管细胞

DMBQ, ferulic acid and resorcin were effective chemical signal substances in the process of seed germination and haustorium formation of Cistanche deserticola. 4.The embryo is consist of 12 cells, the endosperm cell degraded so as to provide nutrition for the embryo development .As a result, germ tube differentiated from the embryo and came out through micropylar end, the small cell at the tip of the germ tube held the capacity of cleavage and the other cells grew big ,so the germ tube elongated .The anterior extremity of germ tube swelled to form haustorium under chemical substances treatment, the haustorium cell stained ,especially its portrait because of the lignification of the cells.

肉苁蓉种子的胚是由12个细胞紧密排列形成的圆球状胚，球形原胚分裂增殖的同时，胚乳细胞分解，为胚细胞的增殖提供营养，最终胚上分化出的芽管由珠孔端冒出，芽管前端细胞较小，具有旺盛的分裂能力，后部细胞体积增大，芽管逐渐伸长，在化学物质的诱导下，芽管由中部至前端细胞膨大分化出初生吸器，初生吸器呈帽子状，而这些细胞比其周围的细胞着色深，吸器纵向局部区域的番红染色加深，细胞高度木质化。

Results: TFF2 peptide was distributed in cytoplasm of epithelial cells of the intercalated duct, striated duct and interlobular duct, and positive reaction was found in the cavity, especially near the cavosurface of epithelial cells in the striated duct and interlobular ducts.

结果：TFF2免疫反应阳性物质主要位于闰管、纹状管和小叶间导管上皮细胞胞质，特别是近管腔面更多，纹状管和小叶间导管管腔内亦有阳性物质分布；颗粒区管的少颗粒暗细胞或无颗粒细胞强阳性；多颗粒细胞弱阳性或阴性。

Results: the positive cells mostly included ependymal cells of myelocoele, glial cells, anterior horn motor neurons, and epithelial cells of spinal pia mater and their expressions were mainly located on cell membrane. interestingly, aqp4 distribution in glial cells and ependymal cells showed certain directivity, but the former was mainly in the membrane side contacting with capillary endothelial cells and the latter mostly in the basolateral membrane of ependymal cells.

结果： 免疫组织化学染色和原位杂交结果显示胶质细胞、前角运动神经元、中央管室管膜细胞、软脊膜上皮细胞均表达阳性，aqp4主要分布于细胞膜，其中，胶质细胞和中央管室管膜细胞表达存在极性，前者主要分布在与毛细血管内皮细胞接触的一侧，后者主要分布在中央管室管膜细胞的基底侧膜。

During the fertilization of angiosperms, the degenerated synergid induces a pollen tube to enter and break the pollen tube to release two sperm cells, which fuse with the egg and central cell.

在受精过程中，助细胞吸引花粉管向雌配子体生长，并接受花粉管长入细胞程序死亡助细胞中。接下来的花粉管停止生长和花粉管顶端破裂释放出2个精细胞的过程可能也依赖于助细胞。

Theurinary IL-6 level positively correlated with density of glomerular matrix membrane, global sclerosis, fiber or fibrocellular crescents and interstitial fibrosis (p. 05). According to the degree of density of glomerular matrix membrane and interstitial fibrosis, urinary Col-IV level had better correlation than urinary TGF-betal and IL-6 levels.In IgAN, Col-IV showed increased expression in diseased renal tissue whereas the site of expression of TGF-betal was mainly localized within the cytoplasm of tubular epithelial cells. Interstial expressionwas also present but glomerular TGF-betal expression was found only in patients with heavy mesangial proliferation. There was a significant correlation between glomerular positivity for Col-IV and severity of histological damage. There was also a significant correlation between positivity for TGF-betal and Col-IV in the tubular epithelial and interstitial lesions. In contrast, there was no ralationship between glomerular positivity for TGF-betal and severity of histological damage.The urinary TGF-betal level paralleled tubular TGF-betal expression.

