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With the competent germinating epicotyl of cotton as receptor in exogenous gene transformation, NPTII (neomycin phosphotransferase Ⅱ) gene carrying selection marker and GFP reporter gene were mediated into cotton by agrobacterium-mediated transformation. The pre-culture time, bacterial-immersing time, co-culture time and antibiotics concn., etc of using agrobacterium-mediated transformation in the transformation of competent germinating epicotyl of cotton were studied.

方法]以棉花感受态萌动的上胚轴作为外源基因转化的受体,用农杆菌介导法将携带有筛选标记NPTII基因及GFP报告基因导入棉花,研究了农杆菌介导法用于棉花感受态萌动上胚轴转化的预培养时间,浸菌时间,共培养时间及抗生素处理浓度等。

MethodWiththe competent germinating epicotyl of cotton as receptor in exogenous gene transformation,NPTIIgene carrying selection marker and GFPreporter gene were mediated into cotton by agrobacterium-mediated transformation.The pre-culture time,bacterial-i mmersing ti me,co-culture ti me and antibiotics concn.,etc of using agrobacterium-mediated transformationinthe transformation of competent germinating epicotyl of cotton were studied.

方法以棉花感受态萌动的上胚轴作为外源基因转化的受体,用农杆菌介导法将携带有筛选标记NPTII基因及GFP报告基因导入棉花,研究了农杆菌介导法用于棉花感受态萌动上胚轴转化的预培养时间,浸菌时间,共培养时间及抗生素处理浓度等。

As a consequence of the results of the aforementioned analyses, patients referring pain located at the right epigastrium were selected and further evaluated.

基於前述分析之结果,病患称右上腹部疼痛的,我们会加以筛选,再进一步评估。

The bioactive screening on aldose reductase, nitric oxide synthetase, liver cancer Bel-7402, cavum oris epithelioma KB and colon cancer HCT-8 showed that paeoniflorin inhibited the nitric oxide synthetase by the rate of 83. 4% at the concentration of 5μg/ml, and paeoniflorigenone inhibited the colon cancer HCT-8 cells by the rate of 83. 2% at the concentration of 5μg/ml.

通过醛糖还原酶、NO合酶、肝癌Bel-7402、口腔上皮癌KB以及结肠癌HCT-8五种模型的生物活性筛选表明,在51μg/ml的浓度下,芍药甙对NO合酶的抑制率达83.4%,芍药甙元对结肠癌HCT-8细胞株的抑制率达83.2%,有较好的抑制作用。

The protein encoded by RBB1 contained two conserve domain ATP-synt-DE-N and AtpC from 3 to 83 and from 1 to 133. All above showed that the full length cDNA RBB1 encoded ATP synthase CF1 epsilon chain protein The plant resistant mechanism is very sophisticated and one of the mainly responses is the metabolic change.

植物的抗病机理非常复杂,其主要反应之一就是代谢上的变化,例如,在利用cDNA微阵列对水稻抗稻瘟病近等基因系的分析中,检测到了很多与光合作用和糖代谢功能有关的差异表达片段;在小麦中也筛选到一个植物中ATP酶具有高度同源性的cDNA序列可能与小麦抗白粉病相关。

In this study, we screened antibacterial activities of 50% ethanolic extracts 87 traditional Chinese medicines to penicillin-resistant Staphylococcus simulans, and used disc agar diffusion method, broth dilution method determination of minimum inhibitory concentrations. The results showed that Equisetum hiemale L.

在这项研究,我们筛选了经50%乙醇萃取的八十七种传统中药材对产生青霉素抗药性Staphylococcus simulans进行抗菌活性检测,使用琼脂纸锭扩散法和连续稀释法来判断最小抑制浓度,结果发现木贼Equisetum hiemale L。

Single factor and orthogonal design were respectively utilized to optimize extracting process of ergosterol from fermentated rice of Monascus.

通过单因素试验和正交优化试验筛选由红曲提取麦角甾醇的最佳条件。

Yeast is one of the main ergosterol producers. Therefore, the ergosterol content should be increased as high as possible for application in ergosterol production.

野生型的酵母菌株在细胞生长和甾醇合成之间常存在一定的矛盾,因此通过自然筛选很难获得生长良好和麦角甾醇含量高的菌株。

The essentialist glorification of the profession notion and the evolutionist track of professionalization correspond with modernity, which is interlaced with social work; social structure and theories of profession provide the blueprint of its professionalized practices.

社会工作概论课本作为对於新成员初步社会化的桥梁,无论是理论的筛选、疏漏与应用,抑或文本结构的安排,「社会工作──专业」的相关论述呈现高度的同质与连贯性。

State Long Guoqiang, deputy director of Research Center, revealed to reporters the State Research Center of the pilot cities are being screened, and will be started in the middle of this year simulation run, using "real data, idling to run" approach, in order for the eventual introduction of real estate duty to provide the basis for decision-making.

国家隆国强,副主任研究中心,向记者透露,国家研究中心的试点城市正在筛选,将开始于今年年中模拟运行,采用"真实数据,空转运行"的方式,在为了最终引进房地产税提供决策依据。

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推荐网络例句

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。