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BALB/c mice were immunized with plasmid TR421-hCGβ or mock DNA three times. Two weeks after immunization, spleen cells from the immunized mice were harvested, and then analyze the CTL activity against Sp2/0-ehCGβ cells. The splenocytes derived from the immunized mice were adoptively transferred to the normal mice, which were subsequently given injections i.

以TR421-hCGβ质实施基因免疫,免疫后检测小鼠脾细胞,特异性细胞毒活性;同期将脾细胞过继免疫给正常小鼠,以过继TR421-hCGβ质免疫小鼠脾细胞为实验组、过继TR421质免疫小鼠脾细胞为对照组,检测脾细胞杀伤效应。

Shortening of telomere may be one of pathomechanism of spleen Qi deficiency syndrome. The accumulation of free radical produced by the decrease of the antioxygen ability may be damage the telomere, and that may be one of the mechanisms of telomere shortening in liver of rat model with spleen Qi deficiency syndrome.

提示端长度缩短可能是脾气虚证的病理机制之一;脾气虚证状态下,机体的抗氧化能力下降、自由基蓄积损伤端可能是脾气虚证模型组大鼠肝组织端长度缩短的机制之一。

The concentration of chlorophyll a was higher in the aquacultural area,and had no distinct seasonal changes,whereas...

调查海区表层海水叶绿素a含量在近岸养殖区较高,季节变化不明显,但其级结构有较大的季节差异;初级生产力的平面分布与叶绿素a含量在春、秋季均较一致,其级结构与叶绿素a的级结构在春季基本一致,但在秋季有较大差异。

AF297709 , which has specific interaction with the promoter region of hTERT, was first screened out of the HeLa cells cDNA library with the method of yeast one-hybrid assary in 2000.It was found that TRAP has positive activation effect on the transcription starting of hTERT.

人端酶催化亚单位hTERT组分的水平决定了细胞中端酶的活性;hTERT表达是端酶激活的关键步骤,一些转录因子、肿瘤抑制因子、细胞周期抑制因子、细胞寿命决定因子、激素受体和病毒蛋白参与了hTERT表达调控。

Results: The light microscope pictures of group A(no [α-32P] ATP in the perfusate) showed that there was no specially labeled silver pellet in or out of rat liver cell. But the light microscope pictures of group B (37 MBq [α- 32P]ATP in the perfusate) showed that distribution of numerous [α-32P]ATP autoradiographic silver pellets in rat liver cell, and no autoradiographic silver pellet was found in the hepatic sinus and vessel.

结果:灌流液中不加[α-32P]ATP的A组光镜照片显示大鼠肝细胞内外均未见特异性标记银显影,而灌流液中含37 MBq[α-32P]ATP的B组光镜照片显示大鼠肝细胞内可见多个大小不等的[α-32P]ATP自显影银分布,而肝窦及肝小叶中央静脉等血管内则几乎无黑色自显影银分布。

Effect of Different Sowing Date and Densities to the Yield of Avena nuda L .Variety Huazao No.2;2. The paper analyses the chromosome karyotype of Avena nuda L .

对莜麦染色体的核型进行了分析,结果表明其染色体数为42,为六倍体,包括11对中部着丝染色体,9对近中部着丝染色体和1对近端着丝染色体,其中有3对染色体带有随体。

A small fraction of bacterial chromosomes (such as those in Streptomyces and Borrelia) is linear and possess telomeres, which are very different from those of the eukaryotic chromosomes in structure and functions.

一小部分细菌的染色体(如链霉菌属和疏螺旋体属细菌的染色体)是线性的并且具有端,但是它们的端与其他的真核生物的端在结构和功能上有很大的区别。

As for the radius of porous media effect our experiments select four kinds of sea-sand whose pore radius vary between 125-700μm.The results shows that the pore radius we use in the experiments are too large to produce capillary effect which can clag the process of hydrate formation, only if the pore radius is smaller than 60 nm the can produce capillary effect.

基于径范围125-700μm的四种多孔介质的甲烷水合物生成实验结果表明,较粗径的多孔介质引起的孔隙间毛细作用对甲烷水合物不会产生明显的阻碍作用,但计算结果表明,孔径径小于60nm的多孔介质会阻碍甲烷水合物的生成。

Then the combinant plasmid was conducted into ACC-2 cell by electroporation. ACC-2 cells stably expressing CD was obtained by 10-day positive selection with 400 μg/mL G418. Total RNA was extracted and the expression of the CD gene in transfected ACC-2 cells was identified by RT-PCR. RESULTS: PCR yielded a fragment of l280bp and CD was verified by sequence analysis.

测序正确后,将其亚克隆到质表达载体pIRES中,构建以内部核糖体进入位点相连的CD基因的质表达载体pIRES-CD,采用电穿孔法,以质表达载体转染ACC-2细胞,用400μg/mL的G418筛选10d,获得稳定表达CD基因的ACC-2细胞系,提取该细胞的总RNA,用RT-PCR检测CD基因的表达。

Elimination of conjugative drug resistance plasmids and nonconjugative drug resistanceplasmids from 20 and 19 Shigella strains respectively by norfloxacin and berberine in vitro were analysed.

用氟哌酸和黄连素对20株携有接合传递耐药性质及19株携有非接合传递耐药性质志贺氏菌进行体外消除研究。结果显示,可被消除的耐药性质大多数在24小时内被消除。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。