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In this paper, an escherichia coli-saccharopolyspora erythraea shuttle vector containing the erya promoter region was constructed using the enhanced green fluorescent protein gene as a reporter. the shuttle plasmid was transformed into sac.erythraea a226 and streptomyces lividans jt46 by peg mediation, respectively. fluorescence microscopy confirmed that the egfp was expressed in both strains.

本文克隆了erya基因的启动子perya,以绿色荧光蛋白基因为报告基因,构建了大肠埃希菌-糖多孢红霉菌穿梭型质。peg介导原生质体转化法将穿梭型质分别转入糖多孢红霉菌a226与变铅青链霉菌jt46,荧光显微镜检测发现,此启动子在两菌株中都具有功能。

As most Streptomyces promoters are invalid in S. erythraea, the promoter of erythromycin-resistant gene, ermE, and Streptomyces chromosomic integration site, attP, and apramycin-resistant gene, apr from plasmid pSET152 are utilized to construct the expression vector pZMW.

因大多数链霉菌质的启动子在糖多孢红霉菌中都不能发挥作用,故利用糖多孢红霉菌染色体上红霉素抗性基因PermE启动子作表达载体启动子,并利用pSET152质上链霉菌染色体整合位点和氨朴霉素抗性基因,构建了表达载体pZMW。

PinX1 is an evolutionally conserved nucleolar protein that has been recently demonstrated as a telomerase/ telomere interacting factor in both the humans and the yeasts.

PinX1是近年在人和酵母细胞中发现的一个能与端/端酶相互作用的保守核仁蛋白。

Regardless to the research development or industrial application, nano metal powders are extensively discussed. Within these powders, the flammable and explosible nano aluminum, titanium and iron powders produce a great amount production and apply widely. Meanwhile, the surface/volume ratio of powders is increased significantly under nano scale, in which to enhance the ignition opportunity. Thus, the dust explosion hazard risk of nano powders could remarkable rise. In addition, the comparison of explosion phenomenon, test methods and quantification parameters between nano and micro scale is necessary to deeply investigate further. So that this project operates the explosion characteristic experiments to measure and determine such the maximum explosion pressure, minimum explosion concentration and explosion characteristic Kst value on above mentioned three nano metal powders. Expectantly, to provide the appropriate recommendations of safety prevention for nano dust explosion based on the experimental results.

奈米金属粉末无论是研究发展或是工业界应用,目前皆是广泛讨论研究之材料,其中,奈米铝粉、奈米钛粉以及奈米铁粉等可燃、爆性粉末,均有高额产量及大量应用领域,与此同时,在粉末径进入奈米尺度的情况下,粉尘比表面积大幅提升,增加此三种粉尘可被点燃的机会,因此,潜在的粉尘爆炸危害风险将广幅增加,另外,微米尺度粉尘爆炸的现象、测试方法以及量化参数是否具有相同的实验尺度基点,也需要深入探讨,故本次计画针对上述三种奈米金属粉末,进行三种不同径的爆炸特性参数实验,如最大爆炸压力、最低爆炸浓度以及爆炸特性参数Kst值之量测与计算,期能量化其粉尘爆炸特性,并据此提出适当的安全防护建议。

The glutamate oxaloacetate transaminase isozyme of thirty-three accessions of cultivated and wild buckwheat belonging to eight species of genus Fagopyrum including seven species of the big-achene group and one species of the small-achene group were studied by means of polyacrylamide gel eletrophoresis.

用聚丙烯酰胺凝胶电泳技术时荞麦属8个种(含大组7个种和小组1个种)33份荞麦资源的谷草转氨酶同工酶进行了研究。

The results showed that rice straw growth and fascicular alteration interacted when water was deficient. It not only affected the transportation of nutritious substance from rhizine and leaf to kernel, but also led to the decrease of agronomic characters and yield. 4 ecotypes of rice vascular bund...

结果表明:水分不足对稻秆及秆中维管束的影响是相互的:稻秆的发育情况影响了维管束变化,同时维管束的变化影响了稻秆的发育,最终影响营养物质从根部、叶部向籽运输,从而使结数、稔实率、千重、产量下降。4种生态型水稻维管束系统与一次及高次枝梗数目、结数、千重等有类似的相关性,而且水分不足时反应出相同的结果,说明土壤水分对维管束系统的分化有很大的影响,水分不足将使维管束面积变小,维管束比增大,从而使产量变小。

The fluidal peperite is produced by the mingling of magma with fine sediments, while the blocky peperite is related to coarse sediments.

流状熔积岩多是熔浆与细沉积物混合的结果,块状熔积岩大多与粗沉积物相关。

It is more or less taste like traditional Foochow cake.

这间店一天可卖出超过50,每Rm1。

The studies on the compositions, origins and elastic properties of the southern Tibetan lower crust include the following 4 aspects: the petrological and geochemical festures and ages of granulitic facie rocks located in Higher Himalayas; dehydration melting experiments on the biotite-plagioclase gniess collected from Higher Himalayas at high temperature and high pressure to model the formation of the granulites; measurements of wave velocities of the lower crustal rock in situ; analysis of origin of intracrust low wave velocity layers beneath southern Tibet.

以西藏南部下地壳物质组成,成因及其弹性性质为研究目标进行了4个方面的相关研究:高喜马拉雅地区麻岩相岩石的岩石学,地球化学和年代学研究;麻岩相岩石成因的高温高压实验;原位条件下岩石的弹性波速测量;西藏高原壳内低速层的成因分析与模拟。

Among them, the black rings puts in order salt crust sand content to increase, but the white rings puts in order by the silt and the clay primarily. The light and shade interaction annulus of salt crust granulometric composition difference are showed that the saline lake passed by evolutionary process, because of the climate and temperature changes in turns, which causing the saline lake deposit ingredients have regular change results.

其中,黑色纹理处盐壳含砂量增加,而白色纹理处以粉和粘为主,明暗相间的环带盐壳颗组成差异是盐湖向干盐湖的演化过程中,由于气候冷暖交替变化而使盐湖沉积物成分发生规律性变化的结果。

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Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

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