类酶

UV-B-induced damage in such leaves could be reduced with 0.5 mmol.L-1 NaHSO3 treatment; brown spots on the leaf surface were reduced in number, the degree of leaf wilt was reduced, degradation of chlorophyll and carotinoid was reduced and flavonoid level was increased. With the NaHSO3 treatment, the content of H2O2 in leaves of Phaseolus vulgaris under enhanc ed UV-B radiation was significantly lower than in controls, and the activities of antioxidant enzymes were higher with treatment than in controls.

结果表明：与未处理对照相比较，用0.5 mmol.L-1 NaHSO3处理的离体红芸豆叶片表面褐色斑减少、边缘蜷曲及萎蔫程度降低；且能延缓叶片中叶绿素和类胡萝卜素含量的降低；使类黄酮含量升高；叶片中过氧化物酶和抗坏血酸过氧化物酶活性升高，过氧化氢(H2O2)含量降低。

Polyphenols can not be combined sufficiently the activity of enzyme decreased slowly because of insufficient cellular fragmentation in the processing of blacke tea congou. But the activity of hydrolydase will increase with the damnification of cell. So on the contrary the activity of P -glucosidase continues increasing.

工夫红茶加工过程中，揉捻阶段叶细胞并未充分破碎，使多酚类物质与酶的结合不充分，酶活性下降较少，但细胞损伤，水解酶活性会升高，因而最终结果β-葡萄糖苷酶活性反而继续上升，致使发酵阶段酶活性大于红碎茶，为工夫红茶香气的形成奠定了基础。

In order to explore the mechanism of the resistance to cucumber damping-off induced by plant growth-promoting rhizobacteria CH1, the physiological and biochemical method was employed to study the changes in activities of Phenylalanine ammonia-lyase, Polyphenol oxidase and contents of ferulic acid, chlorogenic acid, lignin, phenolic compound and flavonoid of phenolic metabolism in cucumber roots pretreated with plant growth-promoting rhizobacteria CH1 and infected by Pythium aphanidermatum.

为研究植物生长促生细菌CH1诱导黄瓜对猝倒病的抗性的作用机制，采用生理生化的方法，测定了黄瓜根部酚类物质代谢中苯丙氨酸解氨酶、多酚氧化酶活性动态及阿魏酸、绿原酸、木质素、总酚、类黄酮含量变化。

There are two kinds according to reaction mechanism,they are depolymerizing Enzyme and Pectinesterase.Peclinase are generally discovered in fruitage and microorganism. It was widely used to defecation procession of juice in food industry, procession being retted of flax in spinning industry and feedingstuff additive in farming.

按其作用机理分为果胶解聚酶和果胶酯酶两大类；它普遍存在于植物果实和微生物中，广泛应用于食品工业的果汁澄清加工，纺织工业麻类脱胶，畜牧业的饲料添加剂等。

The zymogramatic variations of esterase isoenzyme and superoxide dismutase isoenzyme of 20 sample materials, including 5 species, 2 varieties, 1 multiploid of Isatis L. and 1 outgroup species, Coronopus didymus J. E. Smith, were analyzed with technique of polyacrylamide gel electrophoresis.

采用聚丙烯酰胺凝胶电泳，比较了菘蓝属5种2变种1多倍体品种及1外类群种共计20个样本的酯酶同工酶和超氧化物歧化酶同工酶的酶谱差异，并运用数量分类学的原理和方法对酶谱数据进行了聚类分析。

Cuspidate with 33％ identity and 50％ similarity to taxadien-5α-ol-O-acetyltransferase, 34％ identity and 52％ similarity to 10-deacetylbaceatin Ⅲ-10-O-acetyltransferase, and 33％ identity and 49％ similarity to taxane 2α-O-benzoyltransferase, respectively, moreover, it contained the conserved HXXXDG motif that is found in other transacylases, this sequence element has been suggested to function in acyl group transfer from acyl CoA to the substrate alcohol.

No.AF448807）在氨基酸水平与大部分植物来源的酰基转移酶具有较大的同源性，其中与东北红豆杉紫杉二烯-5α-醇-乙酰转移酶的一致性达33％、相似性达50％，与东北红豆杉紫杉10-去乙酰巴卡亭Ⅲ-10-0-乙酰转移酶的一致性达34％、相似性达52％，与东北红豆杉紫杉二烯2α-0-苯甲酰转移酶的一致性达33％、相似性达49％，并且在TS4的氨基酸序列中还发现酰基转移酶家族成员特有保守序列HXXXDG，这个序列单元被认为在将酰基CoA转移至醇类底物的过程中发挥作用。

The main products are: enzymes (phytase, compound enzyme, xylanase, protease, pectinase, amylase, cellulase enzymes) Probiotics (dimethylamino potassium compound probiotics), chemical (sugar beet alkali, etc.) the current domestic market coverage 25 provinces, autonomous regions, the international market has also covered some 20 countries.

主要产品是：酶制剂（植酸酶，复合酶，木聚糖酶，蛋白酶，果胶酶，淀粉酶，纤维素酶等）益生素，化工类目前国内市场覆盖25个省区，国际市场也覆盖20几个国家。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

The oversaturation of cholesterol in bile results in the formation of cholesterol stones. The liver is the organ which synthesizes cholesterol and secretes bile. Liver lipid metabolism research helps to explain the mechanism of cholelithiasis. Utilizing biochemical and biomolecular technology, we have done experimental work as follows: Analyse the ingredient changes of blood and bile samples from patients.

肝脏是合成胆固醇、分泌胆汁酸的器官，对肝脏胆固醇代谢的研究有助于阐明胆石症形成的原因，本文用生物化学和分子生物学的方法，分析了病人血液、胆汁中各种脂类成份的变化，以及肝脏胆固醇合成限速酶3-羟-3-甲戊二酸辅酶A还原酶（HMG-CoA Reductase）、胆汁酸合成限速酶7α-羟化酶（7α-hydrolase）的基因表达。

Lysozymes are classified into six types according to their molecular structures, molecular weights and sources: plant type lysozyme, bacteria type lysozyme, phage lysozyme, chicken egg-white lysozyme (c-type, clyz), goose egg-white lysozyme (g-tpye, glyz) and invertebrate type lysozyme. The most common ones in the nature are c-type, g-type and phage lysozymes.

溶菌酶根据分子结构、分子量和来源的不同可分为六类：植物溶菌酶、微生物溶菌酶、噬菌体溶菌酶、c型、g型和I型3种动物溶菌酶，其中c型、g型和噬菌体溶菌酶最常见。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。