类黄酮

There are 65 species of Chinese herbs with confirmed anti-HBV activityd, in which 16 probable active constituents are pointed out. They are arene, ketone derivatives, alicyclic carboxylic acid, aromatic carboxylic acid, ester, lactone, cyanide derivatives, heterocycle derivatives, carotenoids, anthraquinones, flavonoids, vegetable tannin, alkaloid, terpenoids, steroids and protein. Among them, the complexes of flavonoids, terpenoids, steroids and alkaloids are more abundant.

确定了具有抗乙肝病毒活性的中草药品种65种，其中含可能活性成分共16种，即芳烃、酮类衍生物、脂环族羧酸、芳香族羧酸、酯、内酯、氰类衍生物、杂环衍生物、类胡萝卜素、蒽醌类、黄酮类、植物鞣质、生物碱、萜类、甾类和蛋白质，而16种中以黄酮类、萜类、甾类和生物碱类成分为多。

The pigment in colored cotton fibers was extracted by methanol at room temperature. The results of color reactions HCl-Mg reaction, NaBH4 reaction, NaOH reaction, FeCl3 reaction and Pb(Ac2 reaction of the pigment extractive showed that the pigment extracted by methanol was flavone or flavonol with two adjacent hydroxide groups (Table 2, Fig. 1). The results of UV spectral analysis further proved that the pigment extracted by methanol from brown fiber belonged to flavone with two adjacent hydroxide groups in B ring, and that from the green fiber contained not only flavone but also flavonols with two adjacent hydroxide groups both in A ring and B ring and indican replaced a hydroxide group at 3-site of A ring Fig.

通过盐酸－镁粉反应、四氢硼钠还原反应、碱反应、醋酸铅反应、氯化铁反应和紫外光谱吸收的分析表明，甲醇室温提取的棕色棉纤维色素可能是黄酮类化合物，B环上带有邻二酚羟基；甲醇室温提取的绿色棉纤维色素除黄酮类化合物外，还含有3-位羟基被糖甙化的黄酮醇类化合物，A、B环上都带有邻二酚羟基。

To further study the chemical constitutes of lipophilic active parts, we prepared Alc95% and Alc50% according to original literature. 20 compounds were isolated by using macroporous resin, silica gel, polyamide, Sephadex LH-20, Rp-18, preparative TLC and preparative HPLC chromato-graphy. The structures of them were elucidated by means of physical and chemical properties, modern spectroscopic analysis techniques—UV、IR、 EI/MS、ESI/MS、〓H-NMR、〓C-NMR、DEPT、HMQC、HMBC. They are nine anthraquinones: chrysophanol (95-1), physcion (95-2), emodin (95-5), aloemodin (95-7), rhein (50-1), sennoside A or B (50-6),ω-hydroxylemodin (95-10), chrysophanol-8-0-β-D-glucoside (95-12), aloemodin-ω-0-β-D-glucoside (95-13); three phenanthaquinones: tanshinone ⅡA (95-3), cryptotanshinone (95-6), tanshinone Ⅰ(95-4); one isoflavone: 7, 3'-dihydroxyl-5' methoxyisoflavone (95-14) and protocaechuic aldehyde (95-9), protocaechuic acid (95-11), astragaloside Ⅳ(50-4), amygdalin (50-5), 2, 3, 5, 4'-tetrahydroxylstilbene-2-0-β-D-glucoside (50-3),-catechin (50-2), cinnamic acid (95-8). Among them, 3'-dihydroxyl-5' methoxyisoflavone (95-14) is a new compound.

经过溶剂萃取、大孔吸附树脂富集、Sephadex LH-20精制、制备TLC及Semi-pre HPLC等手段从中分离了20个化合物，利用理化性质和波谱技术(UV、IR、EI／MS、ESI／MS、〓H-NMR、〓C-NMR、DEPT、HMQC、HMBC)相结合鉴定了它们的结构，分别为9种蒽醌类化合物：大黄酚，大黄素甲醚，大黄素，芦荟大黄素，大黄酸，番泻苷A或B，ω-羟基大黄素(ω-hydroxylemodin)，大黄酚-8-0-β-D-葡萄糖苷(chrysophanol-8-0-β-D-glucoside)，芦荟大黄素-ω-0-β-D-葡萄糖苷(aloemodin-ω-0-β-D-glucoside)；3种菲醌类化合物：丹参酮ⅡA(tanshinoneⅡA)，隐丹参酮，丹参酮Ⅰ(tanshinone Ⅰ)；1种异黄酮：7，3'-二羟基-5'-甲氧基异黄酮（7，3'-dihydroxy-5' methoxyisofla-vone）以及原儿茶醛，原儿茶酸，黄芪甲苷(astragaloside Ⅳ)，苦杏仁苷，2，3，5，4'-四羟基二苯乙烯-2-0-β-D-葡萄糖苷（2，3，5，4'-tetrahydroxylstilbene-2-O-β-D-g1ucoside），Z—儿茶素，桂皮酸。

In this thesis,with bioassay results in vitro and total contents of flavones as evaluate indexes, the optimal extract method and extract procedure of Bidens parviflora Willd.were firstly studied.