结果 ①IgAN患者尿TGF-β1、IL-6、Col-Ⅳ水平较健康人明显增高(P＜0.01)，该变化与血中的浓度无关(P＞0.05)；②尿TGF-β1水平与小管间质TGF-β1阳性表达呈正相关(P=0.000)，而与小球TGF-β1阳性表达无关(P＞0.05)，尿Col-Ⅳ水平与小球和小管间质Col-Ⅳ阳性表达均呈良好的相关性(P＜0.01)，还与小管间质TGF-β1阳性表达呈正相关(P＜0.05)：③小球Col-Ⅳ阳性表达与肾组织慢性病变密切相关(P＜0.05)，小管间质Col-Ⅳ和TGF-β1阳性表达均与肾小管间质病变呈良好的相关性(P＜0.01)，而小球TGF-β1阳性表达与肾组织损伤无关(P＞0.05)；④尿TGF-β1、Col-Ⅳ水平与肾小球基质基底膜面密度、小管间质病变呈正相关(P＜0.01)，与小球内细胞数呈负相关(P＜0.05)，该结果与其在组织中的表达一致；尿IL-6水平浙江大学硕士学位论文尿TGF一B一、IL一6和Col一IV在IgA肾病中的应用价值与基质基底膜面密度、球性硬化、纤维或细胞纤维新月体所占肾小球百分数及小管间质病变均有显著的相关性(F.05)；在轻度肾病理损伤时，尿'l'G卜pl、I卜6、Col一IV水平即升高，而尿Col一W在反映细胞外基质积聚和间质纤维化程度上比尿TGF一pl和IL一6有更好的相关性。

Results The expression of the Mahoganoid protein and its mRNA was showed by brown staining and observed in the Leydig cells, spermatogonia, primary spermatocytes, spermatids, Sertoli cells, and peritubular myoid cells of testicular tissues and principal cells, basic cells and epithelial cells of epididymal tissues.

结果1。免疫组化法检测Mahoganoid蛋白的实验结果显示的棕色阳性信号可见于各天龄段大鼠睾丸的生精细胞、支持细胞、间质细胞和管周肌样细胞以及附睾输出管的高柱状、低柱状上皮细胞和附睾管的上皮细胞、主细胞、基细胞。其中Mahoganoid蛋白主要表达于胞膜和胞浆。

Results: The results showed that:(1) The three types of NOS immunoreactivity were not found in rat testis until 30 days after birth;(2) NOS1 immunoreactivity was found in a few spermatocytes in rat testes on postnatal 30 days, and in spermatozoa present at the lumen surface of seminiferous tubules and some Leydig cells on postnatal 60 days;(3) NOS2 immunoreactivity appeared in a few spermatocytes, Sertoli cells and peritubular myoid cells on postnatal 30 days. On postnatal 60 days, NOS2 immunoreactivity appeared in some Leydig cells, peritubular myoid cells, Sertoli cells, very few spermatocytes and the head of immature spermatozoa in some seminiferous tubules;(4) NOS3 immunoreactivity was detected in a few spermatocytes on postnatal 30 days, as well as in the smooth muscle cells of blood vessels on postnatal 30 days and 60 days.

结果：(1)生后4、7、14 d大鼠睾丸未见3种NOS免疫阳性反应；(2)生后30 d少数精母细胞及生后60 d生精小管腔面精子和间质细胞呈NOS1阳性；(3)生后30 d少数精母细胞、支持细胞和管周类肌细胞呈NOS2阳性，而生后60 d NOS2阳性反应见于睾丸间质细胞、管周类肌细胞、支持细胞、极少数精母细胞和不成熟精子头部；(4)生后30 d睾丸内少数精母细胞和血管壁呈NOS3阳性，生后60 d NOS3阳性反应仅见于血管壁。

The testis index, testis volumes were same as the annual changes of testis mass. The curves of annual variation were all unimodality.2 The spermatogenetic cycle of Myospalax cansus comprises seven stages with significant features: Stage I , from February to April, the testis were at the stage of spermatogonia proliferation. In this period, testis index and the number of spermatogonia began to rise. Other spermatogenic cells had not yet formed; Stage II to III, from March to April, primary spermatocyte meiosis period. The testis index was highest in this stage, and spermatogenic cells were in spermatocyte stage, the primary spermatocyte meiosis generated to secondary spermatocyte; Stage IV, from April to May, spermatocytes continued to split, germ cells appeared in seminiferous tubules; Stage V, in May, sperm formation, spermatids of seminiferous tubules were transformed to spermatozoa, a large number of sperms existed in the lumen; Stage VI, spermatozoa emission period, from May to June, testis index were a significant drop and mature spermatozoa excluded gradually; VII, the testicular activity ceased basically from July to September, November to January of the following year, the spermatogenic activity ceased completely. Therefore, Myospalax cansus are animals of seasonal reproduction, spermatogenesis cycle is discontinuous type.

