粒

Methods The level of telomerase activation and PCNA expression were detected in 80 cases of breast cancer,36 cases of side-cancer tissues and 10 cases of lacteal gland benign lesion using TRAP-PCR-ELISA and S-P immunohistochemistry technique.

采用端粒重复序列扩增聚合酶链反应-酶联免疫吸附测定法(TRAP-PCR-ELISA)和免疫组织化学链霉素生物蛋白过氧化物酶法对80例乳腺癌组织、36例乳腺癌相应癌旁组织和10例乳腺良性病变的端粒酶活性及增殖细胞核抗原进行检测。

Methods The level of telomerase activation and PCN A expression were detected in 80 cases of breast cancer,36 cases of side-cancer tissues and 10 cases of lacteal gland benign lesion using TRAP-PCR-ELISA and S-P immunohistochemistry technique.

采用端粒重复序列扩增聚合酶链反应-酶联免疫吸附测定法(TRAP-PCR-ELISA)和免疫组织化学链霉素生物蛋白过氧化物酶法对80例乳腺癌组织、36例乳腺癌相应癌旁组织和10例乳腺良性病变的端粒酶活性及增殖细胞核抗原进行检测。

Part two, The consructon of universal probe real time PCR detecting of Avian viral disease We constructed a plasmid including a specified fragment of NDV, and optimizated reactive condition of universal probe real time PCR, and detected Infectious Laryngotracheitis Viruse, Infectious Bronchitis Viruse and

第二部分：通用探针实时荧光PCR检测禽病毒病方法的建立本部分首先构建含有NDV特定片段的质粒，以提取的质粒为模板

The efficiency of surfactant was evaluatedby measurement of the polymerization kinetics, the conversion of MMA, the amount of thecoagulation, the surface tensteon of the miniemulsion before and after polymerization, andthe particle size and particle size distribution of obtained lattice.

通过对聚合反应的动力学，单体MMA的转化率，凝结物的生成量，细乳液聚合前后的表面张力，及所得乳胶粒子的粒径和粒径分布的表征，评价了大分子乳化剂在RAFT细乳液聚合中的效率。

The conversion boundary of self-incompatibility was researched by sowing by stage. Sunshine of day and temperature was studied critically by investigating leafage and researching traits indoors. The results showed that the daily minimum temperature was the principal factor of affinity conversion and the affinity conversion boundary was 16.5~18.1℃.HE97 was primarily regard as self-incompatible line in this research. The method was by inbreeding and sib HE97, Half the silk of HE97 and Zheng58. Inbreeding and cross have been done simultaneously. The extending condition of pollen tube was observed by dying the silk after pollinating 4 hours. The individuals with pollen tube were observed having seeds, while the individuals without pollen tube were observed having few seeds or no seeds. So this study regard HE97 as sporophytic self-incompatibility .The planting ration of female parent HE97 and male parent 87-1 was 4:1.The nature cross-pollination was studied by the theory of endosperm xenity, the study show that the setting percentage of HE97 was 625%, in which the seed in self was 70% and the seed in hybrid was 30%. This was consistent with the study of silk anatomy structure, then come to the same conclusion: compatible pollen germination and fertilization can stimulate the self-incompatible pollen germination and fertilization in the same corn cob.

通过分期播种，进行了光温敏自交不亲和材料HE97亲和性转换区间的研究，依据田间跟踪调查叶龄及室内考种研究了HE97亲和性转换区间的光温临界值，结果表明：1日最低温度是影响HE97自交不亲和性的主要原因，亲和性转换区间在16.5℃～18.1℃之间。2通过HE97自交和姊妹交以及将HE97与郑58作为双亲，各自的花丝一分为二，分别正反交，授粉后4个小时分别取其花丝进行固定、染色，观察花粉管的伸长情况，根据结实率与花粉管伸长的对应关系，结果发现观察到花粉管的对应植株收获到了种子，而没有观察到花粉管的对应植株结实率极低或没有结实，本研究把HE97初步划为孢子体自交不亲和类型。3以HE97为母本，87-1为父本，按4母：1父行比，通过花粉直感效应研究天然异交率，结果母本HE97结实良好(62%)，其中自交粒占70%，异交粒占30%，结合花丝切片观察表明，亲和花粉的萌发及受精会导致落在同一果穗柱头上不亲和花粉的萌发不再受抑制。

Main Outcome Measures We measured leukocyte telomere length at baseline and again after 5 years of follow-up.

研究人员在研究开始的时候测量了这些人的白细胞端粒长度，并在5年的随访之后再次测量了其端粒的长度。

Results The mean size of the paclitaxel liposome and proliposome were 210 nm and 279 nm, respectively.

结果 冻干前紫杉醇脂质体的平均粒径为210 nm，冻干后脂质体的平均粒径为279 nm。

Loam is a mixture of sand, silt, and clay.

壤土是砂粒、粉粒和粘粒的混合物。

So it is assumed that the E-box might be associated with the enhancement of transcription activity.Here, the LTRs of WH17 ,DLA-25,DLA-118 and LTR with single mutation in the E-box motif were separately cloned into pCAT-Basic vector resulting in a series of recombinant plasmids containing CAT reporter under the control of different versions of LTRs.

通过对EIAV分离株WH17的LTR与L株、DA和DLA株的LTR序列进行比较，发现其在在U3-R结合处多一个E-box基序，推测该基序的变化可能会起到促进转录作用，为此将EIAV强毒株(DLA-25)、驴白细胞弱毒株(DLA-118)、EIAV分离株WH17以及U3-R结合处的E-box基序点突变的LTR分别克隆到pCAT3-basic质粒中的报告基因氯霉素乙酰转移酶的上游，获得一系列受不同LTR控制的CAT表达质粒。

Its cDNA had been cloned by RT-PCR. The results of comparison on sequence homology suggested that Luffin P1 may be derived from a vicilin like precursor which is a storage protein in the seeds of Luffa cylindrica.

本文利用3'RACE法克隆了它的cDNA，并构建了它的融合表达质粒pGEX-4T-S2，该质粒在大肠杆菌中获得了成功表达。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。