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By means of experiments of marker transfer,acridine orange sensitivity test,F' curring and mini-chromosome transformation it is.conchi-ded that the F' plasmid is always in an integrated state in the Sin strains and that the initiation of the replication of the bacterial chromosome is carrie...

标记转移、吖啶橙敏感性,F′质消除和mini-染色体质转化等实验说明,Sin菌株中F′质始终处于整合状态,并且在40℃中细菌染色体的复制由整合状态的F′质所带动。

By means of experim ents of marker transfer,acridine orange sensitivity test,F' curring and mini-chr omosome transformation it is concluded that the F' plasmid is always in an integ rated state in the SIn strains and that the initiation of the replication of the bacterial chromosome is carried on by the integrated F' plasmid.

标记转移、吖啶橙敏感性、F′质消除和mini-染色体质转化等实验说明,Sin菌株中F′质始终处于整合状态,并且在40℃中细菌染色体的复制由整合状态的F′质所带动。

The deep-seated xenoliths entrained by the Cenozoic Hannuoba basalts can be divided into three groups according to their mineral assemblages:Ⅰ Granulites, including felsic granulites, noritic granulites, and dominantly, two-pyroxene granulites,Ⅱ Pyroxenites, including websterites, spinel pyroxenites, and garnet pyroxenites;Ⅲ Peridotites, including spinel lherzolites and spinelgarnet lherzolites.

根据新生代汉诺坝玄武岩中深源岩石捕掳体的矿物组合类型,将其分为主要的三大类:麻岩类中包括长英质麻岩、苏长质麻岩和二辉麻岩,辉石岩类包括二辉石岩、尖晶石辉石岩和石榴子石辉石岩,橄榄岩类则包括尖晶石二辉橄榄岩和尖晶石-石榴子石二辉橄榄岩。

They are redesigning the seal form of the bearing seat of granulation wheel and adding swing water ring out of the bearing seat,then lock question of the granulation wheel was solved;Enlarging the cooling area of the granulation wheel 's crust to reduce the its distortion;choosing wearable pump to reduce the malfunction.

重新设计化轮轴承的密封型式,并在轴承座外增加甩水环,解决了化轮卡死的问题;扩大了化轮外壳的冷却面积,减少了化轮外壳的变形;改用耐磨泵减小了泵的故障。

Chromosome number in all counted complete metaphases of Carassius auratus var. Dongtingking is 48. Analysis of chromosome can be divided into four sections according to the position of the centromere.

核型分析结果表明,翘嘴鲌的肾细胞染色体组是由48条染色体组成,染色体组型按着丝位置可分为四组,分别是中部着丝染色体、亚中部着丝染色体、端部着丝染色体。

The morphology of virions seems to not be change after incubated with the paeonol by electron microscope. The regained infectivity of TMV recovered by dialysis from incubation with paeonol in vitro showed that the paeonol have not direct viricidal effect. The paeonol did not reduce infectivity when applied before TMV inoculation. The paeonol in low concentration can restrain the polymerization of TMV coat protein in vitro. The paeonol did not reduced infectivity of TMV-RNA in vitro. It is proposed that the paeonol inhibited TMV by binding with TMV coat protein to interfere with recognition of a host receptor essential for infection or by interfering TMV-CP polymerization in plants. The anti-TMV activity of low molecular weight chemicals extracted from Polygonum cuspidatum were determined.

丹皮酚与TMV混合,电镜下观察对体形态没有影响;TMV与丹皮酚混和后透析可恢复侵染力,表明丹皮酚对病毒体并没有直接毒害作用;预先用丹皮酚喷施叶面不影响病毒的侵染,表明丹皮酚未干扰TMV对叶片侵染位点的识别;丹皮酚在低浓度时可阻碍TMV-CP的体外聚合,对TMV-RNA并无体外抑制侵染作用,据此推测丹皮酚对TMV的抑制作用可能主要通过其与TMV-CP结合影响病毒体对叶片表面侵染位点的识别,或影响体内TMV体的脱衣壳或TMV-CP亚基的体内聚合。

The 329 bp terminal inverted repeat sequence can't form the conserved fold-back secondary structure as that of many Streptomyces linear replicons. Lacking of typical Streptomyces tap/tpg locus for te-lomere replication, pPR2.3c encodes a protein with two domains resembling the telomere associated protein of Strepto-myces and helicase of Haemophilus respectively. No typical Streptomyces iteron-rep locus for replication from the cen-trally located origin, two DNA fragments containing almost all pPR2 were cloned and introduced by transformation into S.

其端末端反向重复序列的长度为329 bp,不能像多数链霉菌的线型质那样能形成保守的&折返&的二级结构。pPR2虽然没有参与链霉菌端复制的保守的tap/tpg基因,但是pPR2.3c基因编码了一个双结构域蛋白,分别同链霉菌的端复制相关蛋白Tap和嗜血杆菌的解旋酶具有相似性。pPR2缺少典型的链霉菌重复序列-复制基因区段,将几乎覆盖全长pPR2的两段DNA进行克隆后,不能转化变铅青链霉菌。

Results were shown as followings:(1) Sodium selenite at 0~2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5~2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下:(1)0~2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力;而对SMMC-7721细胞的作用不显著;(2)该浓度硒显著性提高L-02细胞端酶活性、增强hTERT基因表达和延长细胞端长度;而对SMMC-7721细胞的作用均不显著;(3)高浓度硒(5μmol/L以上)显著性抑制L-02细胞生长、致细胞过氧化,灯盏花素能拮抗硒所致过氧化、降低硒毒性;(4)0.5~2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧,同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著;(5)富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白,其结构和功能有待研究;(6)通过已有的含hTERT基因质,成功构建hTERT反义RNA表达质,转染SMMC-7721细胞后对其生长具有抑制作用。

It has been found that the process parameters, the spindle speed, the ultrasonic vibration power as well as the gap between the magnetic pole and the work piece surface, were the key factors in deciding the finishing quality and efficiency of the UMAF.

在研究超声磁复合研磨工作原理基础上,结合传统磁研磨材料去除数学模型,探讨了超声振动对提高磁研磨效率的积极作用,揭示出主轴转速、供磁电流、加工间隙、超声功率等是影响超声磁复合研磨质量和效率的关键因素。

As a key regulator of grain number, the DEP1 locus (and perhaps also its homologs in other small-grain cereals) may provide a means of manipulating grain yield in these crucial crops.

作为谷数量的一个主要调节者, DEP1位点(也可能其他的小作物中与 DEP1相当或相同的结构)可能提供了一个调控主要农作物中谷生产量的方法。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。