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Embryonic germ cell lines were isolated and cloned from gonad,gonadal ridge,analogue or the surrounding tissues of 9.5~13.5 days postcoitum mouse fetuses.Primordial germ cells were co-cultured with mouse embryonic fibroblasts obtained from the same conceptus.

自交配后9.5~13.5 d小鼠胎儿生殖嵴/腺或其类似物及周围组织,采用与其同源胎儿成纤维细胞共培养的方式分离克隆到具小鼠ES细胞诸多特征的胚胎生殖细胞系。

The sequence specific assay established in the present study can be used for accurate quantitation of GH mRNA in cyprinid species, and contribute to a better understanding of the regulation of GH mRNA expression in teleosts.

这表明:性类固醇激素不能在转录水平上对生长激素基因表达进行调控,但能够加强促性腺激素释放激素类似物在刺激生长激素分泌及调控其基因转录水平方面的作用。

Octreotide can also be synthesized by solid-phase method, and its molecular weight is 1019.3 dalton.

奥曲肽也可以通过固相合成的方法获得,是生长抑制激素抑制素的八肽类似物

As to the plenty of include bodies in the precipitation , denationalization, detergence, purification and dissolution, last regeneration were recommended to acquire great deal of expressed GST fusion proteins.

COlt BLZI生产融合蛋白,重组后的 DNA序列包括一个PGEX质粒,依照PGEX质粒的融合蛋白的表达是在tao启动子的控制之下,而枯启动子可由乳糖的类似物I刚来诱导它表达。

Objective To establish a method of constructing a living dermis equivalent in vitro.

目的:探寻建立真皮类似物的培养方法。

The venom of elapid snakes contains a number of small proteins that display a broad spectrum of pharmacological activities.In study on the neurotoxin from the venom gland of Bungarus multicinctus,five cDNA sequences (MNCTL1-a,MNCTL1-b,MNCTL1-c,MNCTL1-d and MNCTL2) encoding two novel proteins were obtained from the total RNA by reverse transcription-polymerase chain reaction.

采用SMART技术和RT-PCR方法,从同一个银环蛇毒腺中克隆到了5 种尚未报道过的心脏毒素类似物全长cDNA序列(MNCTL1-a、MNCTL1-b、MNCTL1-c、MNCTL1-d和MNCTL2)。5种序列中,MNCTL1-b、MNCTL1-c、MNCTL1-d和MNCTL2的长度均为505 bp,包括5′非翻译区32 bp,3′非翻译区212 bp和其余261 bp组成的一个完整开放阅读框;MNCTL1-a 由于3′非翻译区24 bp的缺失,全长cDNA仅为481 bp。

The separate factor between S--α-phenyl ethylamine and acetophenone for all polymers was above one,and the maximum ofαwas 2.86.This indicated that all polymers had good selection.

各种印迹聚合物对于S--α-苯乙胺及其结构类似物苯乙酮的分离因子均大于1,最大达到2.86,显示出了很好的选择性。

AIM: To obtain a high and stable expression analog of human basic fibroblast growth factor by genetic engineering.

目的:用基因工程的方法获得高效稳定表达的重组人碱性成纤维细胞生长因子结构类似物

In this study, curcumin and 23 novel analogs were used to screen for inhibition of matrix metalloproteinase-9 (MMP-9) activities, protein and gene expression of highly metastatic HT-1080 human fibrosarcoma cells to explore the mechanisms of action.

本篇研究使用姜黄素及其二十三个结构类似物对於具有高度转移活性的人类纤维肉瘤细胞(HT-1080)进行MMP-9活性、蛋白质及基因表现抑制活性筛选,并探讨其可能机制。

Becausethe ginger flavine and its the synonym have many kinds of pharmacologyactiveness, at present, conducted the research take this kind ofcompound as the forerunner which the structure optimized to cause inthe world scope chemist and the medicine scientist's widespreadattention, take the ginger flavine as the raw material, first had thefunction production corresponding sodium salt in the organic solventby NaOH or NaOEt and a-H, then and 卤代烃carried on the substitution reaction, the response condition which theresearch optimized, and grew this derivative monocrystal with thesolution law.

由于姜黄素及其类似物所具有的多种药理活性,目前,以该类化合物为先导进行结构优化的研究引起了世界范围内的化学家和药物学家的广泛关注,以姜黄素为原料,先在有机溶剂中以NaOH或NaOEt与a-H发生作用生成相应钠盐,再和卤代烃进行取代反应,研究优化的反应条件,并用溶液法生长该衍生物单晶体。

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