粒

Methods:The luciferase reporter plasmids of MDM2 P2 promoter containing different SNP309 were contructed,and cotransfected into CV-1 cells with AML1-ETO,AML1 and SP1 expression plasmid.The transactivities of luciferase were assayed by luminometer to determine the impact of AML1,AML1-ETO,and SP1 on MDM2 P2 promoter.

研究方法：构建含不同SNP309位点的MDM2 P2启动子的报告质粒，与AML1-ETO、AML1以及SP1的表达质粒单独或共转染CV-1细胞，测定荧光素酶的活性，分析AML1-ETO、AML1以及SP1对MDM2 P2启动子的影响。

Methods:The expressive plasmids of AML1b and AML1-ETO were transfected into CV-1 and 293 cells.The expression level of endogenous pig7 gene was detected by Realtime-PCR.The luciferase reporter plasmids containing pig7 enhancer/corresponding mutant sequences were constructed and co-transfected into CV-1 cells with expressive plasmids of AML1b and AML1-ETO.The transactivity of pig7 enhancer was assayed by luminometer.

研究方法：AML1b和AML7-ETO表达质粒分别转染及共转染CV-1和293细胞，用Realtime-PCR方法检测上述基因对两种细胞内源性pig7基因mRNA表达水平的影响；构建含AML1b结合位点的pig7基因增强子序列以及相对应的突变位点的荧光素酶报告基因质粒；将表达载体/突变载体与AML1b或AML1-ETO基因瞬时共转染CV-1细胞，分析AML1b、AML1-ETO对报告基因的转录调节作用。

Methods: The residual lymphoblast percentage of bone marrow was evaluated on day 14 during a four-drug induction in 21 children with ALL. Telomerase activity of bone marrow cell was determined by telomere repeat amplification protocol -silver stain technique at differential clinical stages.

以多药联合化疗方案进行诱导缓解和缓解后治疗，以诱导治疗第14天的骨髓原始淋巴细胞比例作为治疗反应敏感性指标，以端粒重复序列扩增-银染色技术检测端粒酶活性作为微小残留病白血病指标。

To prove that the cloned DNA fragment can express tryptopanase,a new plasmid pET28C-TnaA , in which the cloned DNA fragment was located downstream of T7 promoter on pET28c was constructed and transformed into host BL21(DE3),a BL21 lysogen of bacteriophage DE3 in which the only promoter known to direct transcription of the T7 RNA polymerase gene is the lacuvS promoter ,which is inducible by IPTG.

为了证明质粒上的基因能表达出有活性的色氨酸酶，将这个DNA片段克隆到PET28c质粒的BamHI和HindⅢ位点上，使该片段受T7 RNA聚合酶的启动子控制，然后转化噬菌体DE3的溶源菌BL21(DE3)。

It is a contradiction to achieve a high material removal rate and a super smooth surface in lapping of magnetic head.

同时获得高去除率和超光滑平整的表面是硬盘磁头自由磨粒抛光的矛盾，研究自由磨粒抛光材料去除规律可以优化抛光工艺。

The Malvern 2600 Particle Size Analyzer was used to determine the droplets size distribution of a nozzle.

喷嘴之粒径分布资料，使用雷射粒径分析仪(Malvern 2600)予以量测。

Paraffin section technique was applied in the study of the development of the seed coat of Pinus kwangtungensis. The results showed that the development of seed coat follows with the development of the megaspore mother cell. In the development of female gametophyte, a thin, dense and dark layer of cells formats around the female gametophyte.

运用石蜡切片法，在透射电子显微镜和光学显微镜下观察华南五针松种皮的形态结构和发育，用PAS反应显示细胞内淀粉粒分布，研究细胞内淀粉粒的含量。

Coli XL-1 strain, purposed cassette recombination plasmids pNB0098-K and pNB0097-K were constructed, in which the purposed gene was linked with plasmid pUC18, and the marker gene kanamycin was inserted in the purposed gene. The linear cassette DNA fragment was mixed in the medium of cultured competent cells of meningococcus BT878. The function of the purposed gene was inhibited by the homologous replace of casstte gene.

将线性的重组质粒片段置于培养基中，与感受态的脑膜炎双球菌混合培养，重组质粒中的同源性片段被脑膜炎双球菌细胞识别和摄取，整合到染色体上，目的基因被带有卡那霉素基因插入的同源性重组片段代替，导致失活，在选择性培养基上筛选出基因被敲除的突变株。

The introduction of the acrylic or methacrylic acid in middle layer is helpful to obtain a latex with smaller particle size and more narrow particle size distribution.

本文对聚合工艺、乳胶粒中间层分率和软硬单体比例、官能团单体含量等对GAD室温自交联乳液的粒径及分布的影响进行了研究。

The dominant species of fungus are Penicillium, Cladosporium and Aspergillus, and size distribution ranges between 2.1 and 3.3 m. The dominant species of bacteria are Glucose-Non-Fermentation and Micrococcus, and size distribution of the bioaerosols are 0.65 to 1.1 m and 2.1~3.3 m respectively.

真菌的优势菌种为Penicillium， Cladosporium， Aspergillus，大多数粒径分布2.1~3.3 m之间；细菌的优势菌种为Glucose-Non-Fermentation， Micrococcus，大多数粒径分布在0.65~1.1 m及2.1~3.3 m之间。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。