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The structure gene pelA from Thermotoga maritima MSB8 encoding pectate lyase was amplified and ligated into pHsh, resulting pHsh-pelA. Through structural optimization on pHsh-pelA, the ultimate plasmid, pHsh-pelC, which possessed the most appropriate structure and free energy of mRNA, was obtained.

将来源于极端嗜热菌属海栖热袍杆菌Thermotoga maritima MSB8的编码碱性果胶裂解酶的结构基因pelA与新型热激质pHsh连接,得到重组质pHsh-pelA,运用mRNA二级结构预测软件对pHsh-pelA的翻译起始区的二级结构进行优化,得到了具有最佳mRNA二级结构及自由能的质pHsh-pelC。

A chemical used to render visible the image recorded on a photosensitive surface.

小麦一种硬麦(硬小麦原为粗麦小麦属),主要用于制作意大利面食

The function of hypothesis transport genes NMB0098 and NMB0097 were tried to study by way of gene knock out. The phototype of N. meningitidis strain BT878 was observed before and after gene NMB0098 was knocked out.

选用质pUC18为目的基因的载体,经过酶切、去磷酸化、连接、电穿孔法转导和抗生素筛选等步骤,分别将目的基因NMB0098和NMB0097与质相连,并在目的基因中插入标记基因—卡那霉素基因,构建盒式插入的重组质pNB0098-K和pNB0097-K。

The variation of grain weight under different cultural and environmental treatments was essentially determined by the hull size, and that of grain weight among different specific gravities was essentially determined by grain plumpness, that were found to be consistent in the four varieties.

在不同栽培和环境条件下,谷壳大小是造成处理间重差异的主要原因,不同类型品种表现一致;在籽不同比重条件下,谷充实度高低是造成不同比重等级谷重差异的主要原因,不同类型品种表现一致。

Laboratory is with diameter of fine pink bead - 80+160 looks (all the grain of polyvinyl chloride fine pink that bead diameter is 138 μ M), thick grain is all the polystyrene grain that bead diameter is 1.1 Mm.

实验所用细粉径为-80+160目(均径为138μm)的聚氯乙烯细粉颗,粗颗为均径为1.1 mm的聚苯乙烯颗

However, there are still several safety concerns about the use of a DNA vaccine, which include the possibility of integration into the host genome, adverse immunopathology, and anti-DNA autoantibody induction.Attempting to solve some of these problems, eukaryotic expression plasmids pTL-8/ AMA-1 and pTL-8/ AMA-1 which express trans-activator or reverse trans-activator, respectively, and AMA-1 gene of Plasmodium falciparum were constructed by using tetracycline regulable system.

首先构建了恶性疟原虫顶第四军医大学硕士学位论文端膜抗原1(AMA一1)基因和反式活化因子基因的真核表达质pTL一8/灿认一l和PTL一8/灿认一1,并大量制备这两种质及表达转录抑制子的质pUHS6一1。

It shows that the all physical mechanical index of small size spall satisfy the design demand except the grain composition.

通过对小径石料室内及现场试验研究,小径石料填筑混凝土面板坝堆石体的应力应变分析以及大坝变形观测分析,认为,小径石料的物理力学性能指标除了级配不满足设计规范外,其他指标均满足设计要求。

The results showed the morphology of AMMN was mostly spherical and well-distributed, the mean diameter of the nanoparticles was 15 nm and the diameter ranged from 10~20 am and its Zeta potential was about +29.3 mV. These nanoparticles had tremendous durability in acid solution at pH 2.0 while the untreated particles are corroded in acid solution.

结果显示,精氨酸修饰的磁性纳米颗均匀,分散性好,大多呈圆球型,平均径为15 nm,径范围10~20 nm,其Zeta电位为+29.3 mV;多层修饰的纳米磁在pH 2.0的酸中具有很好的耐酸性能,而未处理的纳米子在酸溶液中发生了溶解。

More than 85% of initial VEGF contents were eat rapped into The VEGF nanoparticles. The result of election micrograph and The size distribution showed that the VEGF nanoparticles acre small spherical or similar spherical vesicles. The mean, diameter of VEGF nanoparticles was 90 nm with the range of 50~150nm, and the drug loading was 42%. The redipersibility of freeze drying powder was very good.

结果:制备了纳米胶体溶液及其冻干品:电子透射显微镜下观察纳米形态,外形为径比较均匀的球状或近球状的纳米,平均径为90nm,径范围为50~150nm;包封率大于85%,载药量约为42%,冻干品的再分散性良好。

Telomerase reverse transcriptase is the catalytic subunit of telomerase, in the activation of telomerase play a key role.

酶逆转录酶是端酶的催化亚单位,在端酶的激活中起关键作用。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。