粒

Image analysis based on coarse aggregate shape and morphology assessment has been of recent research efforts to successfully indices to results from manual testing methods as well 3 as to pavement response and performance. The objective of this study is to to observes the moves of coarse aggregate of stone mastic asphalt and dense asphalt concrete by using image analysis technique.To establish the model of coarse aggregate moving behavior and asphalt pavement rutting.

本研究计画由载重过程，以影像处理分析技术观察SMA 级配及密级配粗粒料移动轨迹变化，以定量描述SMA 级配粗粒料构架形成机制、抗车辙能力成因、粗粒料材料形状组合因子偏差等影响，建立粗粒料移动轨迹与车辙量之行为模式，深入了解SMA 材料载重行为，俾供应用於公路及机场铺面能完全发挥其特性。

The Shaoxing Tongyi needle spins limited company Two-sided velvet、 Swings the grain of fabric Double brushes Shan Yao Cuts 、 Coral fabric、 Side package、serging Border closing、overhand Stretches the seam、flatseam Above textile patterns and outside material are part of bills which produced by the company recently, if you have any quesqion, please touch me by telephone or E-mail .

主要产品有各种规格的单双面绒、羊羔绒、摇粒绒、剪毛绒、毛巾布、毛圈布、地裂布、珊瑚绒、超柔绒、印花摇粒绒、印花单双面绒、纸印花、抽条绒布和1-7#麻灰摇粒绒布及高F超细摇粒绒等各类高中档针织绒布。还可跟据客户要求，设计订做各类成品。以上是本公司最近走的单子中的一部分花型和面料，如有不清楚之处请来电或E-mail咨询。谢谢合作！

In the sorption equilibrium experiments, the results showed that the equilibrium sorption coefficients for VOCs sorption on clay and organoclays decreased with increasing relative humidity, which proved that the existence and amount of water indeed suppressed the sorption capacities for clay and organoclays to sorb VOCs.

在平衡实验部分，挥发性有机化合物在黏粒或有机黏粒的吸附平衡常数皆随著相对湿度的增加而减小，证实水分的存在及多寡确实会抑制黏粒及有机黏粒对於有机化合物的吸附能力。

The materials: Silver: 4500g, coral: 749 tablets, crystal: 139 tablets, pine stone: 398 tablets, pearl: 20 tablets.

此产品纯银作旧，由大约为：白银4500克，珊瑚749粒，青晶139粒，松石398粒，珍珠20粒制成。

The number of the chromosomes in Henan large tail sheep is 2n=54, the karyotypes of male and female sheep are 54, XY and 54, XX, respectively. There are 26 pairs of autosomes and 1 pair of sex-chromosome. Among 26 pairs of autosomes, 3 pairs are metacentric chromosomes, 23 pairs are telocentric chromosomes. X chromosome is the biggest subtelocentric chromosome and Y chromosome is the smallest submetacentric chromosome.

河南大尾寒羊二倍体染色体数目为2n=54，公羊核型54，XY；母羊核型54，XX.27对染色体中包括26对常染色体和1对性染色体，常染色体中有3对为中着丝粒染色体，23对为端着丝粒染色体；性染色体中，X染色体为最大的端着丝粒染色体，Y染色体为最小的中着丝粒染色体。

The onset temperature (T0), peak temperature, conclusion temperature and gelatinization enthalpy in DSC thermogram of rice starch were higher for the samples of high temperature regime than for the samples of optimum temperature.

不同品种类型间虽然存在着一定的差异，但变化规律基本一致；灌浆结实期温度明显影响淀粉粒的粒径大小，表现为高温处理下，直径大于7协m的大淀粉粒明显增多，而直径小于4林m的小淀粉粒明显减少。

HRP was coupled with the fluorochrome FITC and encapsulated with liposome . pEGFP-N1 plasmid (expressing green fluorescent protein) with encapsulation in commercially available liposome was prepared. The eyes were enucleated 1, 4 and 8 weeks after zonule rupture and anterior segments comprising lenses were incubated in medium containing one of these components. Cryo-sections were made and translocation of fluorescent macromolecule from the medium into the lens and green fluorescent protein expression in the epithelium were observed by fluorescent microscopy.

制备FITC标记的HRP脂质体；制备pEGFP-N1质粒（表达绿色荧光蛋白的质粒），并用脂质体包裹；豚鼠悬韧带部分离断后1周、4周、8周取含晶状体的眼前段标本分别在含有FITC-HRP复合物、pEGFP-N1质粒的培养基中孵育，冰冻切片，荧光显微镜观察FITC-HRP复合物进入晶状体和pEGFP-N1质粒在晶状体内表达的情况，比较悬韧带离断侧与非离断侧的差异。3。

After the NK gene from recombinant plasmid pUC19-NK was cloned on expression vector pBV220, it was transformed into host cell E. coli HB101. Recombinant plasmid pBV220-NK was extracted for identifying by analysis of restriction enzymes, PCR and sequencing. Studies on recombinant strain's growth and expression showed that heterogene have no distinct effect on host cell. The recombinant plasmid have excellent segregational stability but low structural stability. The results of kinetics of pBV220-NK expression in E.

将重组质粒pUC19-NK上的纳豆激酶基因成功克隆到表达载体pBV220上，并将之转入大肠杆菌HB101中，得到转纳豆激酶基因工程菌，提取重组质粒经单酶切、双酶切及PCR分析验证后，对重组菌的生长及表达进行一系列研究，结果表明：外源纳豆激酶基因的导入对宿主的生长没有明显的影响：重组质粒的稳定性实验表明：该质粒具有良好的分离稳定性，而结构稳定性较差。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Telomere, a complex structure present at the ends of eukaryotic chromosomes, consists of tandam arrays of highly conserved hexameric repeats in vetebrates. Its function is to protect DNA from degradation of deleterious recombination.

端粒是由许多简单重复序列及相关蛋白组成的染色体末端复合结构，端粒酶是具有逆转录酶特性的核糖核蛋白复合物，能以自身的RNA为模板合成端粒重复序列，加至新合成的DNA链末端，维持端粒平衡从而保证染色体末端结构完整性及解决末端复制问题。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。