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Firstly, the pGL3-Control vector was reconstructed , the pGL3-Enhancer vector deletious of SV40 promoter gene was obtained . Then the IFN-β promoter gene was cloned into the pGL3-Enhancer vector and pGL-IP21, the Luciferase reporter plasmid with IFN-β promoter was OK. The availability of pGL-IP21 was verified by NDV ,the inductor of IFN-β, the Luciferase activity was assayed in cells transfected with pGL-IP21 by Luminometer.

首先将pGL3-Control载体进行了改构,除去了SV40启动子基因,获得了pGL3-Enhancer载体,将获得的IFN-β启动子基因连入载体中,构建了带有人IFN-β启动子基因的荧光素酶报告质IP-21,并且通过实验证明所构建的质在干扰素的诱导剂NDV的作用下能够表达荧光素酶活性,照度计检测荧光素酶活性增强,从而验证了所构建的重组质的有效性。

Our aim was to investigate the effect of malate and GABA on telomerase activity of Hela cells and ex-plore the relationship of telomerase activity change with growth, metabolization and deterioration of cancer cells to provide new tactics for treatment of malignant tumor.

Hela细胞是研究端酶的常用细胞,本研究旨在观察苹果酸和GABA这两种作用因素对Hela细胞端酶活性的影响,探讨端酶活性的变化与二者在肿瘤细胞生长、代谢和恶化方面作用关系,为恶性肿瘤的治疗提供新的策略。

Purpose : To study the relationship of the telomere length and telomerase activity to immortalization or malignant t ransformation of the immortalized esophageal epithelial cell line and malignantly t ransformed cell line induced by E6 and E7 genes of HPV 18 type.

目的:探讨端长度、端酶活性以及端酶亚单位组分的表达与食管上皮细胞永生化和恶性转化之间的关系。

Objective To evaluate the helical CT features of miliary tuberculosis and pulmonary miliary metastatic nodules so thatimproving the correct diagnosis of the miliary lung diseases.

方法收集经临床证实的34例粟型肺结核和54例粟性肺转移瘤的螺旋CT资料,观察肺粟结节的分布、大小,肺密度,肺门、纵隔淋巴结增大及肺内并发病灶。

Ikey went behind the prescription desk. There he crushed to a powder two soluble tablets, each containing a quarter of a grain of morphia.

艾奇走到处方桌后,把两可溶的药片碾成粉末,每药片都含有四分之一的吗啡,再往药粉里加了点奶糖以增药粉的分量,然后把这混合物熟练地包进一张白纸里。

In order to develop a safe and effective immunoadjuvant to enhance the immunity and resistance of animals against infection, a novel CpG Oligodeoxynucleotides containing 11 CpG motifs was synthesized and inserted into the VR1012 plasmid, designated as VR1C. Then the recombinant VR1C was entrapped with Chitosan nanoparticles prepared by the method of ionic cross linkage, and employed to inject muscularly 3-weeks old Kunming mice; the blank VR1012 packed with CNP and saline were used to inoculate mice as the control groups. 28 days after inoculation, all mice were orally fed with 0.4ml 2x108CFU/ per mouse virulent hemorrhagic enteritis E. coli to challenge the resistance against infection.

为研制安全高效免疫调节剂增强动物免疫抗病能力,本实验设计合成含11个C pG基序的寡聚核苷酸,重组构建含CpG的VR1012质(VR1C);制备壳聚糖纳米颗包裹重组质(CNP-VR1C),肌注接种3周龄昆明小白鼠,设壳聚糖包裹空质和生理盐水对照组;接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力,Sandwich ELISA测定血清免疫球蛋白和白细胞介素含量。

Aiming at copper cable nigrescence problems in process of producing cable by using white PVC colloid particle,the PVC partiles was tested by TGA ,DSC, TGA -FTIR.

针对使用白色聚氯乙烯胶生产的铜质电缆线氧化变黑问题,对PVC胶进行了热重分析、差示扫描量热分析、热重-红外光谱联用分析等一系列测试,分析PVC胶在规定的加工条件下是否会引起铜质电缆线发黑。

We discovered 460~500 nm line virus and 300 nm bacilliform virus in plant by electronic microscope. They size accord with Odontoglossum ringspot virus and Cymbidium mosaic virus.

感病文心兰和大花惠兰样品在电镜下可观察到长约460~500 nm的线状病毒体和长约300 nm的杆状病毒体,它们分别与已报道的建兰花叶病毒和齿兰环斑病毒体大小一致。

SEM results indicate that the diameter of monodisperse polystyrene latex particles is 317nm, PS latex particles array orderly on the colloidal crystal balls, and surface of inverse opaline balls presents ordered pores.

结果表明,改性PS乳胶呈单分散性,径为317nm;肢体晶体微球表面PS乳胶排列有序;有序大孔SiO2微球表面呈有序多孔,其孔呈六边形,孔径分布均一,约为200nm。

Ordered macroporous SiO2 balls, exhibiting spherical structure with 200nm hexagonal-pores, have been successfully obtained by sol-gel templating method using the colloidal crystal balls consisting of the monodisperse modified polystyrene latex particles, which have been assembled into polymer opaline balls.

采用乳液聚合法合成了单分散改性聚苯乙烯乳胶,利用PS乳胶自组装制得胶体晶体微球,通过溶胶-凝胶模板法制备了有序大孔SiO2微球,通过SEM对改性PS乳胶、胶体晶体微球和有序大孔SiO2微球表面形貌进行了表征。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。