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In chapter 5, the concept of zero-span tension known in papermaking area is for the first time introduce to wood science area to explore in-tree variation of longitudinal tensile strength of tracheids and its main influencing factors. In chapter 6, composite micromechanics and classic laminated theory are used to make a comprehensive and detailed analysis of the main influencing factors of mechanical properties of tracheids cell wall. Some experimental results acquired in the foregoing chapters are explained successfully. Results were summarized as follows: Mechanical characteristics of wood at micron scale: 1 At micron scale, the longitudinal mechanicai behavior of wood microtome section differs greatly from that of wood with normal size.

论文的第2章首先研究了微米尺度下木材的力学特性,为后面运用木材微切片拉伸技术奠定一定的理论基础;在第3章,首次考虑到了微米尺度下木材力学性质的尺寸效应,并运用木材微切片正常间距拉伸技术研究了管胞纵向弹性模量的株内变异规律;在第4章,首次利用纳米压痕技术中最新发展起来的连续刚度测量法直接在管胞细胞壁上进行纳米级的压痕实验,测量次生壁S〓层的纵横向弹性模量和硬度,从而把细胞壁力学的研究提高到一个更高的水平;在第5章,首次把造纸领域的零距拉伸技术引入木材科学研究领域,研究了管胞纵向抗拉强度的株内变异规律及其主要影响因素;第6章则运用复合材料细观力学的基本理论和经典层板理论对影响细胞壁力学性能的主要因素进行了全面而系统的分析,并为前几章的一些实验结果提供理论上的解释。

Results The anesthesia range was narrowed and anesthesia duration was shortened with the prolongation of epidural catheter placement,Epidural roentgenography showed that the medium diffused normally within epidural space,biopsy of epidural tissues showed that there were inflammative cells infiltration and fiber proliferation with longer epidural catheter placement(more than 4 weeks),without significant differences between both groups.

结果 随着硬膜外腔置管时间延长,麻醉阻滞范围变窄,麻醉阻滞时间也明显缩短,两组之间没有明显区别;硬膜外腔造影显示:造影剂在硬膜外腔扩散良好,无明显受阻;组织学检查显示:置管时间长(4周以上)的动物,硬膜外组织炎细胞浸润、纤维组织增生、硬膜增厚,而且随置管时间延长,变化加显著,但两组之间没有明显区别。

Results The anesthesia range was narrowed and anesthesia duration was shortened with the prolongation of epidural catheter placement,Epidural roentgenography showed that the medium diffused normally within epidural space,biopsy of epidural tissues showed that there were inflammative cells infiltration and fiber proliferation with longer epidural catheter placement(more than 4 weeks),without significant differences between both groups.Conclusion Epidural corticosteroids can not prevent epidural adhesion from occurrance induced with long-term epidural catheter placement.

结果 随着硬膜外腔置管时间延长,麻醉阻滞范围变窄,麻醉阻滞时间也明显缩短,两组之间没有明显区别;硬膜外腔造影显示:造影剂在硬膜外腔扩散良好,无明显受阻;组织学检查显示:置管时间长(4周以上)的动物,硬膜外组织炎细胞浸润、纤维组织增生、硬膜增厚,而且随置管时间延长,这"种变化更"加显著,但两组之间没有明显区别。

The staining of F-actin in the ischemia reperfusion group was decreased significantly (P.05) compared with the normal control group. There was a marked loss of canalicular microvilli and distension of canalicular lumen in the ischemia reperfusion group. The staining of F-actin in the LA group was significantly increased than those in the I/R group (P.05). Conclusions: Ischemia reperfusion induces an disruption of bile canalicular F-actin microfilaments and a loss of microvilli. Alpha-lipoic acid can protect bile canalicular F-actin microfilaments against IRI.

