简便

The results showed that RSAP used two primers of 18 nucleotides in which starting at the 3' end of each primer were restriction site sequences (4-6 bases),followed 12-14 bases of arbitary sequence, the differences between two primers were restriction sites and filler sequence; PCR am-plification was run for the first 5 cycles with an annealing temperature of 35℃, followed by 35 cycles with an annealing temperature of 48℃; the PCR reaction of 25 μL included 20ng DNA templates, 2.5mmol/L of Mg(superscript 2+), 0.2 mmol/L of dNTP, 1.5U of Taq DNA polymerase, 600nmol/L of each primers. RSAP is a new maker technique with simplicity, moderate throughput ratio and reliability. RSAP is of good reprodicibity and broad application.

结果显示，RSAP技术的引物为2条长度均为18bp的引物，引物的3'端为4~6个碱基的限制性酶切位点序列，接着是12~14个碱基的随机序列，2条引物的限制性位点和随机序列不同：PCR扩增的前5个循环采用35℃的退火温度，随后的35个循环采用48℃的退火温度；在25μL反应体系中，模板DNA用量为20ng，Mg(上标 2+)浓度为2.5mmol/L dNIPs浓度为0.2 mmol/L，Tap DNA聚合酶用碳为1.5U，2条引物浓度均为600mmol/L；RSAP技术重复性好，适用性广泛，是一种操作简便、产率中等、稳定可靠的DNA标记技术。

A simple method for detection of HBV DNA in sera is described in this thesis.

本文介绍了一种简便的检测血清HBVDNA 的方法。

It uses advanced image processing technology, and realizes a series of functions with the simple menu.

该系统应用了先进的图像处理技术，用简便的菜单来实现一系列图像和文件的处理功能。

The TB protein chip system is simple ,rapid,sensitive and specific,and suitable for detection of rapid serodiagnosis of tuberculosis.

芯片系统检测的敏感性和特异性符合临床检验要求，具有简便、快速和单人份操作的优点，可用于临床辅助诊断结核病人。

This system fused the database, Internet, the documents management and so on the many kinds of elements, the operation has been extremely simple, is material management servosystem which set of functions consummated.

该系统融合了数据库、互联网、文档管理等多种元素，操作非常简便，是一套功能完善的资料管理辅助系统。

It is of significance to prevent sex-linked inheritant diseases.

采用孕妇血浆胎儿DNA和巢式PCR技术可以快速简便的进行无创性产前性别诊断，诊断结果的准确率为91.8%，对性连锁遗传病的预防具有重要意义。

A new method of shakedown analysis based on Melan s theorem has been presented in this paper.

本文提出一种以Metan定理为基础的简便的安定性计算方法。

The RAPD is more suitable in the diversity study of potato cultivars, and suitable to analyse the sibship of potato cultivars in breeding.

研究表明：RAPD标记简便、快速、成本低，适用于分析马铃薯遗传多样性，指导马铃薯育种实践中的亲本组配。

The methods of identification and content determination are simp...

结论该鉴别及含量测定方法简便可靠，重复性好，可用于控制产后逐瘀胶囊的质量。

This method was simp le,high sensitive and specific for detecting interleukin 8 in human and rabbit s erum as well as supernatant of cell culture.

该方法是一种简便、灵敏和特异的IL-8分析方法，可用于人和兔血清以及细胞培养上清液中IL-8分析。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。