睾丸系数、体积和重量的年周期变化规律一致，变化曲线呈现单峰型。2甘肃鼢鼠雄性生殖腺的年周期活动由7个特征明显的时期构成：Ⅰ期，2～3月份，精原细胞增殖期，睾丸系数开始上升，精原细胞进行有丝分裂，其他生精细胞尚未形成；3～4月份为Ⅱ～Ⅲ期，初级精母细胞成熟分裂期，睾丸系数达到最大，生精细胞大多处于精母细胞阶段，初级精母细胞减数分裂生成次级精母细胞；Ⅳ期，4～5月份精母细胞继续进行分裂，精细胞在生精小管内出现；Ⅴ期，5月份，精子形成期，曲细精管中精细胞变态成精子，在管腔中存在大量的精子；Ⅵ期，精子排放期，5～6月份，睾丸系数显著下降，成熟精子从生精小管上脱离，逐渐排除；Ⅶ期，精原细胞停滞期，7～9月份睾丸生精活动基本停滞，11～翌年1月，生精活动完全停止。

The ciliates were always ideal materials in the studies of the structure and function of cytoskeleton and its cell control and adjustment, for they have many kinds of cytoskeletons with complexity and they could be easily cultured and handled.

由于原生动物纤毛虫具有多种复杂的细胞骨架结构，加之其材料培养和处理的优点，一直被作为研究细胞骨架结构与功能及其细胞调控的理想材料。20世纪80年代初，许多学者应用蛋白银染色方法和超微结构方法，显示了纤毛虫细胞皮层中以纤毛基体作为主成分的骨架结构，探讨了纤毛虫皮层纤毛器的形态、形态发生及其结构形成机理，在国内外原生动物细胞学领域成为主流。20世纪90年代起，先后应用非离子去垢剂处理和扫描电镜相结合的方法、稳定保存微管结构的透射电镜术，微管蛋白的抗体标记及其免疫荧光技术及相关的生化与分子生物学等方法显示纤毛虫的微管胞器及其微管蛋白组分，探索微管胞器的形态、功能及其微管装配机理，积累了较多的资料，取得了重要的进展。

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy（32/120; 26.7%）. The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders（22/120; 18.3%）. Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found（12/120; 10%）. Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters（8.3%）. Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters（4.2%）had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现，在总共搜集的120个病例中，其中因年老所导致的产精力不佳为最多，占38例（31.7%），於镜下可见大量精虫黏附於Sertoli cell的表面，并可见Sertoli cell数量明显增多而Leydig cell明显减少，且其细胞核呈现浓染的现象，而在其副睪中仍可见到成熟精虫蓄留於管腔中，但在其输精管内却只有少量精虫存在；其次为睪丸萎缩，占32例（26.7%），镜下可见中度至重度无造精作用，其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在，但Leydig cell数量明显减少且其细胞质内的脂质也明显减少；睪丸发育不全，占22例（18.3%），於生精小管内只见到精母细胞及少量Sertoli cell存在，不见造精细胞分化，於生精小管间质可见Leydig cell减少并伴随结缔组织增生；副睪结石，占12例（10%），肉眼下可在副睪及输精管前段见到黄白色大小不一的结节，触感坚硬，於镜下可见副睪管扩张并有大量成熟精虫蓄积，严重时可见钙化现象，以Von Kossa及茜素红染色均呈阳性反应；类淀粉沉著症，占10例（8.3%），镜下在睪丸间质及血管周围可见粉红均质样的物质沉积，以刚果红染色成阳性反应；马立克病，占5例（4.2%），镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润；睪丸坏死，占1例（0.8%），镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。