肝脏I/R可造成胆小管F-actin微丝破坏、微绒毛丧失,导致胆小管收缩减弱,胆汁排泄功能受损,这可能是大鼠肝脏I/ R后缺血型胆道病变发生的主要机制;α-硫辛酸通过清除氧自由基,降低脂质过氧化反应,调节细胞内氧化剂-抗氧化剂的含量,保护胆小管F-actin微丝结构免遭缺血再灌注损伤破坏,进而预防和治疗缺血型胆道病变。

Results The anesthesia range was narrowed and anesthesia duration was shortened with the prolongation of epidural catheter placement,Epidural roentgenography showed that the medium diffused normally within epidural space,biopsy of epidural tissues showed that there were inflammative cells infiltration and fiber proliferation with longer epidural catheter placement(more than 4 weeks),without significant differences between both groups.Conclusion Epidural corticosteroids can not prevent epidural adhesion from occurrance induced with long-term epidural catheter placement.

结果 随着硬膜外腔置管时间延长,麻醉阻滞范围变窄,麻醉阻滞时间也明显缩短,两组之间没有明显区别;硬膜外腔造影显示:造影剂在硬膜外腔扩散良好,无明显受阻;组织学检查显示:置管时间长(4周以上)的动物,硬膜外组织炎细胞浸润、纤维组织增生、硬膜增厚,而且随置管时间延长,这&种变化更&加显著,但两组之间没有明显区别。

Before ovulation, the oviducal wall is the thickest, the simple tubular glands in lamina propria are filled with the jelly substance. After ovalation, the oviducal wall is the thinnest, the cells of the tubular glands becomes smaller. During ovulation, the lamina propria is thicker. Before ovulation and after ovalation, the change of serosa is not distinct.

产卵前,输卵管管壁最厚,固有层中的单管状腺充满胶质;产卵后输卵管管壁变薄,固有层腺体细胞缩小,粘膜层在产卵时比产卵前明显增厚;产卵前和产卵后浆膜层无明显变化,产卵时厚度明显变薄。

The cell freezen amount is about 3×106 cell/ml, sample contents of each breed were between 40 and 50, cryopreservation ampules were 240-360 each breed.

每个品种的冻存样本含量为40-50之间,每个品种的冻存管数在240-300支之间,每个个体的冻存管数为6支,每支冻存管的细胞数量为3-5×106个/ml。

JA is a crucial signal molecule in inducing cambia to differentiate the secondary laticifer, and a local high level of JA in cooperating with other plant hormones, especially cytokinin, is prerequisite for the secondary laticifer differentiation.

研究表明,茉莉酸是诱导乳管分化的关键信号分子,局部高水平的茉莉酸是诱导维管形成层细胞分化次生乳管所必需的,同时需要其它激素的协同作用。

In prophase, it localized on the microtubule organizing-center. In metaphase, it co-localized with α-tublin completely on spindle. In anaphase, it co-localized with α-tublin in aster and mid-zone. In telophase, it co-localized with α-tublin in cytoplasmic bridge connecting the two daughter cells.

在分裂前期,hSMP-1蛋白位于微管组织中心区域;在分裂中期,hSMP-1蛋白与组成纺锤体的微管呈完全共定位;在分裂后期,hSMP-1蛋白与星体和纺锤体中区的微管呈共定位关系;在分裂末期,hSMP-1蛋白则与两个子细胞之间的胞质桥完全共定位。

Lamblia were cultivated axenically with modified TYI-S-33 medium contained dihydroartemisinin (0.002 mg/L) for 12 h and then stained by rhodanmine phalloidin and paclitaxel (P22310). The cytoskeletons of the organisms were detected and analyzed by flow cytometry, and then observed by confocal microscopy.RESULTS: The cytoskeleton of trophozoites was markedly damaged after treatment with 0.002 mg/L dihydroartemisinin for 12 h.

将用含双氢青蒿素的改良TYI-S-33培养基培养后的蓝氏贾第鞭毛虫滋养体,用专一性结合f-肌动蛋白的荧光染料罗丹明-鬼笔环肽(rhodanmine phalloidin, RDP)标记微丝,结合微管的紫杉醇(Paclitaxel, Oregen Green 488, P22310)标记微管,用流式细胞术检测、分析,用激光共聚焦显微镜(confocal microscopy, CFM)观察滋养体微丝和微管的变化。

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