粒

Synergistic down|regulation of telomerase activity and hTERT mRNA expression by combination of retinoic acid and GM|CSF in human myeloblastic leukemia ML|1 cells.

端粒酶激活过程中受到严格调控的部分是其蛋白亚单位，是否呈现端粒酶活性，主要是由蛋白亚单位的表达与否来决定的[6，7]。

ABSTRACT］ObjectiveTo study the expression of telomerase activity in patients with acute myelocytic leukemia and its clinical significance.

摘要］目的研究急性髓细胞白血病的端粒酶活性表达情况，探讨端粒酶活性检测对AML的临床意义。

By means of the electron microscopic enzymo cytochemistry, it was observed that the activity of acid phosphatase was localized on the autophagic vacuole, the enzyme reaction granules gather to form the vacuolar bodies of various shapes in the cytoplasmic region of the osmiophilic and paraglycogen granules in resting Stylonychia mytilus .

该文应用电镜酶细胞化学方法显示，贻贝棘尾虫休眠包囊中，在胞质嗜锇粒和拟糖原粒区，酸性磷酸酶反应颗粒聚集于具有消化泡作用的泡状体上，葡萄糖 6 磷酸酶反应也定位在自噬泡膜位置。

To construct a plasmid for analyzing gene functions and expressions and to study the MXAN1334 gene in Myxococcus xanthus with the plasmid.

旨在构建一个用于基因插入失活，并同时可通过报告基因分析插入位点基因表达情况的质粒载体，并应用该质粒对黄色粘球菌MXAN1334基因功能和表达情况进行分析。

It was observed that although the pSym-curred strain HND29SR could not nodulate on the host plant Heinong 33, there was no difference in the root colonization dynamics and density of rhizobium population between HND29SR and its wild-type HN01 or reference strain HN01L in sterilized pot-sand system.

这一现象说明HN01所含共生大质粒不影响其根圈定殖行为，有理由推测影响HN01根圈定殖行为的有关基因可能存在于染色体上或另外两个内源质粒上。

The stability of GA-PBCA-NP was also studied.ResultsDue to the agglomerationg of nanoparticles with no stabilizer,it was difficult to preparate.GA-PBCA-NP produced by the two methods both had spheric and smooth outer appearance,uniform distribution,nonadherence and stability.The diameter,ebeding ratio and drug loading in GA-PBCA-NP produced by emulsification polymerization and interfacial polymerization methods were 90～600 nm and 200 nm,60.49% and 73.87%,18.15% and 22.23%,respectively.

结果未添加稳定剂的纳米球表面粘连团聚现象严重，难以成球；乳化聚合法制备的GA-PBCA-NP在透射电镜下观察表面圆整光滑，粒子之间不团聚，不黏连，粒径范围在90～600 nm，包封率为60.49%，载药量为18.15%；界面缩聚法制得的纳米粒表面圆整光滑，粒子之间不团聚，不黏连，粒径大小均匀，都在200 nm左右，包封率为73.87%，载药量为22.23%，两种方法制备的稳定性都较好。

GA-PBCA-NP produced by the two methods both had spheric and smooth outer appearance,uniform distribution,nonadherence and stability.The diameter,ebeding ratio and drug loading in GA-PBCA-NP produced by emulsification polymerization and interfacial polymerization methods were 90～600 nm and 200 nm,60.49% and 73.87%,18.15% and 22.23%,respectively.ConclusionIt is important to add dextran 70 in preparation.

结果未添加稳定剂的纳米球表面粘连团聚现象严重，难以成球；乳化聚合法制备的GA-PBCA-NP在透射电镜下观察表面圆整光滑，粒子之间不团聚，不黏连，粒径范围在90～600 nm，包封率为60.49%，载药量为18.15%；界面缩聚法制得的纳米粒表面圆整光滑，粒子之间不团聚，不黏连，粒径大小均匀，都在200 nm左右，包封率为73.87%，载药量为22.23%，两种方法制备的稳定性都较好。

The resulting strain, designated YES2MTM1, was transformed with a yeast genomic library. Transformants lost the plasmid overexpressing MTM1 after 5-FOA treatment. Yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and overexpression of some DNA fragments were picked up and candidate suppressor genes were identified. Overexpression of five genes were identified to be able to rescue the growth defect on nonfermentable carbon source. The study will provide reference for MTM1 gene function and screening for suppressor of genes whose deletion result in irreversible damage.

为了避免MTM1缺失造成的不可逆损伤，在野生型酵母中先转入带有MTM1 基因的质粒，再敲除染色体上的MTM1 基因，随后转入基因组文库，再利用药物5-氟乳清酸(5-FOA)迫使细胞丢失表达MTM1基因的外源质粒，再筛选能在非发酵培养基上生长的转化子，通过这种方法筛选发现，POR2等5个基因的过表达可以挽救MTM1 基因缺失造成的非发酵培养基上的生长缺陷，为深入了解MTM1基因的功能提供了线索，对筛选其他造成不可逆损伤的突变基因的抑制基因提供了一条可行的研究思路。

Nontronite modified copper-plated electrodes possess the largest reductive currents of paraquat due to their larger charge density and electronic transferability of octahedral iron. Overall, based on this study, clay modified copper-plated electrodes show high potential in future research for determining biochemical compounds.

黏粒修饰镀铜电极对巴拉刈的侦测结果显示三种黏土矿物仍以具有较高电荷密度及八面体层中铁的电子传导之多铁蒙特石所测到的还原电流量最大，黏粒修饰镀铜电极未来在生化物质的侦测上应具有研究发展的潜力。

After being digested with EcoR I and Xho I, they were inserted to the vectors pGEX-4T-l orientally, and then transformed into E.coli BL21(DE3) cells. The monoclone were selected and identified by means of restriction analysis, PCR and sequencing. As the result, the two recombinant plasmids with the different DNA fragements of the PrP gene were constructed and designated as pMY01[Ov rPrP(23-244)] and pMY02[Ov rPrP(91 -244)], respectively.

挑选单个菌落，提取重组质粒，经酶切、PCR扩增后琼脂糖凝胶电泳分析和测序鉴定，成功地构建了2种绵羊重组朊蛋白的表达质粒pMY01[OvrPrP(23～244)]和pMYO2[Ov rPrP(91～244)]。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。