粒

In this study, the structure sequences of Shiga toxin 2B(stx2B) and Shiga toxin 1B(stx1B) were respectively amplified from EHEC O157:H7 by PCR. stx2B-L-stx1B fusion gene was constructed by a 12 bp linker ligating these two fragments. After digested with restriction endonuclease Nco Ⅰ and EcoR Ⅰ, the fusion gene was orientally inserted into expression vector pET28a.

利用PCR方法，从肠出血性大肠杆菌O157：H7的基因组DNA中，分别扩增出Stx1B和Stx2B亚基的结构基因片段，然后再通过PCR将这2个片段连接起来，并定向克隆到表达质粒pET28a的NcoⅠ和EcoRⅠ位点之间，构建了重组表达质粒pMB1。

The increased amount of non--exchangeable NH4^＋-N during the incubation indicated as the following tendency, namely, Eum-Orthic Anthrosols 〉 Los - Orthic Entisols 〉 Hap-Ustic Isohumisols 〉 Ust-Sandiic Entisols. Organic material influenced significantly the increased amount of non-exchangeable NH4^＋-N （P was 0.0002, 0.0004 and 0.0003 when incubated at 20 d, 50 d and 60 d, respectively）. NH4^＋-N increased remarkably when Stlpa bungeana and Medicago sativa were added compared with no addition. The contents of non-exchangeable NH4^＋-N increased significantly when added （NH4）2SO4 compared without addition at 20 d, 40 d and 60d of incubation and P was 0.0037, 0.0033 and 0.0027, respectively. It was the result that the NH4^＋-N from （NH4）2 SO4 was fixed within the soil. The increased amount of non-exchangeable NH4^＋-N increased significantly for different soils, on which different vegetation types grew before the collection of soil samples, when incubated at 20 d （P=0.0434）, but not significantly at 40 d and 60d （P=0.7378 and 0.5375）. The increased amount of non-exchangeable NH4^＋-N in the soil, on which crop straw and nitrogen fertilizer had been incorporated for a long-term period, was larger than that of no addition, but it was not significantly different among these two fertilization models. Soil clay, total N and organic matter were positively correlated remarkably with the contents of non-exchangeable NH4^＋-N, the increased amount of non-exchangeable NH4^＋-N had no correlation with soil clay, whereas it had significantly positive correlation with total N and organic matter.

培养期间非交换性NH4^＋-N的增加量均表现为以土垫旱耕人为土最大，其次是黄土正常新成土，简育干润均腐土和干湿砂质新成土较小；添加有机物料极显著影响培养期间的非交换性NH4^＋-N增加量（培养20d、40d和60d时P分别为0.0002，0.004和0.0003），表现为紫花苜蓿和长芒草土壤非交换性NH4^＋-N的增加量均极显著高于不添加有机物料的对照土壤；在培养20d、40d和60d时，加（NH4）2SO4土样非交换性NH4^＋-N的增加量显著大于不加（NH4）2SO4土样（户分别为0.0037，0.0033和0.0027），这是土壤对（NH4）2SO4中NH4^＋-N固定的必然结果；不同植被类型土壤培养20d时的非交换性NH4^＋-N增加量差异显著（P=0.0434），培养40d和60d时差异不显著（p分别为0.7378和0.5375）；长期秸秆和氮肥配施土壤非交换性NH4^＋-N增加量大于不施肥对照土壤，但差异不显著土壤黏粒、全氮和有机质与培养0d、20d、40d和60d时土壤的非交换性NH4^＋-N含量均呈极显著正相关；而非交换性NH4^＋-N的增加量与粘粒无相关性，但与全氛和有机质呈显著正相关。

The sample particles studied in this project include human red blood cells, osmotically swollen into a micro-sphere, with and without treating with N-ethylmaleimide, and at different temperatures, as well as liposomes with different DOPC/cholesterol ratio (1:1 vs.1:3) For spherical RBCs, smaller deformation were observed in samples at room temperature with NEM treatment and samples at 42 ℃without NEM treatment, compared with the deformation of the control set, namely, RBCs at room temperature without NEM treatment.

