类酶

Antifolate Alimta derived from pyrrolo[2,3-d]pyrimidine is a multitargeted antitumor agent, it can inhibit at least five major folate-dependent enzymes: thymidylate synthase, dihydrofolate reductase, glycinamide ribonucleo -tide formyltransferase, aminoimidazole ribonucleotide formyl transferase and C-1 tetrahydrofolate synthetase(C1-S).

吡咯并[2，3-d]嘧啶类叶酸拮抗剂Alimta是一种多靶向的抗癌药物，它能抑制至少五种叶酸依赖性酶：胸苷合成酶、二氢叶酸还原酶、叶酰多聚谷氨酸合成酶、甘氨酰胺核糖核苷酸转甲酰酶及C1-四氢叶酸合成酶(C1-S)。。

ResultsThe five kinds of extracts extracted by different heteropolarity dissolvents but ligarine extract, the free and conjugated anthraquinones significantly decreased the activities of aminopherase. The processed products of Semen Cassiae, the crude and the parched

总蒽醌类成分是保肝降酶的主要活性成分；除总蒽醌外尚有其他多种保肝降酶的活性成分，有待于对不同提取物作进一步的活性成分追踪研究；决明子的生、炒两类炮制品在用于保肝降酶活性时以生品为佳。

The SOD and the LDH-1 activities and EST (R_f=0.17 and R_f=0.72) at 1℃weresignificantly higher than the values of the room temperature(P＜0.05), but the total enzymeactivities of LDH, G6PDH and EST at room temperature and 1℃were similar(P＞0.05).This showed well-developed anoxia tolerance of the Heilongjiang wood frog to maintainplasma viability when low temperature interrupts the delivery of oxygen and substrates. LDH-1rose significantly avoided the liver lactic acid accumulation that may created the liver to bewearied. EST (R_f=0.17 and R_f=0.72) rose may enhance the ester metabolism to offer muchenergy. These showed steady metabolic rate and relative stable organism environment of theHeilongjiang wood frog to survive under low temperature.Native PAGE separated proteins of liver tissue successfully on the better condition is 6％final gel concentration and there were 15～19 bands identified on this condition.

同功酶电泳图谱显示，1℃组与室温组黑龙江林蛙肝脏LDH酶活性差异不显著，均以LDH-5为主，但1℃组LDH-1显著高于室温组(P＜0.05)，推测黑龙江林蛙为适应低温低氧环境，增强LDH-1酶活性以较好的利用乳酸，避免因大量乳酸形成造成肝脏疲劳；而1℃组肝脏G6PDH酶活性无显著变化，进一步说明低温条件下葡萄糖戊糖途径和糖酵解作用的互相协调，维持机体代谢的稳定；1℃组和室温组EST总酶比活力无显著变化，但1℃组中R_f=0.17和R_f=0.72的EST同工酶显著升高(P＜0.05)，推测在低温条件下黑龙江林蛙可能通过选择性增强部分酯酶同功酶活性，增加肝脏酯类物质代谢，为机体提供维持生命必需的最低能量。

The research status and development trend of biomimetic delignification catalyzed by metal complexes were reviewed.

综述了目前金属配合物仿酶催化脱木质素的研究现状及发展趋势，着重评价了卟啉类和非卟啉类金属配合物作为木质素氧化降解的仿酶催化剂的效果，对金属配合物仿酶催化脱木质素技术的应用前景进行了展望。

The Schiff base transitional metal complexes of various structures and their metallomicelles as peroxidase models for the phenol and phenolic derivant oxidation have been investigated. Some results are obtained from the studies:(1) the Schiff base transitional metal complexes have high catalytic activity for the phenol and phenolic derivant oxidation, and the structure of the complexes has a great effect on its catalytic activity;(2)a novel kinetic mathematical model for the complexes and their metallomicelles catalyzing the phenolic derivant oxidation has been established;(3) the feasibility and mechanism for the phenolic derivant oxidation catalyzed by the metallomicelle(as the mimetic model of peroxidase)have been discussed.

研究了冠醚Schiff碱金属配合物作为过氧化物酶的模拟模型对酚类物质氧化反应的催化作用，取得如下结果：(1)几种冠醚Schiff碱金属配合物对催化酚类物质氧化具有良好的催化活性，其中配体结构对其催化活性有较大的影响；(2)建立了金属配合物与表面活性剂形成的金属胶束催化酚类物质氧化反应的动力学数学模型；(3)探讨了金属胶束模拟过氧化物酶催化酚类氧化反应的可行性及其机理。

Among 90 positive clones in the 16S rDNA library of microorganisms, 23 OTUs were identified based on the similarity of the ARDRA banding profiles. Sequence analysis revealed diverse phyla of bacteria in the 16S rDNA library, which consisted of alpha, beta, delta subclasses of the Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Acidobacteria and Planctomycetes.

