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In the research, we had contracted express vectors of the four genes, and the vector was transfected into K562 cell, then STI571 was united to each express vector differently.

通过构建这四种基因的真核表达载体,转染入K562细胞,并且将基因克隆治疗与STI571联合应用,了解其对于K562细胞增殖、细胞周期及凋亡的影响,对于慢的研究是一个创新性的尝试,对于指导慢的临床治疗具有深远的意义。

Lividans. Using pIJ903 bearing tsr gene as a vector, the two furthermost fragments of the cluster, 4. 0kb and 1. 5kb in size respectively, were inserted into it. To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. OriT from E coli plasmid RK2 was also incoporated into the vector, therefore, pIJ903 derivatives can be mobilized from E.

在具有硫链丝菌素抗性基因的pIJ903的单—BamHI位点,同向插入了FR-008生物合成基因簇两个最远端的4.0kb和1.5kb片段,并在二者之间插入了可在链霉菌FR-008和变铅青链霉菌中表达的阿泊拉霉素抗性基因,同时也插入了有助于将质引入链霉菌的oriT片段,从而构建出了置换整个基因簇的基因置换质pHZ691。

But, fusain is high in density and in HGI. It is usually concentrated in the coals of heavier and finer parts. Less oxygen functional groups exists in vitrain even if the total oxygen is high. However, more oxygen functional groups is found in fusain though total oxygen is low.

指出,镜煤的密度较低、HGI值较小、易富集于密度较氢的粗径煤中,尽管其总氧含量较高、但是含氧官能团的数量相对较低,丝炭的密度较大、HGI值较高、易富集于较重的细径煤中,其总氧含量尽管较低、但是含氧官能团的数量较高。

And dry Panzhihua fine grained gabbro and fine to medium grained gabbro have experienced deformation regimes of semi brittle flow and plastic flow under 700~900℃.

而细攀枝花辉长岩和中细攀枝花辉长岩从 70 0℃到 90 0℃经历了半脆性流动和塑性流动 2个变形域。

This study provides genetic evidence that SUN1-telomere interaction is essential for telomere dynamic movement and is required for efficient homologous chromosome pairing/synapsis during mammalian gametogenesis.

我们的研究首次从遗传学上证明了SUN1蛋白和端的相互作用对于端在动物生殖细胞减数分裂中的动态变化,高效的同源染色体配对、联会以及哺乳动物的配子发生是必不可少的。

In this paper, the technical conditions of preparing TiO2 ultrafine particle used as photocatalyst by Radio Frequency Plasma Chemical Vapor Deposition is studied, then suggest some methods of controlling the size and crystal type of TiO2 particles. And with the existent equipment prepare TiO2 particle taking on varies size by controlling the flow velocity of Ar as loading gaseity in the system of TiCl4+O2. By characterizing we know the particle prepared is the mix type of rutile and anatase.

本论文探讨了高频等离子体化学气相淀积法制备光催化剂用二氧化钛粉体的工艺要求,提出了一些控制粉体径和晶型的方法,并以现有的实验设备为基础,在TiCl_4+O_2体系中,通过控制载气的流量制备出了不同径的二氧化钛粉体,通过表征可知所制粉体为金红石型和锐钛型的混晶体。

Result: 1.(1) Three strips come to appear in the position of 6.0kb, 5.4kb and 600bp after gelose electrophoresis and their size accord with recombined plasmid DNA, pcDNA3 plasmid and VEGF165 gene accordingly.(2) Pure degree of the pcDNA3-VEGF165 recombined plasmid is: OD260/OD280=1.87. Its

在进行了重组质的扩增、提取、纯化、鉴定、兔MSCs的原代及传代培养以及RT-PCR、Western Blot 转染检测后,实验取得了令人满意的结果,证实了MSCs 能在体外有效转录外源VEGF 基因并分泌出VEGF 蛋白,同时也说明pcDNA3-VEGF165重组真核表达质是有效的。

Hairy roots were induced from tobacco leaves with Agrobacterium rhizogenes LBA1334 harboring agropin type PRil855 and binary vector PIG121HM carrying a kanamycin resistant gene nptll and GUS gene and the good hairy root clones were selected. The root with many root hairs and branches grew vigorously toward all the direction on MS medium without plant hormone and on kanamyain containing medium (km:30mg/L). The structure of the hairy root tip is different from that of ordinary root. Witch was not typical root cap maybe the main cause of losing the geotropism of hairy root. Plantlet regenerated from hairy root when cultured on Ms medium without plant hormone. The nicotine content of the hairy root was little higher than that in the natural roots.

本研究通过发根农杆菌工程菌LBA1334(含有野生型pRi1855质和pIG121HM双元载体质,编码NPTⅡ基因和GUS基因)转化烟草沙姆逊,获得了具有卡那霉素抗性烟草发状根,并筛选出良好的单克隆株系,该发状根具有典型的发状根特性(多根毛、多分支、在无激素培养基上快速生长),通过根尖压片分析,烟草发状根不具备典型的根冠;发状根中烟碱含量1.61%稍高于与天然栽培品种母体根的含量(1.5%左右),发状根向培养液中释放烟碱,释放量最多时占总烟碱量的81.1%;在无激素的培养基上获得了再生植株。

Textural properties of feed (fiber content, particle size and particle integrity) are important for proper gizzard musculature and motility.

饲料的结构特性(纤维含量、径大小和的完整性)对肌胃的肌肉组织和运动是很重要的。

The main products are: Crystal full grain leather, soft full grain leather, shine skin, waxing and other skin.

主要产品有:水晶全面革、软全面革、油光皮、打腊皮等。

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