这可能是由於42 ℃为红血球细胞骨架Spectrin的型态转换温度，Spectrin由tetramer转换成dimer。推测此温度及药物作用下对Spectrin的型态转换可能造成类似的效果。同时以自行合成DOPC/胆固醇比例(1：1)及(3：1)之微脂粒重覆上述之形变实验，发现两种组合比例微脂粒形变率的差异在误差范围内。

The mechanical properties of coal filled polymer composites The mechanical properties of coal/PP composites were investigated, and the effects of coal rank, particle size, surface modification of coupling and alkylation were discussed. The results show that the anthracite and bituminous coal all can be used as filler of plastic after pretreatment of part devolatilization and surface modification; The modulus of composites increase with the content of coal filler; The coupling agent is of no effect for reinforcing the coal/PP composites, except that there are new oxygenous function group forming on the surface in the process of pulverizing; The F-C alkylation reinforce coal (under 10μm)/PP composites obviously.

4煤填充高分子复合材料力学性能超细煤粉填充聚丙烯复合材料力学性能的研究结果表明，烟煤和无烟煤经过脱除部分挥发分的预处理后，都可用于与塑料共混制备复合材料；偶联剂改性对低变质程度烟煤/聚丙烯复合材料的力学性能基本不起改善作用，而对于经气流超细粉碎后的小粒径高变质程度烟煤及无烟煤则有一定的增强作用；烷基化改性在煤表面接枝了烷基，使平均粒径小于10μm的煤/聚丙烯复合材料拉伸屈服强度普遍提高，煤填充高分子复合材料可在较高含煤量情况下维持良好的力学性能。

Plasmid DNA of pBILGC containing Chitinase and β-1, 3 - glucanase gene and fresh pollen of Papaver nudicaule were mixed in 11％ sucrose solution, then the pollen suspension were pollinated on stigmas of the Papaver nudicaule by artificial pollination.

以野罂粟开放花蕾为受体，以载有几丁质酶和β-1，3-葡聚糖酶基因双价植物表达载体pBLGC的质粒为供体，在11％浓度的蔗糖溶液中，加入花粉和含有目的基因的质粒DNA。得到药粉处理液。

The analysis shows that under heat stress, meiocytes degenerate while tetrad cell dispersion fails at microspore formation phase. During pollen maturation phase, loss of orientation of reproductive cells, abnormal vacuolization of tapetal cells, paramorphia or abortion of anther walls and pollens are observed under heat stress.

结果表明，受高温胁迫后，在小孢子形成阶段，水稻花粉母细胞发育异常、四分体无法适时分散；在花粉粒成熟阶段，水稻生殖细胞失去正确的排列方向、绒毡层细胞发生非正常分解、花药壁和花粉粒发生形态异常并出现败育。

The effects of the spray-drying process of the ammonium paratungstate and the carburization temperature on the formation of the precusor and WC powder were investigated.

采用喷雾干燥-固定床法制备碳化钨粉末，研究了喷雾干燥和碳化反应温度对制备偏钨酸铵前驱体以及碳化钨的影响，前驱体粉末呈现均匀的球状形态，平均粒径为10~20μm，喷雾温度对影响粒径大小和分布影响小；400℃的进口温度下进行喷雾干燥，喷雾粉末仍然保持偏钨酸铵原来的基本组成不发生分解。

In Salsola passerina -Reaumuria soongorica community, the first ordination axes explains the salinization gradient, along the order of Caragana tibetica community,Salsola passerina-Oxytropis aciphylla community,and Reaumuria soongorica-Salsola passerine community,soil alkalization increases. The second ordination axis explains soil structure gradient, along the order of Caragana tibetica community, Reaumuria soongorica-Salsola passerina community and Salsola passerina community, soil texture becomes coarser. In the Stipa breviflora-Stipa grandis community, the first ordination axis indicates the soil water gradient, and the second ordination axes explains hydrothermal coupling gradient. In the Prunus mongolica-Ulmus glaucescens community, the first ordination axis explains the soil pH gradient, along the order of Ulmus glaucescens-Prunus mongolica community, Prunus mongolica-Potentilla fruticosa community, and Potentilla fruticosa-Prunus mongolica community, soil pH value reduces. The second ordination axis explains soil structure gradient, along the order Prunus mongolica-Ulmus glaucescens community, and Prunus mongolica-Potentilla fruticosa community, the contents of silt and clay increase, and soil texture suggests a fine trend.