采用限制性内切酶HaeIII和RsaI对克隆文库中的90个克隆子进行了酶切分型，根据ARDRA酶切图谱的不同，可将其分为23个OTUs.16S rDNA序列分析结果表明，该克隆文库中主要包括变形菌门的alpha、beta、detla亚类、厚壁菌门、放线菌门、拟杆菌门、酸杆菌门及浮霉菌门等8类细菌及未培养细菌。

Phenylmethylsulfonyl fluoride (174.2 mg/mL), chicken ovomucoid (1000 mg/mL) and soy-bean trypsin inhibitor (1000 mg/mL) could inhibit enzyme activity, which indicated that the enzyme belonged to serine protease group. On plasminogen-free fibrin plates and plasminogen fibrin plates, the fibrinolytic activity had no obvious difference, indicating that the enzyme was a fibrinolytic enzyme which degraded fibrin directly, but not a plasminogen activator which degraded fibrin by activating plasminogen.

此菌株产生的纤溶酶在50℃以下和pH5.0~11.0范围内具有较好的稳定性，最适作用温度为42℃；最适pH值为9.0；Mg2+、Ca2+对此酶有明显的激活作用，而Cu2+能完全抑制酶的活性；174.2 mg/mL的苯甲基磺酰氟、1000 mg/mL的鸡卵类粘蛋白和1000 mg/mL大豆胰蛋白酶抑制剂能完全抑制酶活性，初步说明此酶属于丝氨酸蛋白酶类；体外溶纤作用表明，该酶溶解纤维蛋白的方式是直接溶解，而不是通过激活纤溶酶原。

The identification of protein were performed by searching the mass spectrometry data from the NCBInr database using the software of Sequest Bioworks3. 2. The results indicated that over 25 protein components including acid and basis phospholipase A2, nerve growth factor, presynaptic neurotoxin, hemorrhagic factor, thrombin-like enzyme, serine protease, glycogen synthase, metalloproteinase, maturase, L-amino acid oxidase, plasminogen activator, adhesion, cysteine-rich secretory protein, disintegrin, fibronectin-binding protein, fibrinogen clotting inhibitor A chain, vascular apoptosis-inducing protein 1, pyranose 2-oxidase, carbamoyl-phosphate synthase, as well as the homology proteins of snake venom ablomin, ablomin, catrin, mucrofirase, pallabin, salmosin and triflin homologues were identified from the GSSV.

从GSSV中鉴定出超过25种蛋白组分，主要包括：酸性磷脂酶A1、碱性磷脂酶A2、神经生长因子、突触前神经毒素、出血因子、类凝血酶、丝氨酸蛋白酶、糖原合酶、金属蛋白酶、成熟酶、L-氨基酸氧化酶、纤溶酶源激活物、外源凝集素、富含半胱氨酸分泌酶、去整合素、纤连蛋白结合蛋白、纤维蛋白原凝固抑制因子A、血管凋亡诱导蛋白1、氨甲酰基磷酸合成酶、吡喃糖－2-氧化酶以及ablomin， catrin， mucrofirase， pallabin， salmosin和triflin的同源蛋白。

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制，酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力，酶蛋白亚基间的相互作用，酶蛋白的折叠以及内运动状态与酶活力的关系，酶蛋白发挥功能时的结构特性；运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性，热稳定性机制；确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位；确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构；确定了一个新型的染色质蛋白Cren7的溶液三维结构；正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

The β-CA of B.napus was located in chloroplast stroma and had the conservative motif Cys-X(subscript n-His-X2-Cys peculiar to β type carbonic anhydrases. In the secondary structure,α-helix, random coil,β-sheet, and β-turn were 39.88%, 39.27%, 16.31%, and 4.53%, respectively. The three-dimensional structure was constructed by homology modeling. The 3-D alignment of β-CA from B.napus and a monomer of β-CA octamer from pea showed that the 3-D structure of β-CA from B.napus could match well with the monomer. The alignment results Implied that β-CA from B.napus was a monomer of the β-CA octamer from B.napus.

预测结果显示，甘蓝型油菜β破酸酐酶是定位于叶绿体基质的蛋白质，具有β类碳酸酐酶所特有的保守性基序Cys-χ-His-χ2-Cys；SOPMA预测二级结构显示α螺旋(39.88%)、随机卷曲(39.27%)、β折叠(16.31%)和β转角(4.53%)；用同源建模法构建了三维结构图；通过VAST矢量比对工具将甘蓝型油菜β碳酸酐酶与模板进行三维结构比对，显示甘蓝型油菜β碳酸酐酶与碗豆β碳酸酐酶同型八聚体中的一个单体很好的匹配，推测甘蓝型油菜β碳酸酐酶全酶也是同型八聚体。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。