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The fragmentation of clarain and culm tends to be severely with the increase of particle size, bed temperature and combustion time.

径越大,炉床温度越高,燃烧时间越长,破碎越剧烈;亮煤与灰煤在燃烧中均发生了二次破碎,其中亮煤的二次破碎更剧烈;燃烧后期,亮煤的颗破碎比灰煤更快;由于破碎,入炉煤颗平均径在燃烧早期迅速减小,而后随着燃烧的进行而逐步趋于一个稳定值;亮煤在流化床燃烧中服从等密度燃烧模式,暗煤服从等直径燃烧模式,而灰煤则服从混合燃烧模式。

After 10 weeks immunization, the combinant plasmids distribute different organs and tissues of the BALB/c mice immunized by DNA vaccines of Toxoplasma gondii ,but only blood was Detected out the recombinant plasmids.

结论弓形虫DNA疫苗免疫BALB/c鼠5周后,多个不同器官组织均可检测出重组质,免疫后10周血液检测出重组质

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中,得到的重组质命名为pGBKT7-Gα,β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性,将其转化到酵母菌pJ69-4A中,再以此为受体菌转化拟南芥绿色营养组织cDNA文库质

The blocking mechanism takes advantage of the fact that a small sequence of this CRISPR locus matches staphylococcal conjugative plasmids, including those that confer antibiotic resistance in MRSA strains.

这一位点的阻断机理是利用了以下事实:CRISPR位点上的一小段序列与金黄葡菌中包括引起抗生素抗性质在内的结合质中的序列互补。

Methods Agarose gel electrophoresis analysis was used to detect plasmid profiles. Plate transconjugation and broth transconjugation were used to examine the conjugative resistant plasmids and the agar plate dilution method was used to analyze the minimum inhibitory concentration of 15 antimicrobial agents.

用改良Kado-Liu法提取细菌质,琼脂糖凝胶电泳检测质,用平板稀释法测定药物最低抑菌浓度,用试管内肉汤接合法和平板表面接合法进行接合转移试验。

These jadeite jade specimens show mainly following texture types: granular crystalloblastic texture, equigranular crystalloblastic texture, inequigranular crystalloblastic texture, prismatic crystalloblastic texture, f...

主要的结构类型有状变晶结构(粗状、中状、细状及微状)、等变晶结构、不等变晶结构、柱状变晶结构、纤维状变晶结构、碎斑结构、交代结构、糜棱结构及碎裂结构等。

The total seed storage in topsoil (0-10 cm) was 83499-109141 indm^(-2) for natural wetlands and 9140-47452 indm^(-2) for reclaimed paddy fields. In natural wetlands, the seeds were of 16-30 grass plant species, being dominant with Polygonaceae and Cyperaceae or Cyperaceae and Gramineae; while in reclaimed paddy fields, the seeds were of 7-16 grass plant species, with the dominant of Gramineae. The seed storage and species numbers were greater in Shengjin Lake than in Shibasuo Lake. Higher seed bank storage and more species were observed in the paddy fields from Shibasuo wetland with 30 years of cultivation than from the Shengjin Lake wetland with over 50 years of cultivation.

结果表明:天然湿地种子库密度为83499~109141m^(-2),而开垦为稻田30~50年后种子库密度为9140~47452m^(-2);天然湿地的土壤种子库物种数为16~30种,以蓼科-莎草科或莎草科-蓼科为优势种,开垦为稻田后物种数下降为7~16种,且趋向于以禾本科为单一优势种;此外,与十八索湿地相比,升金湖湿地有较高的土壤种子库物种数、丰富度和多样性,而开垦年限为30年的十八索稻田土壤种子库物种数和丰富度显著高于开垦年限为50年的升金湖稻田。

CML is cytogenetically marked by the philadelphia chromosome, which originates from a reciprocal translocation between chromosome 9 and 22 and is molecularly marked a chimeric bcr-abl gene, resulting from juxta-positive of the abl proto-oncogene on chromosome 9 with the bcr gene, which is normally located on chromosome 22. The chimeric bcr-abl gene expression an 8. 5kb hybrid mRNA transcript giving rise to a 210-KD fusion protein (P210〓) with increased tyrosine kinase activity. P210〓 plays a key role in the pathogenesis of CML. The continuous cell line K562 was established from the pleural effusion of a 53-year-old female with CML in terminal blast crisis, and was a human erythroleukemia line, contained Ph chromosome.

绝大多数慢患者白血病细胞中具有Ph染色体,是由9号染色体长臂3区4带和22号染色体长臂1区1带相互易位形成,即t(9;22),使位于9q〓的c-abl原癌基因在第二外显子的5'端断裂并易位到22 q〓的M-bcr基因第2或第3外显子的3'端,形成异常的bcr-abl嵌合基因,该基因转导出异常的mRNA,编码并翻译出P210蛋白,该蛋白具有很强的酪氨酸激酶活性,使细胞发生恶性增殖。K562细胞属于慢急变、红白血病细胞株,具有Ph染色体。

In order to do this, we get equal quantities of plasmid pSCA1-M, pcDNAM and pSCA1 transfected vero cells. After 48 hours, we observed the presence of special green fluorescence in cytolymph in both of pSCA1-M and pcDNAM groups. However, this fluorescence is absent in the negative pSCA1 group.

分别取等量的重组质pSCA1-M、常规IBV M基因DNA疫苗质pcDNAM、空载体pSCA1转染Veto细胞。48h后经间接免疫荧光试验观察发现,pSCA1-M以及pcDNAM转染组细胞浆均可见特异性亮绿色荧光,pSCA1空载体组无荧光为阴性。

With electron microscope techniques, the cytopathological changes of the tobacco leaves infected by TMV-W and TMV-O 17 were observed.

受TMV-017侵染的烟草细胞中,叶绿体数量较少,叶绿体具双层膜结构,基片层排列整齐,有的叶绿体膨大,内含少量小的淀粉

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