粒

Preparation of three group of liposome that the mean diameters were 35~65 nm and the entrapment efficiency were 50~80%.

此三组所制备出的微脂粒平均粒径大小为35~65 nm，包埋率在50~80%之间。

Flow cytometric also used for analysis the levels of reactive oxygen species, Ca2+ and mitochondrial membrane potential in both cell lines. The results showed Gyp promoted ROS and Ca2+ production and decreased the MMP level. Western blotting show that Gyp treatment gradually decreased the levels of anti-apoptotic protein (Bcl-2), but increased the levels of the pro-apoptotic protein.

除此之外，也藉由流式细胞仪分析测得经绞股蓝皂甘作用后的人类血癌细胞（HL-60）和小鼠血癌细胞（WEHI-3）里的活性氧化物浓度上升、钙离子释放以及粒线体膜电位下降等细胞凋亡重要指标，此结果可经由西方墨点法指出绞股蓝皂甘能使癌细胞中的抗凋亡蛋白Bcl-2表现量下降；促凋亡蛋白Bax表现量上升，同时也藉由共轭焦显微镜观察到致死蛋白AIF、Endo-G从粒线体释放至细胞核。

Gyp promoted the release of cytochrome c from mitochondia based on the changes of MMP and the activation of caspase-3 before leading to apoptosis. We also used confocal laser microscope to show AIF and Endo-G are released from mitochondrial then move to nuclei

根据上述多种实验结果可证实：绞股蓝皂甘能引起细胞中粒线体膜电位改变导致凋亡蛋白Cytochrome c从粒线体释放出而活化了caspase-3，进而诱发人类血癌细胞株（HL-60）和小鼠血癌细胞株（WEHI-3）走向凋亡路径

Using modern biology research technique and means,plasmid extract and agarose gel electrophoresis of molecular biology level explore test were carried out for coal with not coal department the hereditary property of Thiobacillus ferrooxidans.

利用现代生物学研究方法和手段，对煤系与非煤系氧化亚铁硫杆菌的遗传特性进行了质粒抽提和琼脂糖凝胶电泳等分子生物学水平的基础性探索试验，对于质粒在硫杆菌中普遍存在的观点提出了质疑。

Cutting phaseWhen the protruding peak of holystone surface and coarse raceway surface is contacted, because osculatory area is lesser, the pressure on unit area is greater, below constant pressure action, holystone gets above all of workpiece " a traditional method of indicating the pronunciation of a Chinese character by using two other Chinese characters is cut " action, make the part of holystone surface grinds bead to fall off with disintegrate, show a few new grind bead and blade edge ......

切削阶段磨石表面与粗糙滚道表面的凸峰接触时，由于接触面积较小，单位面积上的压力较大，在一定压力作用下，磨石首先受到工件的"反切削"作用，使磨石表面的部分磨粒脱落和碎裂，露出一些新的锋利的磨粒和刃边。与。。。。。。

The isoform hMAM cDNA consisting of 270 bp, was different from the wild type one of 279 bp due to nine continuous base pair missing. Therefore the translational product of the isoform was seen to be lack of three continuous amino acid (-79~-81, VFM) compared with that of the wild type. The homology of the wild type and the isoform are testified to be 96% in both DNA and amino acid sequences.

乳腺癌组织和乳腺癌细胞株中发现两种hMAM cDNA亚型：hMAM和hMAM，长度分别为279和270bp，二者相差9个连续碱基，其翻译产物相差3个连续氨基酸残基(-79～-81，VFM)，二者基因和氨基酸序列匹配度均为96%。cDNA片段分别插入原核表达质粒pGEX-KG，构建的质粒命名为pGEX-KG/hMAM和pGEX-KG/hMAM。

On the basis of our results,IFN-θnot only has the sequence homology to other typeⅠinterferons,but also matches the primary properties of typeⅠinterferons in antiviral, IFNαR receptor binding and virus inducing,etc.

首先，将PoIFN-α基因克隆入pcDNA3真核表达载体中，筛选阳性克隆后大量抽提质粒，通过脱水化-再水化步骤将重组质粒包裹入阳离子脂质体内制备成核酸佐剂(IFN-adjuvant)。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

Be similar to most malignant neoplasms, the immortalization of TCCB cells is close relationship with telomerase and telomere of the end of chromosome.

与多数恶性肿瘤类似，TCCB细胞的永生化和染色体末端的端粒和端粒酶密切相关。

The protective film decreased the surface roughness, and also increaser the friction coefficient in the process BTA absorbed on SiO2 polishing particle surfaces, which decreased the friction coefficient in the polishing process caused by the particles.

另外，BTA对成SiO2抛光磨粒具有一定的吸附作用，进而对抛光效果产生一定的影响，抛光磨粒表面吸附层的存在会减小抛光过程中的摩擦系数。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。