类粘蛋白

CDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique, and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1335bP的片断编码444个氨基酸残基。

Insect resistance to Bt toxin is related with the variation of cadherin-like protein gene.cDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique,and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1 335 bp的片断编码444个氨基酸残基。

It also can be used as the controlling indicators for membrane bio-fouling in the prophase. When the bio-fouling membrane was anatomized, it shows that the bio-fouling membrane are meshy and coupling, whose primary ingredients are organics matter such as albumin, benzene ring, hydroxyl acid, fatty acid and so on, as well as microorganisms such as cocci, bacilli, leaf mold, alga and so on.

微生物污染解剖试验分析表明，严重的微生物污染薄膜成网状粘联结构，分析到的主要成分有白蛋白、苯环类、脂肪酸和芳香烃类等有机物，可观察到的微生物有球菌、杆菌、霉菌和藻类。

We have identified 1452 GPCRs in the X.tropicalis genome.(2) Phylogenetic analyses classified these receptors into seven families, consisting of Glutamate,Rhodopsin,Adhesion,Frizzled,Secretin,Taste 2 and Vomeronasal 1.(3) X.tropicalis shares a more similar repertoire of GPCRs with mammals than it does with fish.

2严格的系统发生学分析将热带爪蟾G蛋白偶联受体分成了谷氨酸盐受体类，视紫红质类，粘着蛋白类，卷曲蛋白类，味觉2受体类，分泌素受体类和犁鼻骨1受体类这七个受体家族。

As a prime of nourishing soup, the soup combine pig marrow and several kinds of famous herbal medicine such as Chinese caterpillar fungus,decocted for sevral hours, fully dissolve similarity of mucilage glue in soup,milky soup gathered plenty of calcium, phosphorus,iron,amino acid,collagen which is very important to people's helthy,the taste is savory,lubricative,mellow without heavy,having healthy preserving and hairdressing dual effect.

作为滋补汤类的上品，它精选了猪骨髓配以虫草等数十味上等名贵中草药，经过数个时辰的煨制，使骨头的类粘朊等，充分溶解于汤内，奶白色鲜骨汤凝汇了丰富的钙、磷、铁、氨基酸及胶原蛋白等补充人体需要的营养元素，喝之鲜香润滑、醇而不腻，并伴有醇厚粘唇的口味，具有补益养生和美容养颜双重功效。

Phenylmethylsulfonyl fluoride (174.2 mg/mL), chicken ovomucoid (1000 mg/mL) and soy-bean trypsin inhibitor (1000 mg/mL) could inhibit enzyme activity, which indicated that the enzyme belonged to serine protease group. On plasminogen-free fibrin plates and plasminogen fibrin plates, the fibrinolytic activity had no obvious difference, indicating that the enzyme was a fibrinolytic enzyme which degraded fibrin directly, but not a plasminogen activator which degraded fibrin by activating plasminogen.

此菌株产生的纤溶酶在50℃以下和pH5.0~11.0范围内具有较好的稳定性，最适作用温度为42℃；最适pH值为9.0；Mg2+、Ca2+对此酶有明显的激活作用，而Cu2+能完全抑制酶的活性；174.2 mg/mL的苯甲基磺酰氟、1000 mg/mL的鸡卵类粘蛋白和1000 mg/mL大豆胰蛋白酶抑制剂能完全抑制酶活性，初步说明此酶属于丝氨酸蛋白酶类；体外溶纤作用表明，该酶溶解纤维蛋白的方式是直接溶解，而不是通过激活纤溶酶原。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失