在黄酮类化合物中，又以黄酮醇和黄酮的活性最好，查尔酮、二氢黄酮、橙酮的活性次之，黄酮苷类化合物的活性较弱。

The results of HPLC tests indicated that Bt toxin in Bt transgenic cotton had not obviously affected the contents of flavonoids (mainly rutin and isoquercitrin and a few quercetin ),contradictively ,the result of combined dosage test in vitro showed that that rutin had significantly enhanced the suppression of Bt-ICP on the cotton bollworm larvae., in addition to the

采用高效液相色谱法测定转Bt基因棉花主要抗虫黄酮类化合物的的变化，结果表明外源Bt杀虫蛋白表达不会对棉花自身原有的主要抗虫黄酮类物质（包括芸香苷、异槲皮苷和含量较少的槲皮素）产生不利的影响，因此可以将Bt杀虫蛋白基因转入抗虫黄酮类物质中国农业科学院博士学位论文含量高的棉花品系。

In addition, a pre-column derivatization HPLC method was applied to the determination of Astragaloside Ⅳ; the flavonoids was determined by full-range HPLC scanning; a colorimetric method was developed for the determination of flavonoids and saponins. Hierarchical cluster analysis was performed on three constituents of different Radix Astragali species, and its result was compared with those of traditional phytoecological identification and the pharmacological quality assessment.

本研究以比色法测定了黄芪中总皂苷的含量，以柱前衍生化高效液相色谱法测定了黄芪中黄芪甲苷的含量；以比色法测定了黄芪中总黄酮成分的含量，以反相高效液相色谱法测定了黄芪中毛蕊异黄酮和毛蕊异黄酮苷的含量；以黄芪中的黄芪甲苷、毛蕊异黄酮和毛蕊异黄酮苷为主要特征对不同品种的黄芪进行了系统聚类分析，并将聚类结果与传统生药学鉴定和药理质量评价结果进行了比较。

In leaf tissue of Arabidopsis, the most abundant hydroxycinnamic acidphenolics are sinapate esters, and the most abundant flavonoids are kaempferol derivatives.

拟南芥药用成分主要有酚类化合物，即羟基苯丙烯酸酚类化合物的芥子酸酯，黄酮类化合物的4，5，7-三羟基黄酮衍生物，芥子酸酯与这种黄酮类化合物具有优良的减少紫外线危害和抗氧化作用。

The main purpose of this thesis was the chemistry composition analysis and research of Sorbus tianschanica . This thesis first made preparation of an experiment in detail to the fruit of the sorbus tianschanica to Xinjiang ,then mainly aimed to research flavonoid compounds of the sorbus tianschanica.

本论文主要对新疆天山花楸的黄酮类化合物进行了研究，首先对天山花楸的果实进行了预实验，然后对天山花楸枝叶的黄酮类化合物进行了定性研究及对其不同有效部位的黄酮类化合物进行了较为详细的定量研究。

It is not known whether flavonoids can be absorbed in amounts sufficient to exert an antiviral effect in humans, and therefore their possible role in the treatment of childhood exanthems remains unknown.

约黄酮类化合物抗病毒行动已观察到的试管实验。41 42 43 44 45 槲皮素，黄酮类化合物之一的，所表现出特别强烈的试管抗病毒特性； 46 47 48但是，一项研究没有找到槲皮素将有利于小鼠的病毒感染。49这不知是否黄酮类化合物可吸收的数额不足以在人体发挥抗病毒效果，因此，他们在治疗儿童exanthems可能发挥的作用仍然不得而知。

Several methods were selected to determine the content of flavonoids in corn silk, while satisfying wave length wasdecided by means of UV-visible photometer curves of flavonoids and its monomer in corn silk, as well as rutin at the range of200~600nm after reacting with different regents , and comparison of experimental results were carried out.

本文选用多种黄酮类测定方法，通过对玉米须黄酮类提取液、单体及芦丁标样用不同的显色剂反应后，测定各反应液在200~600nm范围内的吸收曲线，确定了适合玉米须黄酮类定量测定的波长，并对不同测定方法的定量结果进行了比较。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。