筛选

Results showed that specific SIEA26~28 ku scFv with high level expression was achieved. The corresponding gene, ribosomal protein S4 was obtained by initially screening the cercaria cDNA library with the specific SIEA26~28 ku scFv. The obtaining of specific SIEA26~28 ku scFv lays the foundation for further screening and identification of the coding genes of SIEA26~28 ku, the natural molecular candidate vaccine against schistosomiasis.

结果显示，所获得的SIEA26~28ku特异性scFv，表达量高，采用该探针初步筛选出相关基因核糖体蛋白S4SIEA26~28ku特异性scFv的获得，为进一步筛选、分析鉴定抗日本血吸虫病天然分子候选疫苗SIEA26~28ku的编码基因奠定了基础。

Scientists will be screening chickpea and wheat collections in Pakistan for traits of economic importance for farmers; characterizing rare coconuts in Sri Lanka for traits of drought tolerance and tolerance to other pests and diseases; screening for salinity tolerance in sweet potatoes in Peru; and identifying drought-tolerant bananas in India.

科学家们将筛选鹰嘴豆，小麦收藏在巴基斯坦的性状的经济重要性的农民；特点罕见的椰子在斯里兰卡的性状的耐旱性和容忍其他害虫和疾病；筛选耐盐性的红薯，秘鲁，并确定乾旱容错香蕉在印度。

The invention provides a method for producing transgene sugarcane quickly with pmi genes. The method primarily comprises the steps of establishing a mannitose screening system; transformation of a gene gun; acquisition of resistant callus; differentiation and screening of the resistant callus; acquisition and rootage of resistant plantlets; molecule detection of resistant plantlets regenerated and detection of pmi gene expression by the chlorophenol red detection method.

一种利用pmi基因快速获得转基因甘蔗的方法，主要内容包括：甘露糖筛选体系的建立，基因枪转化和抗性愈伤组织的获得，抗性愈伤组织的分化筛选，抗性苗的获得和生根，抗性再生苗的分子检测和氯酚红法检测法检测pmi基因表达。

Therefore this dissertation studies the macrocosmic evaluate methods of bus priority planning based on the contents above, and constitutes a set of evaluation indexes using the managers, users and relatives as the main bodies of the plan evaluation. It filters a more relative indexes by the method of independent minimum variable fuzzy cluster analysis and analyzes the calculate methods of the chosen indexes.

确立以建设管理者、使用者和相关者作为方案评价的主体，构造了相应的评价指标，采用独立最小变量模糊聚类方法对相关性较强的指标进行了筛选，并对筛选后的各个指标的计算方法进行了分析。

We now also support the ability to filter by colour color.

在excel12中，我们能做的已超出了对单元格中数值的筛选，现在我们还能通过给单元格涂颜色进行筛选。

In Excel 12, we have gone beyond the ability to filter on values in cells. We now also support the ability to filter by colour color.

在excel12中，我们能做的已超出了对单元格中数值的筛选，现在我们还能通过给单元格涂颜色进行筛选。

That analysis reealed good congruency, with r = 0.998 for unselected HCTs and r = 0.999 for selected HCTs.

分析显示完全一致，未筛选的造血干细胞移植物的相关系数r = 0.998；筛选的造血干细胞移植物的相关系数r = 0.999。

PartⅡTransform the yeast, screen the excellent expression yeast strain, induce the yeast to express the fusion protein and depurate the target protein. The recombination plasmid was transformed into the yeast GS115 successfully by means of electrotransformation. The excellent yeast strain was got by G418 screening. We achived the yeast supernatant including target protein induced by methanol.

第二部分：酵母的转化、蛋白高表达菌株的筛选、重组蛋白的诱导表达及纯化实验利用电转化的方法，成功地将重组质粒pPIC9k-PTD-hA20导入毕赤酵母GS115，并通过G418浓度梯度筛选得到了蛋白高表达菌株，并对蛋白高表达菌株进行甲醇诱导表达，得到了含有目的蛋白的酵母上清液。

Objective To compare the performance of methods used in the microarray data analysis for selecting differentially expressed genes.

目的 比较基因芯片数据分析中常用的用于筛选差异表达基因的几种方法，探讨各方法的筛选效果。

F〓 of transgenic allotetraploid fish containing"all-fish"transgene——pCAgcGHc was obtained respectively by inbreeding and gynogenesis. Positive rate of pCAgcGHc detected by PCR was 90％ in F〓 of transgenic allotetraploid fish produced by inbreeding, while most F〓 of transgenic allotetraploid fish produced by gynogenesis showed diploidy measured by flow cytometer. The results above imply that developing the pure line of transgenic allotetraploid fish through inbreeding is feasible, but the operation should be modified if gynogenesis is introduced.

采用近交和雌核发育方法培育出转pCAgcGHc基因异源四倍体鲫鲤F〓代，通过PCR检测，外源基因pCAgcGHc在近交转pCAgcGHc基因异源四倍体鲫鲤F〓中的整合率为90％，而流式细胞技术检测雌核发育转pCAgcGHc基因异源四倍体鲫鲤F〓的倍性多为二倍体，说明通过近交筛选转基因异源四倍体鲫鲤纯系的方法是可行的，但是通过雌核发育来筛选转基因异源四倍体鲫鲤纯系还要就方法上进一步的改进。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。