筛选

Objective To immunoscreen one protein antigen gene from a cDNA library of Cysticercus cellulosae . Methods A cDNA library of C.

目的 从猪囊尾蚴cDNA文库中筛选出编码某种蛋白抗原的基因。

By using of transgenesis, cell mutagenesis, analysis of signal pathways and genes interaction, we developed novel methods of system biotechnology for drug discovery, transgenic bioreactor and isolation of differential expression genes involved in cytogenesis .

采用转基因细胞系、突变细胞系研究细胞发生的信号传导路径、多基因相互作用与生物系统的结构–图式形成，发展了系统生物学新技术进行基因克隆、药物筛选和生物反应器的方法。

Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

Abstract］ AIM: To construct recombinant vectors expressing siRNA that target CD59 gene and a stableinhibit cell line A2780 in order to analyze the role of CD59 in the protection to complementmediated cytolysis.

摘 要］目的：构建针对人CD59基因的siRNA表达载体并筛选稳定细胞系，观察CD59基因改变后对补体溶破的抵抗作用的变化，探讨CD59在肿瘤细胞免疫逃逸及相关信号转导通路中的作用。

Objective: To investigate immunostimulatory activity of CpG ODN against immunosuppression mice, aged mice, pregnant mice and their newly born mice; To screen a CpG ODN that its immunostimulatory activity against human cord blood lymphocytes is stronger than others; To observe immunostimulatory activity of CpG ODN against premature infants cord blood lymphocytes and to observe its cytolysis against Kse2 cells.

摘要目的：探讨CpG ODN对免疫抑制小鼠、老年鼠、孕鼠及其新生小鼠的免疫刺激作用；筛选对人脐血淋巴细胞免疫刺激作用较强的一种CpG ODN；观察其对早产儿脐血淋巴细胞的免疫刺激作用及刺激NK细胞对K_(562)细胞的杀伤活性，为开发适合人类新型疫苗佐剂奠定基础。

PSE7 and SOJB had the similar binding proteins in tobacco, suggesting that they may share some similar biological functions. The ubiquitin-conjugating protein and peptidase involved in protein degradation were found binding with SOJB but not PSE7. Both SOJB and PSE7 could bind with transmembrane transport protein, SOJB bound with copper transporter protein, and PSE7 bound with water channel protein. The results suggested that the target of SOJB and PSE7 may be located in cytoplast.

PSE7和SOJB在烟草中存在几个相似的结合蛋白，表明二者具有共享的生物学功能；SOJB能与肽酶和泛素结合蛋白（与蛋白质降解有密切关系）互作，而PSE7没有筛选到类似蛋白；SOJB和PSE7均能与跨膜运输蛋白结合，PSE7与水通道蛋白结合，SOJB与铜离子转运蛋白结合，表明二者在胞质内均存在作用靶标。

The purpose builds uremic patient to choose model of a collection of illustrative plates of serum protein dactylogram.

概要： 目的建立尿毒症患者筛选血清蛋白质指纹图谱模型。

The model of a collection of illustrative plates of serum protein dactylogram that conclusion builds can distinguish uremic patient and health person, the respect such as the filtration of the SELDI-TOF-MS diagnosis in uremia and element of mark of particularity protein biology has fair value.

结论建立的血清蛋白质指纹图谱模型能够区分尿毒症患者和健康者，SELDI-TOF-MS在尿毒症的诊断及特异性蛋白质生物标志分子的筛选等方面具有一定价值。

Come back daily to see the editors' selection of photographs in the Daily Dozen.

每天到"每日十二幅"来看看编辑筛选出的照片。

Damping-off, as well as screen antagonistic actinomycetes of Pythium aphanidermatum.

猝倒病的病原菌，筛选可用于甜瓜猝倒病生物防治的拮抗性放线菌。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。