类特异性的

The technique path of the research is : on the rules of obtainable of data, specialty, sensitivity, representative, characteristic of full-scale and perspicuity of indexes, used external statistic way which includes ways of a coefficient of variation, case cluster and relation analysis, variable cluster and subjective Delphi, sift from initial index system, and finally get a complete evaluation index system for labor relation which meet the external technique requirement and subjective theoretic and practical analysis.

本论文研究的技术路径为：以指标资料的可获得性，指标的特异性、敏感性、代表性、全面性和简明性为原则，运用包括变异系数法、样本聚类和相关分析法、指标聚类法等客观的数理统计方法和主观的德尔菲法，对初始的指标体系进行了筛选，最终确定了满足客观技术要求和主观理论经验的完整的劳动关系评价指标体系。

Compared with classical cytotoxic T lymphocytes , NK cells are superior in the following aspects: NK cells are among the first cell types to be activated after intracellular pathogen stimuli, the activity of NK cells peaked between 1 to 3 days, retained during the first 7-10 days, and the T cells response emerged only after 7 days when the NK cell responses begin to decline; though comprising of only 10％-15％ of peripheral lymphocytes, NK cells mobilize most of the cell populations in a strong and high level response to different invasions, and act as key factors at early defense before a specific immune response could mount, while only certain clones were involved in T cell response; NK cells kill virus-infected or malignant transformed cells without pre-sensitization and without restriction by major histocompatibility antigen, which is usually a mechanism of immune escape to T cell recognition by virus-infected or tumor cells, thus NK cell in complementary with T cells play crucial roles in tumor and virus eradication.

NK细胞以其强大的细胞毒活性为特征，与细胞毒性T细胞相比，NK细胞具有如下特点：NK细胞虽然只占外周血淋巴细胞的10-15％，但是对刺激性因素产生应答的过程十分迅速，一次可以调动大多数细胞共同参与，而不是几个克隆的活化，免疫应答的强度高；病毒感染或恶性转化细胞的一个主要特征是细胞表达的MHCⅠ类分子下降或消失，并借助这一机制逃避特异性T细胞应答的识别，NK细胞介导的杀伤活性无需抗原刺激，不受MHCⅠ分子表达的限制，从而与T细胞应答互为补充发挥免疫防御的功能；NK细胞的免疫活性可以被多种细胞因子上调，其中IFNα，IL-2，IL-12，IL-15和IL-18具备更强的作用；NK细胞具有强大的细胞因子分泌功能，对于启动T细胞特异性应答必不可少。

Objective: Tryptase is specific for mast cell.

目的：类胰蛋白酶是人肥大细胞特异性酶，研究表明类胰蛋白酶在急性粒细胞白血病中也有不同程度的表达。

Physostigmine, a nonspecific centrally acting arousal agent, appears to antagonize the central nervous system depressant effects of both volatile anesthetic agents and benzodiazepines with some success.

毒扁豆碱，一种非特异性中枢唤醒药，有拮抗挥发性麻醉药和苯二氮卓类药中枢抑制效应成功的例子。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

We selected mecA gene and femA factor asβ-lactam antibiotic-resistant genes, ermA, ermC, msrAgene as macrolides-lincosamids antibiotic-resistant genes, norA, grlA, gyrA, gene as Quinolonesantibiotic-resistant genes, and tetM gene as Tetracycline antibiotic-resistant genes on the basis of the latestresearch. We designed primers with GenBank enunciable gene sequence, cloned the drug resistantgenes, and sequenced these genes.

根据国内外最新文献报道，筛选出对β-内酰胺类抗生素耐药的mecA基因及辅助因子femA；对大环内酯类及林可霉素类抗生素耐药的ermA、ermC、msrA基因，对喹诺酮类抗菌药物耐药的norA、grlA和gyrA基因及对四环素类抗生素耐药的tetM基因作为拟选的主要耐药基因，分别设计PCR扩增的特异性引物，进行克隆。

Laccases are able to oxidise both phenolic and nonphenolic compounds. They have broad ranges of subsrate specificity. Therefore, they are very useful for applications on pulp bleaching, textile dye decolouration, effluent detoxification, bioremediation and biosensors.

由于漆酶能够氧化芳香类化合物和其它一些非芳香类有机物，具有广泛的底物特异性，因此在纸浆漂白、纺织品染料脱色、有毒废弃物的去除、生物修复和生物传感器等方面具有巨大的应用潜力。

We suppose that there is a presently undefined subfraction of stem cell population that exists in a number of tissues beyond embryonic development. We term them postembryonic pluripotent stem cells. They differ from embryonic stem cells in that they gradually lose some differentiation potentials during development and adopt some special phenotypes once within a certain tissue. They can give rise to different kinds of stem cells; therefore, help to maintain balance during development and metabolism. They provide cells not only for the damaged tissues they reside, but also for damaged tissues different from them by migration triggered by proinflammatory cytokines or growth factors.

我们认为在胚胎发育后的多种组织中都存在一类原始干细胞群体，它们和造血干细胞、神经干细胞等多能干细胞不同，后者只能分化为特定胚层的细胞，而这类原始干细胞可分化为不同胚层的组织细胞；另一方面，它们区别于胚胎干细胞，在妊娠期间会逐渐失去部分分化潜能，并且会出现一些特殊表型或者分子标志，我们称其为胚胎后亚全能干细胞，这类干细胞具有下列特性：可分化为不同胚层的细胞，分布于身体的所有组织和器官，具有相同的表型或标志；他们在胚胎期存在于一些或全部的组织和器官中，可分化为不同类型的多能干细胞并在生长发育和新陈代谢中维持这些多能干细胞保持平衡；可以参与机体自我修复和更新，不仅能分化为所在器官的组织特异性细胞，参与器官重塑，也可以在炎症因子或者生长因子的趋化作用下，远处转移修复受损组织。

With the assistance of the chaperons such as calnexin、calreticulin and tapasin,the molecular heavy chain andβ2 micro globulin of the major histocompatibility complexare assembled together to form the steady complex which is further transported to the surface of the APC through the Golgi body.

在calnexin、calreticulin、tapasin等分子伴侣的协助下，内质网腔内的主要相容性复合体(major histocompatibilitycomplex，MHC)Ⅰ类分子重链、β2微球蛋白以及相应多肽片段聚集成较为稳定的复合物，经高尔基体被进一步转运到APC表面；MHCⅠ类分子与抗原肽的有效结合是特异性细胞免疫应答的重要的环节。

The 59 pure cultural isolates, including 3 Fusarium oxysporum f.sp.cucumarinum, 26 other Fusarium isolates and 20 fungi, 6 bacteria, 7 actinomycetes from cucumber rhizosphere, have been detected by PCR-RFLP and Nested-PCR. It shows that PCR-RFLP is special for the pathogen of Cucurbits Fusarium Wilt, and Nested-PCR is special for Fusarium oxysporum f.sp.cucumarinum.

本论文应用PCR-RFLP和巢式PCR程序对3株黄瓜尖镰孢菌(Fusarium oxysporum f.sp.cucumarinum)、26株镰孢属部分真菌及分离自土壤的20株真菌、6株细菌和7株放线菌共59个菌株进行扩增，表明PCR-RFLP程序对瓜类枯萎病菌具有特异性，而巢式PCR对黄瓜尖镰孢菌具有特异性，巢式PCR程序检测黄瓜尖镰孢菌的特异性高于PCR-RFLP程序。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。