在珍珠猪毛菜-红砂群落，第一排序轴反映了土壤盐碱化梯度，沿着藏锦鸡儿群落—珍珠猪毛菜、猫头刺群落—珍珠猪毛菜、红砂群落序列，土壤盐碱化程度不断增强；第二排序轴则反映了土壤结构梯度，沿着藏锦鸡儿群落—珍珠猪毛菜、红砂群落—珍珠猪毛菜、猫头刺群落序列，土壤质地逐渐粗化；在短花针茅-大针茅群落，第一排序轴反映了土壤水分梯度，第二排序轴反映了海拔梯度上的水热组合梯度；在蒙古扁桃-灰榆群落，第一排序轴反映了土壤pH梯度，沿着灰榆、蒙古扁桃群落—蒙古扁桃、金露梅群落—蒙古扁桃群落序列，土壤pH值逐渐下降；第二排序轴主要反映了土壤结构梯度，沿着蒙古扁桃群落—灰榆、蒙古扁桃群落—蒙古扁桃、金露梅群落序列，土壤中粉粒、粘粒含量逐渐增加，土壤质地呈细化趋势。

In Salsola passerina-Reaumuria soongoriea community, the first ordination axes explains the salinization gradient. along the order of Caragana tibetica community, Salsola passerina-Oxytropis aciphylla community, and Reaumuria soongorica-Salsola passerine community, soil alkalization increases. The second ordination axis explains soil structure gradient, along the order of Caragana tibetica community, Reaumuria soongorica-Salsola passerina community and Salsola passerina community, soil texture becomes coarser. In the Stipa breviflora-Stipa grandis community, the first ordination axis indicates the soil water gradient, and the second ordination axes explains hydrothermal coupling gradient. In the Prunus mongolica-Ulmus glaucescens community, the first ordination axis explains the soil pH gradient, along the order of Ulmus glaucescens-Pnuius mongolica community, Prunus mongolica-Potentilla fruticosa community, and Potentilla fnuicosa-Prunus mongolica community, soil pH value reduces. The second ordination axis explains soil structure gradient, along the order Prunus mongolica-Ulmus glaucescens community, and Prunus mongolica-Potentilla fruticosa community, the contents of silt and clay increase, and soil texture suggests a fine trend.

在珍珠猪毛菜－红砂群落，第一排序轴反映了土壤盐碱化梯度，沿着藏锦鸡儿群落－珍珠猪毛菜、猫头刺群落－珍珠猪毛菜、红砂群落序列，土壤盐碱化程度不断增强；第二排序轴则反映了土壤结构梯度，沿着藏锦鸡儿群落－珍珠猪毛菜、红砂群落－珍珠猪毛菜、猫头刺群落序列，土壤质地逐渐粗化；在短花针茅－大针茅群落，第一排序轴反映了土壤水分梯度，第二排序轴反映了海拔梯度上的水热组合梯度；在蒙古扁桃－灰榆群落，第一排序轴反映了土壤pH梯度，沿着灰榆、蒙古扁桃群落－蒙古扁桃、金露梅群落－蒙古扁桃群落序列，土壤pH值逐渐下降；第二排序轴主要反映了土壤结构梯度，沿着蒙古扁桃群落－灰榆、蒙古扁桃群落－蒙古扁桃、金露梅群落序列，土壤中粉粒、粘粒含量逐渐增加，土壤质地呈细化趋势。

Total # of buttons=6, Have your patternmaker respace again in the placket.

总共是6粒钮，让你们的纸样师傅重新定钮位因减了两粒钮，所以重新定位是必要的

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。