effect on leaves was obvious. The C/N in roots lower before seed filling stage and the effect was not obvious after seed filling stage.7、Effect of nitrogen levels on yield traitsPlant height, pods per plant and full grain number were increased and bottom-pod height and podded node were decreased by applying nitrogen.

施氮在鼓期、成熟期均提高了子中IAA/ABA、GA3/ABA、(IAA+GA3+ZR)/ABA的值。6、氮素水平对大豆碳氮代谢的调节施氮降低了叶片和茎秆中C/N的比值,施氮量为50 kg/hm2时,对茎秆作用效果明显,施氮量为100 kg/hm2时,对叶片效果明显;施氮降低了根系鼓期前的C/N比值,而鼓期后作用效果不明显。7、氮素水平对产量性状的影响施氮增加了大豆的株高、株荚数、饱满数,降低大豆的结荚高度、结荚结位。

Effect of protein and lipid in rice on starch physicochemical characteristics, difference between the region of non-chalkiness and chalkiness of rice, dynamic variation of cell structure and CaM distribution on rice spike were expounded in this study. It was found that protein and lipid in rice had important effect on starch gelatinization, there were distinct differences between the region of non-chalkiness and chalkiness of rice, vascular bundle system in rice spike was changed during grain filling, difference quality of different position of rice spike was more distinct in dense spike rice for more powerful vascular bundle system, the development of rice seeds was corresponded to pericarp development, difference quality of different position of rice spike was also related to transport function of rachilla besides activity of "source", and calmodulin relative activity was closely related to grain filling velocity and enginery properties.

通过实验发现:稻米中蛋白质、脂肪对淀粉的糊化过程有着较大的影响;精米的非垩白与垩白部位以及心白与腹白部位之间在蛋白质、氨基酸含量及组成方面存在着明显差异:水稻穗部大维管束面积以及主穗轴维管束数目在灌浆过程中存在明显的动态变化趋势;密穗型水稻强势的维管组织比散穗型水稻发达,致使其强、弱势维管组织差异明显加大;果皮的发育与籽发育状况是相适应的:强势和弱势在灌浆生理方面的差异,不仅表现在籽本身的&库活性&上,而且也与其着生小穗轴的输导功能有关;CaM相对活性与水稻灌浆速率、机能特征间存在着密切联系。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。